Proteoliposomal formulations of an HIV-1 gp41-based miniprotein elicit a lipid-dependent immunodominant response overlapping the 2F5 binding motif

The HIV-1 gp41 Membrane Proximal External Region (MPER) is recognized by broadly neutralizing antibodies and represents a promising vaccine target. However, MPER immunogenicity and antibody activity are influenced by membrane lipids. To evaluate lipid modulation of MPER immunogenicity, we generated...

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Detalles Bibliográficos
Autores: Molinos-Albert, