Expression of adrenomedullin and proadrenomedullin N-terminal 20 peptide in human and rat prostate

Jimenez, N. (Nuria)|||/items/f30692dd-977b-4208-8cdd-35ce1f668019; Calvo-González, A. (Alfonso)|||/items/ac176653-87d5-476b-b2b9-ade455cfa6de; Rosell, D. (David)|||/items/4885ace6-0f1d-4ebf-b1cc-1ab79f2ea368; Cuttitta, F. (Frank)|||/items/0f0da08c-0efe-4d3c-97ca-44c38a26e188; Montuenga-Badia, L.M. (Luis M.)|||/items/4c999705-b2c9-45ac-ba13-3f18594ae596; Martinez, A. (Alfredo)|||/items/ce27c09e-454d-4645-986e-713760a3730d

Expression of adrenomedullin and proadrenomedullin N-terminal 20 peptide in human and rat prostate

Adrenomedullin (AM) and proadrenomedullin N-terminal 20 peptide (PAMP) are two recently discovered hypotensive peptides translated from the same message transcript (preproAM mRNA). In this article we report the presence of AM, PAMP, and their mRNA in human and rat prostate and of AM receptor mRNA in...

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Detalles Bibliográficos
Autores:	Jimenez, N. (Nuria)\|\|\|/items/f30692dd-977b-4208-8cdd-35ce1f668019, Calvo-González, A. (Alfonso)\|\|\|/items/ac176653-87d5-476b-b2b9-ade455cfa6de, Rosell, D. (David)\|\|\|/items/4885ace6-0f1d-4ebf-b1cc-1ab79f2ea368, Cuttitta, F. (Frank)\|\|\|/items/0f0da08c-0efe-4d3c-97ca-44c38a26e188, Montuenga-Badia, L.M. (Luis M.)\|\|\|/items/4c999705-b2c9-45ac-ba13-3f18594ae596, Martinez, A. (Alfredo)\|\|\|/items/ce27c09e-454d-4645-986e-713760a3730d
Tipo de recurso:	artículo
Fecha de publicación:	1999
País:	España
Institución:	Universidad de Navarra
Repositorio:	Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:	inglés
OAI Identifier:	oai:dadun.unav.edu:10171/20168
Acceso en línea:	https://hdl.handle.net/10171/20168
Access Level:	acceso abierto
Palabra clave:	Adrenomedullin Proadrenomedullin N-terminal 20 peptide Adrenomedullin receptor Prostate In situ hybridization RT-PCR Western blot

