White adipose tissue-infiltrated CD11b+ myeloid cells are a source of S100A4, a new potential marker of hepatic damage

Context The endocrine and immunological properties of subcutaneous vs. visceral adipose tissue (sWAT and vWAT, respectively) have turned a milestone in the study of metabolic diseases. The cytokine S100A4 has been reported to be elevated in obesity and to have a role in adipose tissue dysfunction. H...

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Autores: Reyes, Majorie, González, Lorena, Ibeas, Kevin, Cereijo Téllez, Rubén, Taxerås, Siri D., Pellitero, Silvia, Martínez, Eva, Tarascó, Jordi, Moreno, Pau, Malagón, Paloma, Higueras, Carmen, Soria, Andrea, Puig Domingo, Manuel, Villarroya i Gombau, Francesc, Serra i Cucurull, Dolors, Herrero Rodríguez, Laura, Sánchez-Infantes, David
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2021
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/176327
Acceso en línea:https://hdl.handle.net/2445/176327
Access Level:acceso abierto
Palabra clave:Obesitat
Proteïnes
Teixit adipós
Trastorns del metabolisme
Obesity
Proteins
Adipose tissues
Disorders of metabolism
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spelling White adipose tissue-infiltrated CD11b+ myeloid cells are a source of S100A4, a new potential marker of hepatic damageReyes, MajorieGonzález, LorenaIbeas, KevinCereijo Téllez, RubénTaxerås, Siri D.Pellitero, SilviaMartínez, EvaTarascó, JordiMoreno, PauMalagón, PalomaHigueras, CarmenSoria, AndreaPuig Domingo, ManuelVillarroya i Gombau, FrancescSerra i Cucurull, DolorsHerrero Rodríguez, LauraSánchez-Infantes, DavidObesitatProteïnesTeixit adipósTrastorns del metabolismeObesityProteinsAdipose tissuesDisorders of metabolismContext The endocrine and immunological properties of subcutaneous vs. visceral adipose tissue (sWAT and vWAT, respectively) have turned a milestone in the study of metabolic diseases. The cytokine S100A4 has been reported to be elevated in obesity and to have a role in adipose tissue dysfunction. However, the cellular source in adipose tissue and its potential role in hepatic damage in obesity has not been elucidated. Objective We aim to study the regulation of S100A4 in immune cells present in sWAT and vWAT, as well as its potential role as a circulating marker of hepatic inflammation and steatosis. Design A cohort of 60 patients with obesity and distinct metabolic status was analyzed. CD11b+ myeloid cells and T cells were isolated from sWAT and vWAT by magnetic-activating cell sorting, and RNA was obtained. S100A4 gene expression was measured, and correlation analysis with clinical data was performed. Liver biopsies were obtained from 20 patients, and S100A4 circulating levels were measured to check the link with hepatic inflammation and steatosis. Results S100A4 gene expression was strongly upregulated in sWAT- vs. vWAT-infiltrated CD11b+ cells, but this modulation was not observed in T resident cells. S100A4 mRNA levels from sWAT (and not from vWAT) CD11b+ cells positively correlated with glycemia, triglycerides and TNF-α gene expression. Finally, circulating S100A4 directly correlated with liver steatosis and hepatic inflammatory markers. Conclusion Our data suggest that sWAT-infiltrated CD11b+ cells could be a major source of S100A4 in obesity. Moreover, our correlations identify circulating S100A4 as a potential novel biomarker of hepatic damage and steatosis.Bioscientifica2021202220212021info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion9 p.application/pdfhttps://hdl.handle.net/2445/176327Articles publicats en revistes (Bioquímica i Fisiologia)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: https://doi.org/10.1530/EJE-20-1130European Journal of Endocrinology, 2021, vol. 184, num. 4, p. 533-541https://doi.org/10.1530/EJE-20-1130(c) European Society of Endocrinology, 2021info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1763272026-05-29T05:05:01Z
dc.title.none.fl_str_mv White adipose tissue-infiltrated CD11b+ myeloid cells are a source of S100A4, a new potential marker of hepatic damage
title White adipose tissue-infiltrated CD11b+ myeloid cells are a source of S100A4, a new potential marker of hepatic damage
spellingShingle White adipose tissue-infiltrated CD11b+ myeloid cells are a source of S100A4, a new potential marker of hepatic damage
Reyes, Majorie
Obesitat
Proteïnes
Teixit adipós
Trastorns del metabolisme
Obesity
Proteins
Adipose tissues
Disorders of metabolism
title_short White adipose tissue-infiltrated CD11b+ myeloid cells are a source of S100A4, a new potential marker of hepatic damage
title_full White adipose tissue-infiltrated CD11b+ myeloid cells are a source of S100A4, a new potential marker of hepatic damage
title_fullStr White adipose tissue-infiltrated CD11b+ myeloid cells are a source of S100A4, a new potential marker of hepatic damage
title_full_unstemmed White adipose tissue-infiltrated CD11b+ myeloid cells are a source of S100A4, a new potential marker of hepatic damage
title_sort White adipose tissue-infiltrated CD11b+ myeloid cells are a source of S100A4, a new potential marker of hepatic damage
dc.creator.none.fl_str_mv Reyes, Majorie
González, Lorena
Ibeas, Kevin
Cereijo Téllez, Rubén
Taxerås, Siri D.
