In vitro screening and lipid-lowering effect of prickly pear (Opuntia ficus-indica L. Mill.) fruit extracts in 3T3-L1 pre-adipocytes and mature adipocytes

Opuntia ficus-indica fruits have been widely used due to their nutritional composition and beneficial effects on health, particularly against chronic diseases such as diabetes, obesity, cardiovascular diseases and cancer, among others. In recent years, prickly pear peel and pulp extracts have been c...

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Autores: Eseberri, Itziar, Gómez-Maqueo, Andrea, Trepiana, Jenifer, Gómez-López, Iván, Proença, Carina, Cano, M. Pilar, Portillo, María P.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/359044
Acceso en línea:http://hdl.handle.net/10261/359044
Access Level:acceso abierto
Palabra clave:3T3-L1 adipocytes
Betalains
Isorhamnetin glycosides
Opuntia ficus-indica
Piscidic acid
Prickly pear
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spelling In vitro screening and lipid-lowering effect of prickly pear (Opuntia ficus-indica L. Mill.) fruit extracts in 3T3-L1 pre-adipocytes and mature adipocytesEseberri, ItziarGómez-Maqueo, AndreaTrepiana, JeniferGómez-López, IvánProença, CarinaCano, M. PilarPortillo, María P.3T3-L1 adipocytesBetalainsIsorhamnetin glycosidesOpuntia ficus-indicaPiscidic acidPrickly pearOpuntia ficus-indica fruits have been widely used due to their nutritional composition and beneficial effects on health, particularly against chronic diseases such as diabetes, obesity, cardiovascular diseases and cancer, among others. In recent years, prickly pear peel and pulp extracts have been characterised, and a high number of bioactive compounds have been identified. This study aimed to analyse the triglyceride-lowering effect of prickly pear peel and pulp extracts obtained from fruits of three varieties (Pelota, Sanguinos, and Colorada) in 3T3-L1 maturing and mature adipocytes. At a concentration of 50 µg/mL, peel extracts from Colorada reduced triglyceride accumulation in pre-adipocytes and mature adipocytes. Additionally, at 25 µg/mL, Pelota peel extract decreased triglyceride content in mature adipocytes. Moreover, maturing pre-adipocytes treated with 50 and 25 µg/mL of Sanguinos pulp extract showed a reduction of triglyceride accumulation. In addition, the lipid-lowering effect of the main individual betalain and phenolic compounds standards were assayed. Piscidic acid and isorhamnetin glycoside (IG2), found in Colorada peel extract, were identified as the bioactive compounds that could contribute more notably to the triglyceride-lowering effect of the extract. Thus, the betalain and phenolic-rich extracts from Opuntia ficus indica fruits may serve as an effective tool in obesity management.This study was supported by Ministerio de Ciencia e Innovación (PID2020-118300RB-C21 and PID2020-118300RB-C22 funded by MCIN/AEI/ https://doi.org/10.13039/501100011033 and by “ERDF A way of making Europe”, Instituto de Salud Carlos III (CIBERobn) (CB12/03/30007) and Government of the Basque Country (IT1482-22). Andrea Gómez-Maqueo acknowledges CONACyT (Mexico) for her doctoral scholarship no. 692751. Carina Proença acknowledges financial support from the project EXPL/MED-QUI/0815/2021.Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature.Peer reviewedSpringer NatureConferencia de Rectores de las Universidades EspañolasConsejo Superior de Investigaciones Científicas (España)Ministerio de Ciencia e Innovación (España)Agencia Estatal de Investigación (España)Ministerio de Ciencia, Innovación y Universidades (España)Instituto de Salud Carlos IIIEuropean CommissionEusko JaurlaritzaConsejo Nacional de Ciencia y Tecnología (México)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/359044reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-118300RB-C22info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-118300RB-C21The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1007/s11130-023-01137-8https://doi.org/10.1007/s11130-023-01137-8Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3590442026-05-22T06:33:51Z
dc.title.none.fl_str_mv In vitro screening and lipid-lowering effect of prickly pear (Opuntia ficus-indica L. Mill.) fruit extracts in 3T3-L1 pre-adipocytes and mature adipocytes
