Seminal extracellular vesicles subsets modulate gene expression in cumulus cells of porcine in vitro matured oocytes

Seminal plasma (SP), a fluid composed mainly by secretions from accessory sex glands, contains a heterogenous population of extracellular vesicles (EVs), involved in several reproductive physiological processes. Seminal plasma has been found to modulate ovary function, in terms of hormone secretion...

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Autores: Mateo Otero, Yentel, Yeste Oliveras, Marc, Roca, Jordi, Llavanera, Marc, Bucci, Diego, Galeati, Giovanna, Spinaci, Marcella, Barranco, Isabel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/22098
Acceso en línea:http://hdl.handle.net/10256/22098
Access Level:acceso abierto
Palabra clave:Expressió gènica
Fecundació in vitro
Gene expression
Fertilization in vitro
Porcs -- Reproducció
Swine -- Reproduction
Porcs -- Espermatozoides -- Investigació
Swine -- Spermatozoa -- Research
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oai_identifier_str oai:recercat.cat:10256/22098
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network_name_str España
repository_id_str
spelling Seminal extracellular vesicles subsets modulate gene expression in cumulus cells of porcine in vitro matured oocytesMateo Otero, YentelYeste Oliveras, MarcRoca, JordiLlavanera, MarcBucci, DiegoGaleati, GiovannaSpinaci, MarcellaBarranco, IsabelExpressió gènicaFecundació in vitroGene expressionFertilization in vitroPorcs -- ReproduccióSwine -- ReproductionPorcs -- Espermatozoides -- InvestigacióSwine -- Spermatozoa -- ResearchSeminal plasma (SP), a fluid composed mainly by secretions from accessory sex glands, contains a heterogenous population of extracellular vesicles (EVs), involved in several reproductive physiological processes. Seminal plasma has been found to modulate ovary function, in terms of hormone secretion and immune regulation. This study evaluated the potential effect of SP-EV-subsets on the modulation of cumulus-oocyte-complex (COCs) physiology during in vitro maturation (IVM). Two SP-EV-subsets, small-EVs (S-EVs) and large-EVs (L-EVs), were isolated from pig SP by size-exclusion-chromatography. Next, COCs were IVM in the absence (control) or presence of each SP-EV-subset to evaluate their uptake by COCs (PKH67-EVs labelling) and their effect on oocyte and cumulus cells (CCs) (gene expression, and progesterone and estradiol-17β levels). S-EVs and L-EVs were able to bind CCs but not oocytes. Supplementation with L-EVs induced changes (P ≤ 0.05) in the transcript levels of oocyte maturation- (HAS2) and steroidogenesis-related genes (CYP11A1 and HSD3B1) in CCs. No effect on nuclear oocyte maturation and progesterone and estradiol-17β levels was observed when COCs were IVM with any of the two SP-EV-subsets. In conclusion, while SP-EV-subsets can be integrated by CCs during IVM, they do not affect oocyte maturation and only L-EVs are able to modulate CCs function, mainly modifying the expression of steroidogenesis-related genesThis research was supported by the European Commission (Horizon 2020 Research and Innovation programme; H2020-MSCA-IF-2019–891382), European Union – NextGenerationEU - ALMArie CURIE 2021 SupER - University of Bologna funded by Italian Ministry of University and Research (D.M. 737/2021)(CUP: J45F21001470005), the Ministry of Science and Innovation, Spain (Grant PID2020-113493RB-I00 founded by MCIN/AEI/10.13039/501100011033), the Catalan Agency for Management of University and Research Grants, Regional Government of Catalonia, Spain (2017-SGR-1229 and 2020-FI-B-00412), and the Catalan Institution for Research and Advanced Studies (ICREA)Nature Publishing Group2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer-reviewedapplication/pdfhttp://hdl.handle.net/10256/22098http://hdl.handle.net/10256/22098Scientific Reports, 2022, vol. 12, art.núm. 19096Articles publicats (D-B)Mateo Otero, Yentel Yeste Oliveras, Marc Roca, Jordi Llavanera, Marc Bucci, Diego Galeati, Giovanna Spinaci, Marcella Barranco, Isabel 2022 Seminal extracellular vesicles subsets modulate gene expression in cumulus cells of porcine in vitro matured oocytes Scientific Reports 12 art.núm. 19096reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-022-22004-7info:eu-repo/semantics/altIdentifier/eissn/2045-2322Attribution 4.0 International (CC BY 4.0)https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10256/220982026-05-29T05:05:01Z
dc.title.none.fl_str_mv Seminal extracellular vesicles subsets modulate gene expression in cumulus cells of porcine in vitro matured oocytes
