Enzymatic synthesis of vanillin catalysed by an eugenol oxidase

Vanillin is one of the most important flavours produced in the world. Due to its increasing value and its demand, mainly in the food industry, several ways to obtain it at lower prices are under study. One of the routes is based on the oxidation of vanillyl alcohol by Eugenol oxidase (EUGO), which h...

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Detalles Bibliográficos
Autores: García Bofill, Miquel|||0000-0001-9458-346X, Sutton, Peter W., Guillén, Marina|||0000-0002-9740-9966, Álvaro, Gregorio|||0000-0002-2924-8902
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:217011
Acceso en línea:https://ddd.uab.cat/record/217011
https://dx.doi.org/urn:doi:10.1016/j.apcata.2019.117117
Access Level:acceso abierto
Palabra clave:Vanillin synthesis
Biocatalyst
Immobilisation
Eugenol oxidase
Natural flavour
Enzymatic biotransformation
Descripción
Sumario:Vanillin is one of the most important flavours produced in the world. Due to its increasing value and its demand, mainly in the food industry, several ways to obtain it at lower prices are under study. One of the routes is based on the oxidation of vanillyl alcohol by Eugenol oxidase (EUGO), which has high potential to be used at industrial scale owing to the high space time yields that can be obtained at lab scale (2.9 g L-1 h-1 of vanillin). Additionally, EUGO can be immobilised efficiently onto different supports (MANA-agarose, Epoxy-agarose and Purolite 8204F) which can be reused several times to perform the oxidation preserving good stability and improving more than 3-fold the biocatalyst yield.