A label-free diffraction-based sensing displacement immunosensor toquantify low molecular weight organic compounds

[EN] Herein we present a diffractometric immunosensor to quantify low molecular weight organic compounds in a label-free, simple, and sensitive fashion. The approach is based on patterning analyte analogues (haptens) on solid surfaces according to a diffractive structure, and then loading specific a...

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Detalles Bibliográficos
Autores: Avella-Oliver, Miquel|||0000-0002-7293-6989, Ferrando, Vicente|||0000-0002-7434-7109, Monsoriu Serra, Juan Antonio|||0000-0003-3350-7951, Puchades, Rosa|||0000-0002-9329-1593, Maquieira, Angel|||0000-0003-4641-4957
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/117898
Acceso en línea:https://riunet.upv.es/handle/10251/117898
Access Level:acceso abierto
Palabra clave:Biograting
Diffraction-based sensing
Atrazine
Label-free
Microcontact printing
Displacement immunoassay
QUIMICA ANALITICA
MATEMATICA APLICADA
FISICA APLICADA
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repository_id_str
spelling A label-free diffraction-based sensing displacement immunosensor toquantify low molecular weight organic compoundsAvella-Oliver, Miquel|||0000-0002-7293-6989Ferrando, Vicente|||0000-0002-7434-7109Monsoriu Serra, Juan Antonio|||0000-0003-3350-7951Puchades, Rosa|||0000-0002-9329-1593Maquieira, Angel|||0000-0003-4641-4957BiogratingDiffraction-based sensingAtrazineLabel-freeMicrocontact printingDisplacement immunoassayQUIMICA ANALITICAMATEMATICA APLICADAFISICA APLICADA[EN] Herein we present a diffractometric immunosensor to quantify low molecular weight organic compounds in a label-free, simple, and sensitive fashion. The approach is based on patterning analyte analogues (haptens) on solid surfaces according to a diffractive structure, and then loading specific antibodies on them to be subsequently displaced by free analytes in solution. This displacement generates a measurable change in the diffractive response that enables to quantify the analyte concentration. In this study we address the fabrication, optimization, and assessment of these diffractive structures of biological probes and their application to the analysis of atrazine, an organic compound extensively used as pesticide. This immunosensor displays well-correlated dose-response curves that reach a detection limit of 1.1¿ng¿mL¿1 of atrazine in label-free conditions. From a general viewpoint, this study also aims to provide insights into exploiting this approach towards prospective in-field analysis and screening strategies to sense multiple low molecular weight compounds in label-free conditions.This work was supported by the Spanish Ministry of Economy and Competitiveness (CTQ2013-45875-R and FIS2011-23175), FEDER, and the Generalitat Valenciana (PROMETEO II/2014/040 and PROMETEO II/2014/072). Special thanks go to Richard A. McAloney and M. Cynthia Goh for hosting M.A.-O. as visiting researcher, sharing their expertise, and offering their valuable support. M.A.-O. also acknowledges the FPI program of the Spanish Ministry of Economy and Competitiveness for a PhD and an EEBB mobility grant.ElsevierDepartamento de Física AplicadaEscuela Técnica Superior de Ingeniería Aeroespacial y Diseño IndustrialDepartamento de QuímicaInstituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo TecnológicoCentro de Tecnologías Físicas: Acústica, Materiales y AstrofísicaEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalEscuela de DoctoradoGeneralitat ValencianaMinisterio de Economía y CompetitividadMinisterio de Ciencia e InnovaciónRepositorio Institucional de la Universitat Politècnica de València Riunet20182018-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://riunet.upv.es/handle/10251/117898reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 CTQ2013-45875-R BIOSENSADO EN SOPORTES INTERACTIVOS CON PROPIEDADES INTERFEROMETRICAS PARA APLICACIONES CLINICAS. DEMOSTRACION EN FARMACOGENETICA Y ALERGIAS MEDICAMENTOSASMinisterio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 FIS2011-23175 DISEÑO Y REALIZACION DE ESTRUCTURAS DIFRACTIVAS APERIODICAS: NUEVAS LENTES OFTALMICAS Y OTRAS APLICACIONESGeneralitat Valenciana https://doi.org/10.13039/501100003359 PROMETEOII%2F2014%2F040 Estudio de estrategias fisico-químicas para el desarrollo de biosensores interferométricos en soportes interactivos de aplicación en clínicaGeneralitat Valenciana https://doi.org/10.13039/501100003359 PROMETEOII%2F2014%2F072 Grupo de fibras ópticas y procesado de señalopen accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/1178982026-06-13T07:49:27Z
dc.title.none.fl_str_mv A label-free diffraction-based sensing displacement immunosensor toquantify low molecular weight organic compounds
title A label-free diffraction-based sensing displacement immunosensor toquantify low molecular weight organic compounds
