Hair cortisol, testosterone, dehydroepiandrosterone sulfate and their ratios in stallions as a retrospective measure of hypothalamic-pituitary-adrenal and hypothalamic-pituitary- gonadal axes activity

The monitoring of stress physiology includes studying a wide range of endocrinological mechanisms, which can be assessed using multiple tissue samples. This study aimed to evaluate the seasonal variations of hair C, T and DHEA-S in horses for a whole year, as well as to assess the variations between...

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Detalles Bibliográficos
Autores: Olvera-Maneu, Sergi|||0000-0003-4281-6510, Carbajal, Annaïs|||0000-0002-4339-6661, Gardela Santacruz, Jaume|||0000-0001-7524-2088, López Béjar, Manel|||0000-0001-9490-6126
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:250387
Acceso en línea:https://ddd.uab.cat/record/250387
https://dx.doi.org/urn:doi:10.3390/ani11082202
Access Level:acceso abierto
Palabra clave:Hair
Testosterone
Cortisol
Dehydroepiandrosterone sulphate
Ratio
Season
Horse
Stallion
Non-invasive
Hypothalamic-pituitary-adrenal axis
Hypothalamic-pituitary-gonadal axis
Descripción
Sumario:The monitoring of stress physiology includes studying a wide range of endocrinological mechanisms, which can be assessed using multiple tissue samples. This study aimed to evaluate the seasonal variations of hair C, T and DHEA-S in horses for a whole year, as well as to assess the variations between seasons of C/DHEA-S and T/C ratios as a retrospective measure of the hypothalamic-pituitary-adrenal and hypothalamic-pituitary-gonadal axis activity. Ten pure-breed Menorca stallions were included in the study. The hair samples were collected approximately every two months following the shave-reshave method caudally to the sternum. After a methanol-based extraction, samples were analyzed by enzyme immunoassay for cortisol, testosterone, and dehy-droepiandrosterone sulphate. Following our findings, we detected that cortisol, testosterone and dehydroepiandrosterone sulphate were significantly affected by seasonality, with the highest values of cortisol during summer and the lowest values of testosterone during spring. Dehydroepiandros-terone sulphate concentrations were increased in autumn compared to the other studied periods. Additionally, the studied hormone ratios showed variations between seasons. To conclude, season should, therefore, be considered when assessing sexual and stress hormones in stallion hair, since this variable can be a potential influencing factor and led to misinterpretations.