The expression of <i>VDACs</i> and <i>Bcl2</i> family genes in pituitary adenomas: clinical correlations and postsurgical outcomes

Introduction: Pituitary adenomas (PAs) are benign tumors with high prevalence and, occasionally, aggressive course. The tumorigenesis of these lesions is not completely understood at the molecular level. BAK1 and BAX proteins play fundamental roles in apoptosis and seem to interact with VDAC protein...

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Autores: Facundo, AN, Magalhaes, M, Nascimento, GC, Azulay, RS, Santos, RM, Freitas, LA, Nascimento, AGPAC, Rodrigues, VP, Santos, WC, Beckman, AMGS, Abreu, JMF, Silva, RP, Carneiro, EL, Neto, CPO, da Costa, RMG, Corcoy, R, Mato, E, Faria, MS
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)
Repositorio:r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
OAI Identifier:oai:iibsantpau.fundanetsuite.com:p18554
Acceso en línea:https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=18554
http://ddd.uab.cat/record/309030
Access Level:acceso abierto
Palabra clave:pituitary adenoma
VDAC
bax
BAK
apoptosis
tumor regrowth
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spelling The expression of <i>VDACs</i> and <i>Bcl2</i> family genes in pituitary adenomas: clinical correlations and postsurgical outcomesFacundo, ANMagalhaes, MNascimento, GCAzulay, RSSantos, RMFreitas, LANascimento, AGPACRodrigues, VPSantos, WCBeckman, AMGSAbreu, JMFSilva, RPCarneiro, ELNeto, CPOda Costa, RMGCorcoy, RMato, EFaria, MSpituitary adenomaVDACbaxBAKapoptosistumor regrowthIntroduction: Pituitary adenomas (PAs) are benign tumors with high prevalence and, occasionally, aggressive course. The tumorigenesis of these lesions is not completely understood at the molecular level. BAK1 and BAX proteins play fundamental roles in apoptosis and seem to interact with VDAC proteins, whose expressions have been markedly altered in cancer, impacting their prognosis. Objective to evaluate the gene expression of VDAC1, VDAC2, BAK1 and BAX and their association with clinical and imaging characteristics in PA. Methods: Clinical-epidemiological data were collected from 117 tumor samples from patients affected by PA. Invasiveness was assessed by the Knosp scale. Gene expression was examined by real-time PCR. Relative expression analysis was performed by 2<^>(-DDCt) method. Results: The sample was mainly composed of women (69/117 - 57.2%). Tumor subtypes observed were Non-Functioning (NF) (73/117 - 62.4%), Acromegaly (24/117 - 20.5%) and Cushing's Disease (CD) (20/117 - 17.1%). Compared to normal tissue, there was a significant reduction in VDAC1 expression in the Acromegaly (p=0.029) and NF (p=0.002) groups. BAX expression was lower in all groups (p <0.001; p=0.007; P =0.005). No difference was found in VDAC2 and BAK1 expression, compared to normal pituitary. Overexpression of VDAC2 occurred in PAs with post-surgical regrowth (p=0.042). A strongly negative correlation was observed in BAX and BAK1 expression in CD. Conclusion: The results indicated that downregulations of VDAC1 and BAX may be related to resistance to apoptosis. In contrast, overexpression of VDAC2 in regrowing PAs suggests an antiapoptotic role for this gene. In summary, the genes evaluated might be involved in the biopathology of PAs.FRONTIERS MEDIA SA2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=18554http://ddd.uab.cat/record/309030Frontiers in EndocrinologyISSN: 16642392reponame:r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pauinstname:Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)Inglésinfo:eu-repo/semantics/openAccessoai:iibsantpau.fundanetsuite.com:p185542026-06-14T12:41:47Z
dc.title.none.fl_str_mv The expression of <i>VDACs</i> and <i>Bcl2</i> family genes in pituitary adenomas: clinical correlations and postsurgical outcomes
title The expression of <i>VDACs</i> and <i>Bcl2</i> family genes in pituitary adenomas: clinical correlations and postsurgical outcomes
spellingShingle The expression of <i>VDACs</i> and <i>Bcl2</i> family genes in pituitary adenomas: clinical correlations and postsurgical outcomes
Facundo, AN
pituitary adenoma
VDAC
bax
BAK
apoptosis
tumor regrowth