title In vitro screening and lipid-lowering effect of prickly pear (Opuntia ficus-indica L. Mill.) fruit extracts in 3T3-L1 pre-adipocytes and mature adipocytes
spellingShingle In vitro screening and lipid-lowering effect of prickly pear (Opuntia ficus-indica L. Mill.) fruit extracts in 3T3-L1 pre-adipocytes and mature adipocytes
Eseberri, Itziar
3T3-L1 adipocytes
Betalains
Isorhamnetin glycosides
Opuntia ficus-indica
Piscidic acid
Prickly pear
title_short In vitro screening and lipid-lowering effect of prickly pear (Opuntia ficus-indica L. Mill.) fruit extracts in 3T3-L1 pre-adipocytes and mature adipocytes
title_full In vitro screening and lipid-lowering effect of prickly pear (Opuntia ficus-indica L. Mill.) fruit extracts in 3T3-L1 pre-adipocytes and mature adipocytes
title_fullStr In vitro screening and lipid-lowering effect of prickly pear (Opuntia ficus-indica L. Mill.) fruit extracts in 3T3-L1 pre-adipocytes and mature adipocytes
title_full_unstemmed In vitro screening and lipid-lowering effect of prickly pear (Opuntia ficus-indica L. Mill.) fruit extracts in 3T3-L1 pre-adipocytes and mature adipocytes
title_sort In vitro screening and lipid-lowering effect of prickly pear (Opuntia ficus-indica L. Mill.) fruit extracts in 3T3-L1 pre-adipocytes and mature adipocytes
dc.creator.none.fl_str_mv Eseberri, Itziar
Gómez-Maqueo, Andrea
Trepiana, Jenifer
Gómez-López, Iván
Proença, Carina
Cano, M. Pilar
Portillo, María P.
author Eseberri, Itziar
author_facet Eseberri, Itziar
Gómez-Maqueo, Andrea
Trepiana, Jenifer
Gómez-López, Iván
Proença, Carina
Cano, M. Pilar
Portillo, María P.
author_role author
author2 Gómez-Maqueo, Andrea
Trepiana, Jenifer
Gómez-López, Iván
Proença, Carina
Cano, M. Pilar
Portillo, María P.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Conferencia de Rectores de las Universidades Españolas
Consejo Superior de Investigaciones Científicas (España)
Ministerio de Ciencia e Innovación (España)
Agencia Estatal de Investigación (España)
Ministerio de Ciencia, Innovación y Universidades (España)
Instituto de Salud Carlos III
European Commission
Eusko Jaurlaritza
Consejo Nacional de Ciencia y Tecnología (México)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv 3T3-L1 adipocytes
Betalains
Isorhamnetin glycosides
Opuntia ficus-indica
Piscidic acid
Prickly pear
topic 3T3-L1 adipocytes
Betalains
Isorhamnetin glycosides
Opuntia ficus-indica
Piscidic acid
Prickly pear
description Opuntia ficus-indica fruits have been widely used due to their nutritional composition and beneficial effects on health, particularly against chronic diseases such as diabetes, obesity, cardiovascular diseases and cancer, among others. In recent years, prickly pear peel and pulp extracts have been characterised, and a high number of bioactive compounds have been identified. This study aimed to analyse the triglyceride-lowering effect of prickly pear peel and pulp extracts obtained from fruits of three varieties (Pelota, Sanguinos, and Colorada) in 3T3-L1 maturing and mature adipocytes. At a concentration of 50 µg/mL, peel extracts from Colorada reduced triglyceride accumulation in pre-adipocytes and mature adipocytes. Additionally, at 25 µg/mL, Pelota peel extract decreased triglyceride content in mature adipocytes. Moreover, maturing pre-adipocytes treated with 50 and 25 µg/mL of Sanguinos pulp extract showed a reduction of triglyceride accumulation. In addition, the lipid-lowering effect of the main individual betalain and phenolic compounds standards were assayed. Piscidic acid and isorhamnetin glycoside (IG2), found in Colorada peel extract, were identified as the bioactive compounds that could contribute more notably to the triglyceride-lowering effect of the extract. Thus, the betalain and phenolic-rich extracts from Opuntia ficus indica fruits may serve as an effective tool in obesity management.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/359044
url http://hdl.handle.net/10261/359044
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-118300RB-C22
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-118300RB-C21
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1007/s11130-023-01137-8
https://doi.org/10.1007/s11130-023-01137-8
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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