Food peptides as inducers of CCK and GLP-1 secretion and GPCRs involved in enteroendocrine cell signalling

The strong effect of protein digestion products on gastrointestinal-released hormones is recognised. However, little is known about the specific peptide sequences able to induce gastrointestinal hormone secretion and the receptors involved. Our objective was to identify peptides able to induce the s...

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Detalles Bibliográficos
Autores: Santos-Hernández, Marta, Vivanco-Maroto, Santiaga María, Miralles, Beatriz, Recio, Isidra
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/284897
Acceso en línea:http://hdl.handle.net/10261/284897
Access Level:acceso abierto
Palabra clave:CCK
GLP-1
Structure–activity relationship
GPCRs
Peptide signalling
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spelling Food peptides as inducers of CCK and GLP-1 secretion and GPCRs involved in enteroendocrine cell signallingSantos-Hernández, MartaVivanco-Maroto, Santiaga MaríaMiralles, BeatrizRecio, IsidraCCKGLP-1Structure–activity relationshipGPCRsPeptide signallingThe strong effect of protein digestion products on gastrointestinal-released hormones is recognised. However, little is known about the specific peptide sequences able to induce gastrointestinal hormone secretion and the receptors involved. Our objective was to identify peptides able to induce the secretion of cholecystokinin (CCK) and glucagon like peptide-1 (GLP-1) in the enteroendocrine cell line STC-1, and to evaluate the involvement of the calcium-sensing receptor and G-protein coupled receptor-93 in this cell signalling. The key role of the amino acidic sequence on CCK and GLP-1 secretion is demonstrated. Removing Ser from the N-terminus of κ-casein 33SRYPS37, or the N-terminal Trp-Ile in lysozyme 123WIRGCRL129 decreased the secretion of both hormones. However, substituting Tyr by Ala in peptide αs1-CN 90RYLG93 enhanced the CCK secretion levels but not the GLP-1 ones. In addition, the involvement of CaSR and GPR93 was evidenced, but our results pointed to the contribution of additional receptors or transporters.This work has received financial support from project PID2019-107663RB-I00 funded by MCIN/AEI/10.13039/501100011033 from the Spanish Ministry of Science and Innovation (MICINN). M. S-H. was the recipient of FPU grant (FPU15-03616) from the Ministry of Education, Culture and Sports. S.M.V.M. was the recipient of a contract in the project PIE-CSIC 202070E069.Peer reviewedElsevierMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Ministerio de Educación, Cultura y Deporte (España)Consejo Superior de Investigaciones Científicas (España)Ministerio de Ciencia e Innovación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttp://hdl.handle.net/10261/284897reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-107663RB-I00The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1016/j.foodchem.2022.134225The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1016/j.foodchem.2022.134225https://doi.org/10.1016/j.foodchem.2022.134225Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2848972026-05-22T06:33:51Z
dc.title.none.fl_str_mv Food peptides as inducers of CCK and GLP-1 secretion and GPCRs involved in enteroendocrine cell signalling
title Food peptides as inducers of CCK and GLP-1 secretion and GPCRs involved in enteroendocrine cell signalling
spellingShingle Food peptides as inducers of CCK and GLP-1 secretion and GPCRs involved in enteroendocrine cell signalling
Santos-Hernández, Marta
CCK
GLP-1
Structure–activity relationship
GPCRs
Peptide signalling
title_short Food peptides as inducers of CCK and GLP-1 secretion and GPCRs involved in enteroendocrine cell signalling
title_full Food peptides as inducers of CCK and GLP-1 secretion and GPCRs involved in enteroendocrine cell signalling
title_fullStr Food peptides as inducers of CCK and GLP-1 secretion and GPCRs involved in enteroendocrine cell signalling
title_full_unstemmed Food peptides as inducers of CCK and GLP-1 secretion and GPCRs involved in enteroendocrine cell signalling
title_sort Food peptides as inducers of CCK and GLP-1 secretion and GPCRs involved in enteroendocrine cell signalling
dc.creator.none.fl_str_mv Santos-Hernández, Marta
Vivanco-Maroto, Santiaga María
Miralles, Beatriz
Recio, Isidra
author Santos-Hernández, Marta
author_facet Santos-Hernández, Marta
Vivanco-Maroto, Santiaga María
Miralles, Beatriz
Recio, Isidra
author_role author
author2 Vivanco-Maroto, Santiaga María
Miralles, Beatriz
Recio, Isidra
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Ministerio de Educación, Cultura y Deporte (España)
Consejo Superior de Investigaciones Científicas (España)
Ministerio de Ciencia e Innovación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv CCK
GLP-1
Structure–activity relationship
GPCRs
Peptide signalling
topic CCK
GLP-1
Structure–activity relationship
GPCRs
Peptide signalling
description The strong effect of protein digestion products on gastrointestinal-released hormones is recognised. However, little is known about the specific peptide sequences able to induce gastrointestinal hormone secretion and the receptors involved. Our objective was to identify peptides able to induce the secretion of cholecystokinin (CCK) and glucagon like peptide-1 (GLP-1) in the enteroendocrine cell line STC-1, and to evaluate the involvement of the calcium-sensing receptor and G-protein coupled receptor-93 in this cell signalling. The key role of the amino acidic sequence on CCK and GLP-1 secretion is demonstrated. Removing Ser from the N-terminus of κ-casein 33SRYPS37, or the N-terminal Trp-Ile in lysozyme 123WIRGCRL129 decreased the secretion of both hormones. However, substituting Tyr by Ala in peptide αs1-CN 90RYLG93 enhanced the CCK secretion levels but not the GLP-1 ones. In addition, the involvement of CaSR and GPR93 was evidenced, but our results pointed to the contribution of additional receptors or transporters.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/284897
url http://hdl.handle.net/10261/284897
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-107663RB-I00
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1016/j.foodchem.2022.134225
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1016/j.foodchem.2022.134225
https://doi.org/10.1016/j.foodchem.2022.134225
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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