title_short The expression of <i>VDACs</i> and <i>Bcl2</i> family genes in pituitary adenomas: clinical correlations and postsurgical outcomes
title_full The expression of <i>VDACs</i> and <i>Bcl2</i> family genes in pituitary adenomas: clinical correlations and postsurgical outcomes
title_fullStr The expression of <i>VDACs</i> and <i>Bcl2</i> family genes in pituitary adenomas: clinical correlations and postsurgical outcomes
title_full_unstemmed The expression of <i>VDACs</i> and <i>Bcl2</i> family genes in pituitary adenomas: clinical correlations and postsurgical outcomes
title_sort The expression of <i>VDACs</i> and <i>Bcl2</i> family genes in pituitary adenomas: clinical correlations and postsurgical outcomes
dc.creator.none.fl_str_mv Facundo, AN
Magalhaes, M
Nascimento, GC
Azulay, RS
Santos, RM
Freitas, LA
Nascimento, AGPAC
Rodrigues, VP
Santos, WC
Beckman, AMGS
Abreu, JMF
Silva, RP
Carneiro, EL
Neto, CPO
da Costa, RMG
Corcoy, R
Mato, E
Faria, MS
author Facundo, AN
author_facet Facundo, AN
Magalhaes, M
Nascimento, GC
Azulay, RS
Santos, RM
Freitas, LA
Nascimento, AGPAC
Rodrigues, VP
Santos, WC
Beckman, AMGS
Abreu, JMF
Silva, RP
Carneiro, EL
Neto, CPO
da Costa, RMG
Corcoy, R
Mato, E
Faria, MS
author_role author
author2 Magalhaes, M
Nascimento, GC
Azulay, RS
Santos, RM
Freitas, LA
Nascimento, AGPAC
Rodrigues, VP
Santos, WC
Beckman, AMGS
Abreu, JMF
Silva, RP
Carneiro, EL
Neto, CPO
da Costa, RMG
Corcoy, R
Mato, E
Faria, MS
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv pituitary adenoma
VDAC
bax
BAK
apoptosis
tumor regrowth
topic pituitary adenoma
VDAC
bax
BAK
apoptosis
tumor regrowth
description Introduction: Pituitary adenomas (PAs) are benign tumors with high prevalence and, occasionally, aggressive course. The tumorigenesis of these lesions is not completely understood at the molecular level. BAK1 and BAX proteins play fundamental roles in apoptosis and seem to interact with VDAC proteins, whose expressions have been markedly altered in cancer, impacting their prognosis. Objective to evaluate the gene expression of VDAC1, VDAC2, BAK1 and BAX and their association with clinical and imaging characteristics in PA. Methods: Clinical-epidemiological data were collected from 117 tumor samples from patients affected by PA. Invasiveness was assessed by the Knosp scale. Gene expression was examined by real-time PCR. Relative expression analysis was performed by 2<^>(-DDCt) method. Results: The sample was mainly composed of women (69/117 - 57.2%). Tumor subtypes observed were Non-Functioning (NF) (73/117 - 62.4%), Acromegaly (24/117 - 20.5%) and Cushing's Disease (CD) (20/117 - 17.1%). Compared to normal tissue, there was a significant reduction in VDAC1 expression in the Acromegaly (p=0.029) and NF (p=0.002) groups. BAX expression was lower in all groups (p <0.001; p=0.007; P =0.005). No difference was found in VDAC2 and BAK1 expression, compared to normal pituitary. Overexpression of VDAC2 occurred in PAs with post-surgical regrowth (p=0.042). A strongly negative correlation was observed in BAX and BAK1 expression in CD. Conclusion: The results indicated that downregulations of VDAC1 and BAX may be related to resistance to apoptosis. In contrast, overexpression of VDAC2 in regrowing PAs suggests an antiapoptotic role for this gene. In summary, the genes evaluated might be involved in the biopathology of PAs.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=18554
http://ddd.uab.cat/record/309030
url https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=18554
http://ddd.uab.cat/record/309030
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv FRONTIERS MEDIA SA
publisher.none.fl_str_mv FRONTIERS MEDIA SA
dc.source.none.fl_str_mv Frontiers in Endocrinology
ISSN: 16642392
reponame:r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
instname:Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)
instname_str Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)
reponame_str r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
collection r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
repository.name.fl_str_mv
repository.mail.fl_str_mv
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