Pellitero, Silvia
Martínez, Eva
Tarascó, Jordi
Moreno, Pau
Malagón, Paloma
Higueras, Carmen
Soria, Andrea
Puig Domingo, Manuel
Villarroya i Gombau, Francesc
Serra i Cucurull, Dolors
Herrero Rodríguez, Laura
Sánchez-Infantes, David
author Reyes, Majorie
author_facet Reyes, Majorie
González, Lorena
Ibeas, Kevin
Cereijo Téllez, Rubén
Taxerås, Siri D.
Pellitero, Silvia
Martínez, Eva
Tarascó, Jordi
Moreno, Pau
Malagón, Paloma
Higueras, Carmen
Soria, Andrea
Puig Domingo, Manuel
Villarroya i Gombau, Francesc
Serra i Cucurull, Dolors
Herrero Rodríguez, Laura
Sánchez-Infantes, David
author_role author
author2 González, Lorena
Ibeas, Kevin
Cereijo Téllez, Rubén
Taxerås, Siri D.
Pellitero, Silvia
Martínez, Eva
Tarascó, Jordi
Moreno, Pau
Malagón, Paloma
Higueras, Carmen
Soria, Andrea
Puig Domingo, Manuel
Villarroya i Gombau, Francesc
Serra i Cucurull, Dolors
Herrero Rodríguez, Laura
Sánchez-Infantes, David
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Obesitat
Proteïnes
Teixit adipós
Trastorns del metabolisme
Obesity
Proteins
Adipose tissues
Disorders of metabolism
topic Obesitat
Proteïnes
Teixit adipós
Trastorns del metabolisme
Obesity
Proteins
Adipose tissues
Disorders of metabolism
description Context The endocrine and immunological properties of subcutaneous vs. visceral adipose tissue (sWAT and vWAT, respectively) have turned a milestone in the study of metabolic diseases. The cytokine S100A4 has been reported to be elevated in obesity and to have a role in adipose tissue dysfunction. However, the cellular source in adipose tissue and its potential role in hepatic damage in obesity has not been elucidated. Objective We aim to study the regulation of S100A4 in immune cells present in sWAT and vWAT, as well as its potential role as a circulating marker of hepatic inflammation and steatosis. Design A cohort of 60 patients with obesity and distinct metabolic status was analyzed. CD11b+ myeloid cells and T cells were isolated from sWAT and vWAT by magnetic-activating cell sorting, and RNA was obtained. S100A4 gene expression was measured, and correlation analysis with clinical data was performed. Liver biopsies were obtained from 20 patients, and S100A4 circulating levels were measured to check the link with hepatic inflammation and steatosis. Results S100A4 gene expression was strongly upregulated in sWAT- vs. vWAT-infiltrated CD11b+ cells, but this modulation was not observed in T resident cells. S100A4 mRNA levels from sWAT (and not from vWAT) CD11b+ cells positively correlated with glycemia, triglycerides and TNF-α gene expression. Finally, circulating S100A4 directly correlated with liver steatosis and hepatic inflammatory markers. Conclusion Our data suggest that sWAT-infiltrated CD11b+ cells could be a major source of S100A4 in obesity. Moreover, our correlations identify circulating S100A4 as a potential novel biomarker of hepatic damage and steatosis.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/176327
url https://hdl.handle.net/2445/176327
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1530/EJE-20-1130
European Journal of Endocrinology, 2021, vol. 184, num. 4, p. 533-541
https://doi.org/10.1530/EJE-20-1130
dc.rights.none.fl_str_mv (c) European Society of Endocrinology, 2021
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) European Society of Endocrinology, 2021
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 9 p.
application/pdf
dc.publisher.none.fl_str_mv Bioscientifica
publisher.none.fl_str_mv Bioscientifica
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Fisiologia)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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