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spelling	Expression of adrenomedullin and proadrenomedullin N-terminal 20 peptide in human and rat prostateJimenez, N. (Nuria)\|\|\|/items/f30692dd-977b-4208-8cdd-35ce1f668019Calvo-González, A. (Alfonso)\|\|\|/items/ac176653-87d5-476b-b2b9-ade455cfa6deRosell, D. (David)\|\|\|/items/4885ace6-0f1d-4ebf-b1cc-1ab79f2ea368Cuttitta, F. (Frank)\|\|\|/items/0f0da08c-0efe-4d3c-97ca-44c38a26e188Montuenga-Badia, L.M. (Luis M.)\|\|\|/items/4c999705-b2c9-45ac-ba13-3f18594ae596Martinez, A. (Alfredo)\|\|\|/items/ce27c09e-454d-4645-986e-713760a3730dAdrenomedullinProadrenomedullin N-terminal 20 peptideAdrenomedullin receptorProstateIn situ hybridizationRT-PCRWestern blotAdrenomedullin (AM) and proadrenomedullin N-terminal 20 peptide (PAMP) are two recently discovered hypotensive peptides translated from the same message transcript (preproAM mRNA). In this article we report the presence of AM, PAMP, and their mRNA in human and rat prostate and of AM receptor mRNA in rat prostate. PreproAM mRNA was found in the epithelium of normal human and rat prostate glands by in situ hybridization. In humans, it was mainly expressed in the basal cells. In rat, its expression was higher in the ducts than in the acini of all the prostate lobes. Immunocytochemistry identified a similar distribution pattern for AM compared with its mRNA but showed different locations for AM and PAMP immunoreactivity. The former was widespread in the epithelia, whereas the latter was almost exclusively found in neuroendocrine cells. In rat, Western blot analysis confirmed the presence of high levels of AM peptide in the ventral lobe and of its precursor in the ventral and dorsolateral lobes. Immunoreactivity for serotonin, chromogranin A, PAMP, and AM defined four subpopulations of prostate neuroendocrine-like cells in rat, a cell type that has not been previously described.Histochemical SocietyDadun. Depósito Académico Digital Universidad de Navarra20112011-12-0919991999-01-0119991999-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/20168reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/201682026-06-21T12:47:57Z
dc.title.none.fl_str_mv	Expression of adrenomedullin and proadrenomedullin N-terminal 20 peptide in human and rat prostate
title	Expression of adrenomedullin and proadrenomedullin N-terminal 20 peptide in human and rat prostate
spellingShingle	Expression of adrenomedullin and proadrenomedullin N-terminal 20 peptide in human and rat prostate Jimenez, N. (Nuria)\|\|\|/items/f30692dd-977b-4208-8cdd-35ce1f668019 Adrenomedullin Proadrenomedullin N-terminal 20 peptide Adrenomedullin receptor Prostate In situ hybridization RT-PCR Western blot
title_short	Expression of adrenomedullin and proadrenomedullin N-terminal 20 peptide in human and rat prostate
title_full	Expression of adrenomedullin and proadrenomedullin N-terminal 20 peptide in human and rat prostate
title_fullStr	Expression of adrenomedullin and proadrenomedullin N-terminal 20 peptide in human and rat prostate
title_full_unstemmed	Expression of adrenomedullin and proadrenomedullin N-terminal 20 peptide in human and rat prostate
title_sort	Expression of adrenomedullin and proadrenomedullin N-terminal 20 peptide in human and rat prostate
dc.creator.none.fl_str_mv	Jimenez, N. (Nuria)\|\|\|/items/f30692dd-977b-4208-8cdd-35ce1f668019 Calvo-González, A. (Alfonso)\|\|\|/items/ac176653-87d5-476b-b2b9-ade455cfa6de Rosell, D. (David)\|\|\|/items/4885ace6-0f1d-4ebf-b1cc-1ab79f2ea368 Cuttitta, F. (Frank)\|\|\|/items/0f0da08c-0efe-4d3c-97ca-44c38a26e188 Montuenga-Badia, L.M. (Luis M.)\|\|\|/items/4c999705-b2c9-45ac-ba13-3f18594ae596 Martinez, A. (Alfredo)\|\|\|/items/ce27c09e-454d-4645-986e-713760a3730d
author	Jimenez, N. (Nuria)\|\|\|/items/f30692dd-977b-4208-8cdd-35ce1f668019
author_facet	Jimenez, N. (Nuria)\|\|\|/items/f30692dd-977b-4208-8cdd-35ce1f668019 Calvo-González, A. (Alfonso)\|\|\|/items/ac176653-87d5-476b-b2b9-ade455cfa6de Rosell, D. (David)\|\|\|/items/4885ace6-0f1d-4ebf-b1cc-1ab79f2ea368 Cuttitta, F. (Frank)\|\|\|/items/0f0da08c-0efe-4d3c-97ca-44c38a26e188 Montuenga-Badia, L.M. (Luis M.)\|\|\|/items/4c999705-b2c9-45ac-ba13-3f18594ae596 Martinez, A. (Alfredo)\|\|\|/items/ce27c09e-454d-4645-986e-713760a3730d
author_role	author
author2	Calvo-González, A. (Alfonso)\|\|\|/items/ac176653-87d5-476b-b2b9-ade455cfa6de Rosell, D. (David)\|\|\|/items/4885ace6-0f1d-4ebf-b1cc-1ab79f2ea368 Cuttitta, F. (Frank)\|\|\|/items/0f0da08c-0efe-4d3c-97ca-44c38a26e188 Montuenga-Badia, L.M. (Luis M.)\|\|\|/items/4c999705-b2c9-45ac-ba13-3f18594ae596 Martinez, A. (Alfredo)\|\|\|/items/ce27c09e-454d-4645-986e-713760a3730d
author2_role	author author author author author
dc.contributor.none.fl_str_mv	Dadun. Depósito Académico Digital Universidad de Navarra
dc.subject.none.fl_str_mv	Adrenomedullin Proadrenomedullin N-terminal 20 peptide Adrenomedullin receptor Prostate In situ hybridization RT-PCR Western blot
topic	Adrenomedullin Proadrenomedullin N-terminal 20 peptide Adrenomedullin receptor Prostate In situ hybridization RT-PCR Western blot
description	Adrenomedullin (AM) and proadrenomedullin N-terminal 20 peptide (PAMP) are two recently discovered hypotensive peptides translated from the same message transcript (preproAM mRNA). In this article we report the presence of AM, PAMP, and their mRNA in human and rat prostate and of AM receptor mRNA in rat prostate. PreproAM mRNA was found in the epithelium of normal human and rat prostate glands by in situ hybridization. In humans, it was mainly expressed in the basal cells. In rat, its expression was higher in the ducts than in the acini of all the prostate lobes. Immunocytochemistry identified a similar distribution pattern for AM compared with its mRNA but showed different locations for AM and PAMP immunoreactivity. The former was widespread in the epithelia, whereas the latter was almost exclusively found in neuroendocrine cells. In rat, Western blot analysis confirmed the presence of high levels of AM peptide in the ventral lobe and of its precursor in the ventral and dorsolateral lobes. Immunoreactivity for serotonin, chromogranin A, PAMP, and AM defined four subpopulations of prostate neuroendocrine-like cells in rat, a cell type that has not been previously described.
publishDate	1999
dc.date.none.fl_str_mv	1999 1999-01-01 1999 1999-01-01 2011 2011-12-09
dc.type.none.fl_str_mv	journal article http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	https://hdl.handle.net/10171/20168
url	https://hdl.handle.net/10171/20168
dc.language.none.fl_str_mv	Inglés eng
language_invalid_str_mv	Inglés
language	eng
dc.rights.none.fl_str_mv	open access http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv	info:eu-repo/semantics/openAccess
rights_invalid_str_mv	open access http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.publisher.none.fl_str_mv	Histochemical Society
publisher.none.fl_str_mv	Histochemical Society
dc.source.none.fl_str_mv	reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra instname:Universidad de Navarra
instname_str	Universidad de Navarra
reponame_str	Dadun. Depósito Académico Digital de la Universidad de Navarra
collection	Dadun. Depósito Académico Digital de la Universidad de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score	15.300719

Expression of adrenomedullin and proadrenomedullin N-terminal 20 peptide in human and rat prostate

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