Role of drug transporters in the sensitivity of acute myeloid leukemia to sorafenib

[EN]Chemoresistance often limits the success of the pharmacological treatment in acute myeloid leukemia (AML) patients. Although positive results have been obtained with tyrosine kinase inhibitors (TKIs), such as sorafenib, especially in patients with Fms-like tyrosine kinase 3 (FLT3)-positive AML,...

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Autores: Rodríguez Macías, Rocío Isabel, Sánchez Martín, Anabel, Rodríguez-Macías, Gabriela, Sánchez Abarca, Luis Ignacio, Lozano, Elisa, Herraez, Elisa, Odero, Maria D., Díez-Martín, José L., García Marín, José Juan, Briz Sánchez, Oscar
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Institución:Universidad de Salamanca (USAL)
Repositorio:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/146674
Acceso en línea:http://hdl.handle.net/10366/146674
Access Level:acceso abierto
Palabra clave:AML
Cancer
Chemoresistance
Chemotherapy
Tyrosine kinase inhibitor
Chemotherapy, Cancer, Regional Perfusion
Chemoreceptor Cells
3201.01 Oncología
3205.04 Hematología
quimioterapia del cáncer por perfusión regional
células quimiorreceptoras
síntomas de cáncer
id ES_a1cd16290eda94e9f07e4f9bf4e4809d
oai_identifier_str oai:gredos.usal.es:10366/146674
network_acronym_str ES
network_name_str España
repository_id_str
spelling Role of drug transporters in the sensitivity of acute myeloid leukemia to sorafenibRodríguez Macías, Rocío IsabelSánchez Martín, AnabelRodríguez-Macías, GabrielaSánchez Abarca, Luis IgnacioLozano, ElisaHerraez, ElisaOdero, Maria D.Díez-Martín, José L.García Marín, José JuanBriz Sánchez, OscarAMLCancerChemoresistanceChemotherapyTyrosine kinase inhibitorChemotherapy, Cancer, Regional PerfusionChemoreceptor Cells3201.01 Oncología3205.04 Hematologíaquimioterapia del cáncer por perfusión regionalcélulas quimiorreceptorassíntomas de cáncer[EN]Chemoresistance often limits the success of the pharmacological treatment in acute myeloid leukemia (AML) patients. Although positive results have been obtained with tyrosine kinase inhibitors (TKIs), such as sorafenib, especially in patients with Fms-like tyrosine kinase 3 (FLT3)-positive AML, the success of chemotherapy is very heterogeneous.The sensitivity to sorafenib-induced cell death (MTT test and anexin V/7-AAD method) was evaluated in five different cell lines: MOLM-13, OCI-AML2, HL-60, HEL and K-562. The transportome was characterized by measuring mRNA using RT-qPCR. Drug uptake/efflux was determined by flow cytometry using specific substrates and inhibitors. The cytostatic response to sorafenib was: MOLM-13»OCI-AML2>HL- 60>HEL≈K-562. Regarding efflux pumps, MDR1 was highly expressed in HEL>K- 562≈MOLM-13, but not in OCI-AML2 and HL-60. BCRP and MPR3 expression was low in all cell lines, whereas MRP4 and MRP5 expression was from moderate to high. Flow cytometry studies demonstrated that MRP4, but not MRP5, was functional.Impact Journals LLC202120212018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10366/146674reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)InglésAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:gredos.usal.es:10366/1466742026-06-07T06:28:51Z
dc.title.none.fl_str_mv Role of drug transporters in the sensitivity of acute myeloid leukemia to sorafenib
title Role of drug transporters in the sensitivity of acute myeloid leukemia to sorafenib
spellingShingle Role of drug transporters in the sensitivity of acute myeloid leukemia to sorafenib
Rodríguez Macías, Rocío Isabel
AML
Cancer
Chemoresistance
Chemotherapy
Tyrosine kinase inhibitor
Chemotherapy, Cancer, Regional Perfusion
Chemoreceptor Cells
3201.01 Oncología
3205.04 Hematología
quimioterapia del cáncer por perfusión regional
células quimiorreceptoras
síntomas de cáncer
title_short Role of drug transporters in the sensitivity of acute myeloid leukemia to sorafenib
title_full Role of drug transporters in the sensitivity of acute myeloid leukemia to sorafenib
title_fullStr Role of drug transporters in the sensitivity of acute myeloid leukemia to sorafenib
title_full_unstemmed Role of drug transporters in the sensitivity of acute myeloid leukemia to sorafenib
title_sort Role of drug transporters in the sensitivity of acute myeloid leukemia to sorafenib
dc.creator.none.fl_str_mv Rodríguez Macías, Rocío Isabel
Sánchez Martín, Anabel
Rodríguez-Macías, Gabriela
Sánchez Abarca, Luis Ignacio
Lozano, Elisa
Herraez, Elisa
Odero, Maria D.