title Seminal extracellular vesicles subsets modulate gene expression in cumulus cells of porcine in vitro matured oocytes
spellingShingle Seminal extracellular vesicles subsets modulate gene expression in cumulus cells of porcine in vitro matured oocytes
Mateo Otero, Yentel
Expressió gènica
Fecundació in vitro
Gene expression
Fertilization in vitro
Porcs -- Reproducció
Swine -- Reproduction
Porcs -- Espermatozoides -- Investigació
Swine -- Spermatozoa -- Research
title_short Seminal extracellular vesicles subsets modulate gene expression in cumulus cells of porcine in vitro matured oocytes
title_full Seminal extracellular vesicles subsets modulate gene expression in cumulus cells of porcine in vitro matured oocytes
title_fullStr Seminal extracellular vesicles subsets modulate gene expression in cumulus cells of porcine in vitro matured oocytes
title_full_unstemmed Seminal extracellular vesicles subsets modulate gene expression in cumulus cells of porcine in vitro matured oocytes
title_sort Seminal extracellular vesicles subsets modulate gene expression in cumulus cells of porcine in vitro matured oocytes
dc.creator.none.fl_str_mv Mateo Otero, Yentel
Yeste Oliveras, Marc
Roca, Jordi
Llavanera, Marc
Bucci, Diego
Galeati, Giovanna
Spinaci, Marcella
Barranco, Isabel
author Mateo Otero, Yentel
author_facet Mateo Otero, Yentel
Yeste Oliveras, Marc
Roca, Jordi
Llavanera, Marc
Bucci, Diego
Galeati, Giovanna
Spinaci, Marcella
Barranco, Isabel
author_role author
author2 Yeste Oliveras, Marc
Roca, Jordi
Llavanera, Marc
Bucci, Diego
Galeati, Giovanna
Spinaci, Marcella
Barranco, Isabel
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Expressió gènica
Fecundació in vitro
Gene expression
Fertilization in vitro
Porcs -- Reproducció
Swine -- Reproduction
Porcs -- Espermatozoides -- Investigació
Swine -- Spermatozoa -- Research
topic Expressió gènica
Fecundació in vitro
Gene expression
Fertilization in vitro
Porcs -- Reproducció
Swine -- Reproduction
Porcs -- Espermatozoides -- Investigació
Swine -- Spermatozoa -- Research
description Seminal plasma (SP), a fluid composed mainly by secretions from accessory sex glands, contains a heterogenous population of extracellular vesicles (EVs), involved in several reproductive physiological processes. Seminal plasma has been found to modulate ovary function, in terms of hormone secretion and immune regulation. This study evaluated the potential effect of SP-EV-subsets on the modulation of cumulus-oocyte-complex (COCs) physiology during in vitro maturation (IVM). Two SP-EV-subsets, small-EVs (S-EVs) and large-EVs (L-EVs), were isolated from pig SP by size-exclusion-chromatography. Next, COCs were IVM in the absence (control) or presence of each SP-EV-subset to evaluate their uptake by COCs (PKH67-EVs labelling) and their effect on oocyte and cumulus cells (CCs) (gene expression, and progesterone and estradiol-17β levels). S-EVs and L-EVs were able to bind CCs but not oocytes. Supplementation with L-EVs induced changes (P ≤ 0.05) in the transcript levels of oocyte maturation- (HAS2) and steroidogenesis-related genes (CYP11A1 and HSD3B1) in CCs. No effect on nuclear oocyte maturation and progesterone and estradiol-17β levels was observed when COCs were IVM with any of the two SP-EV-subsets. In conclusion, while SP-EV-subsets can be integrated by CCs during IVM, they do not affect oocyte maturation and only L-EVs are able to modulate CCs function, mainly modifying the expression of steroidogenesis-related genes
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
peer-reviewed
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10256/22098
http://hdl.handle.net/10256/22098
url http://hdl.handle.net/10256/22098
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-022-22004-7
info:eu-repo/semantics/altIdentifier/eissn/2045-2322
dc.rights.none.fl_str_mv Attribution 4.0 International (CC BY 4.0)
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International (CC BY 4.0)
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv Scientific Reports, 2022, vol. 12, art.núm. 19096
Articles publicats (D-B)
Mateo Otero, Yentel Yeste Oliveras, Marc Roca, Jordi Llavanera, Marc Bucci, Diego Galeati, Giovanna Spinaci, Marcella Barranco, Isabel 2022 Seminal extracellular vesicles subsets modulate gene expression in cumulus cells of porcine in vitro matured oocytes Scientific Reports 12 art.núm. 19096
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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