spellingShingle A label-free diffraction-based sensing displacement immunosensor toquantify low molecular weight organic compounds
Avella-Oliver, Miquel|||0000-0002-7293-6989
Biograting
Diffraction-based sensing
Atrazine
Label-free
Microcontact printing
Displacement immunoassay
QUIMICA ANALITICA
MATEMATICA APLICADA
FISICA APLICADA
title_short A label-free diffraction-based sensing displacement immunosensor toquantify low molecular weight organic compounds
title_full A label-free diffraction-based sensing displacement immunosensor toquantify low molecular weight organic compounds
title_fullStr A label-free diffraction-based sensing displacement immunosensor toquantify low molecular weight organic compounds
title_full_unstemmed A label-free diffraction-based sensing displacement immunosensor toquantify low molecular weight organic compounds
title_sort A label-free diffraction-based sensing displacement immunosensor toquantify low molecular weight organic compounds
dc.creator.none.fl_str_mv Avella-Oliver, Miquel|||0000-0002-7293-6989
Ferrando, Vicente|||0000-0002-7434-7109
Monsoriu Serra, Juan Antonio|||0000-0003-3350-7951
Puchades, Rosa|||0000-0002-9329-1593
Maquieira, Angel|||0000-0003-4641-4957
author Avella-Oliver, Miquel|||0000-0002-7293-6989
author_facet Avella-Oliver, Miquel|||0000-0002-7293-6989
Ferrando, Vicente|||0000-0002-7434-7109
Monsoriu Serra, Juan Antonio|||0000-0003-3350-7951
Puchades, Rosa|||0000-0002-9329-1593
Maquieira, Angel|||0000-0003-4641-4957
author_role author
author2 Ferrando, Vicente|||0000-0002-7434-7109
Monsoriu Serra, Juan Antonio|||0000-0003-3350-7951
Puchades, Rosa|||0000-0002-9329-1593
Maquieira, Angel|||0000-0003-4641-4957
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Física Aplicada
Escuela Técnica Superior de Ingeniería Aeroespacial y Diseño Industrial
Departamento de Química
Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico
Centro de Tecnologías Físicas: Acústica, Materiales y Astrofísica
Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural
Escuela de Doctorado
Generalitat Valenciana
Ministerio de Economía y Competitividad
Ministerio de Ciencia e Innovación
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Biograting
Diffraction-based sensing
Atrazine
Label-free
Microcontact printing
Displacement immunoassay
QUIMICA ANALITICA
MATEMATICA APLICADA
FISICA APLICADA
topic Biograting
Diffraction-based sensing
Atrazine
Label-free
Microcontact printing
Displacement immunoassay
QUIMICA ANALITICA
MATEMATICA APLICADA
FISICA APLICADA
description [EN] Herein we present a diffractometric immunosensor to quantify low molecular weight organic compounds in a label-free, simple, and sensitive fashion. The approach is based on patterning analyte analogues (haptens) on solid surfaces according to a diffractive structure, and then loading specific antibodies on them to be subsequently displaced by free analytes in solution. This displacement generates a measurable change in the diffractive response that enables to quantify the analyte concentration. In this study we address the fabrication, optimization, and assessment of these diffractive structures of biological probes and their application to the analysis of atrazine, an organic compound extensively used as pesticide. This immunosensor displays well-correlated dose-response curves that reach a detection limit of 1.1¿ng¿mL¿1 of atrazine in label-free conditions. From a general viewpoint, this study also aims to provide insights into exploiting this approach towards prospective in-field analysis and screening strategies to sense multiple low molecular weight compounds in label-free conditions.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/117898
url https://riunet.upv.es/handle/10251/117898
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 CTQ2013-45875-R BIOSENSADO EN SOPORTES INTERACTIVOS CON PROPIEDADES INTERFEROMETRICAS PARA APLICACIONES CLINICAS. DEMOSTRACION EN FARMACOGENETICA Y ALERGIAS MEDICAMENTOSAS
Ministerio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 FIS2011-23175 DISEÑO Y REALIZACION DE ESTRUCTURAS DIFRACTIVAS APERIODICAS: NUEVAS LENTES OFTALMICAS Y OTRAS APLICACIONES
Generalitat Valenciana https://doi.org/10.13039/501100003359 PROMETEOII%2F2014%2F040 Estudio de estrategias fisico-químicas para el desarrollo de biosensores interferométricos en soportes interactivos de aplicación en clínica
Generalitat Valenciana https://doi.org/10.13039/501100003359 PROMETEOII%2F2014%2F072 Grupo de fibras ópticas y procesado de señal
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento - No comercial - Sin obra derivada (by-nc-nd)
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento - No comercial - Sin obra derivada (by-nc-nd)
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301603

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