Díez-Martín, José L.
García Marín, José Juan
Briz Sánchez, Oscar
author Rodríguez Macías, Rocío Isabel
author_facet Rodríguez Macías, Rocío Isabel
Sánchez Martín, Anabel
Rodríguez-Macías, Gabriela
Sánchez Abarca, Luis Ignacio
Lozano, Elisa
Herraez, Elisa
Odero, Maria D.
Díez-Martín, José L.
García Marín, José Juan
Briz Sánchez, Oscar
author_role author
author2 Sánchez Martín, Anabel
Rodríguez-Macías, Gabriela
Sánchez Abarca, Luis Ignacio
Lozano, Elisa
Herraez, Elisa
Odero, Maria D.
Díez-Martín, José L.
García Marín, José Juan
Briz Sánchez, Oscar
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv AML
Cancer
Chemoresistance
Chemotherapy
Tyrosine kinase inhibitor
Chemotherapy, Cancer, Regional Perfusion
Chemoreceptor Cells
3201.01 Oncología
3205.04 Hematología
quimioterapia del cáncer por perfusión regional
células quimiorreceptoras
síntomas de cáncer
topic AML
Cancer
Chemoresistance
Chemotherapy
Tyrosine kinase inhibitor
Chemotherapy, Cancer, Regional Perfusion
Chemoreceptor Cells
3201.01 Oncología
3205.04 Hematología
quimioterapia del cáncer por perfusión regional
células quimiorreceptoras
síntomas de cáncer
description [EN]Chemoresistance often limits the success of the pharmacological treatment in acute myeloid leukemia (AML) patients. Although positive results have been obtained with tyrosine kinase inhibitors (TKIs), such as sorafenib, especially in patients with Fms-like tyrosine kinase 3 (FLT3)-positive AML, the success of chemotherapy is very heterogeneous.The sensitivity to sorafenib-induced cell death (MTT test and anexin V/7-AAD method) was evaluated in five different cell lines: MOLM-13, OCI-AML2, HL-60, HEL and K-562. The transportome was characterized by measuring mRNA using RT-qPCR. Drug uptake/efflux was determined by flow cytometry using specific substrates and inhibitors. The cytostatic response to sorafenib was: MOLM-13»OCI-AML2>HL- 60>HEL≈K-562. Regarding efflux pumps, MDR1 was highly expressed in HEL>K- 562≈MOLM-13, but not in OCI-AML2 and HL-60. BCRP and MPR3 expression was low in all cell lines, whereas MRP4 and MRP5 expression was from moderate to high. Flow cytometry studies demonstrated that MRP4, but not MRP5, was functional.
publishDate 2018
dc.date.none.fl_str_mv 2018
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10366/146674
url http://hdl.handle.net/10366/146674
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Impact Journals LLC
publisher.none.fl_str_mv Impact Journals LLC
dc.source.none.fl_str_mv reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca
instname:Universidad de Salamanca (USAL)
instname_str Universidad de Salamanca (USAL)
reponame_str GREDOS. Repositorio Institucional de la Universidad de Salamanca
collection GREDOS. Repositorio Institucional de la Universidad de Salamanca
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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