Crystallization and preliminary X-ray diffraction studies of the eukaryotic iron superoxide dismutase (FeSOD) from Vigna unguiculata

12 Pags.- 1 Tabl.- 2 Figs. The definitive version is available at: http://onlinelibrary.wiley.com/journal/10.1111/%28ISSN%291399-0047

Detalles Bibliográficos
Autores: Muñoz, Inés G., Morán, José F., Becana Ausejo, Manuel, Montoya Blanco, Guillermo
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2003
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/99334
Acceso en línea:http://hdl.handle.net/10261/99334
Access Level:acceso abierto
Palabra clave:Vigna unguiculata
Vigna
Eukaryota
Escherichia coli
Superoxide dismutase
id ES_a1ccf46e9eff400f0ecfcaba65bf2ed6
oai_identifier_str oai:digital.csic.es:10261/99334
network_acronym_str ES
network_name_str España
repository_id_str
spelling Crystallization and preliminary X-ray diffraction studies of the eukaryotic iron superoxide dismutase (FeSOD) from Vigna unguiculataMuñoz, Inés G.Morán, José F.Becana Ausejo, ManuelMontoya Blanco, GuillermoVigna unguiculataVignaEukaryotaEscherichia coliSuperoxide dismutase12 Pags.- 1 Tabl.- 2 Figs. The definitive version is available at: http://onlinelibrary.wiley.com/journal/10.1111/%28ISSN%291399-0047Eukaryotic iron superoxide dismutases (FeSODs) are homodimeric proteins that constitute a fundamental protection against free radicals, which can damage essential cellular mechanisms. The protein was cloned and overexpressed in Escherichia coli with an N-terminal His tag. Crystallization experiments of the protein resulted, after several refined screenings, in crystals suitable for X-ray diffraction analysis. The crystals belong to the monoclinic space group C2, with unit-cell parameters a = 82.54, b = 48.41, c = 64.28 Å, α = γ = 90, β = 119.66°, and contain one molecule per asymmetric unit. At cryogenic temperatures, the crystals diffracted to a resolution limit of 1.80 Å using synchrotron radiation at the European Synchrotron Radiation Facility (ESRF).IGM thanks CNIO for a postdoctoral fellowship. JFM thanks CSIC-EU for an I3P postdoctoral contract. Full financial support was obtained through a CNIO internal grant to GM and partial support by grant PB-0522 (DGIC) to MB.Peer ReviewedBlackwell Publishing2014201420032014info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/99334reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1107/S0907444903006966info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/993342026-05-22T06:33:51Z
dc.title.none.fl_str_mv Crystallization and preliminary X-ray diffraction studies of the eukaryotic iron superoxide dismutase (FeSOD) from Vigna unguiculata
title Crystallization and preliminary X-ray diffraction studies of the eukaryotic iron superoxide dismutase (FeSOD) from Vigna unguiculata
spellingShingle Crystallization and preliminary X-ray diffraction studies of the eukaryotic iron superoxide dismutase (FeSOD) from Vigna unguiculata
Muñoz, Inés G.
Vigna unguiculata
Vigna
Eukaryota
Escherichia coli
Superoxide dismutase
title_short Crystallization and preliminary X-ray diffraction studies of the eukaryotic iron superoxide dismutase (FeSOD) from Vigna unguiculata
title_full Crystallization and preliminary X-ray diffraction studies of the eukaryotic iron superoxide dismutase (FeSOD) from Vigna unguiculata
title_fullStr Crystallization and preliminary X-ray diffraction studies of the eukaryotic iron superoxide dismutase (FeSOD) from Vigna unguiculata
title_full_unstemmed Crystallization and preliminary X-ray diffraction studies of the eukaryotic iron superoxide dismutase (FeSOD) from Vigna unguiculata
title_sort Crystallization and preliminary X-ray diffraction studies of the eukaryotic iron superoxide dismutase (FeSOD) from Vigna unguiculata
dc.creator.none.fl_str_mv Muñoz, Inés G.
Morán, José F.
Becana Ausejo, Manuel
Montoya Blanco, Guillermo
author Muñoz, Inés G.
author_facet Muñoz, Inés G.
Morán, José F.
Becana Ausejo, Manuel
Montoya Blanco, Guillermo
author_role author
author2 Morán, José F.
Becana Ausejo, Manuel
Montoya Blanco, Guillermo
author2_role author
author
author
dc.subject.none.fl_str_mv Vigna unguiculata
Vigna
Eukaryota
Escherichia coli
Superoxide dismutase
topic Vigna unguiculata
Vigna
Eukaryota
Escherichia coli
Superoxide dismutase
description 12 Pags.- 1 Tabl.- 2 Figs. The definitive version is available at: http://onlinelibrary.wiley.com/journal/10.1111/%28ISSN%291399-0047
publishDate 2003
dc.date.none.fl_str_mv 2003
2014
2014
2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/99334
url http://hdl.handle.net/10261/99334
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1107/S0907444903006966
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Blackwell Publishing
publisher.none.fl_str_mv Blackwell Publishing
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869415209223847936
score 15.81155

Similares en LA Referencia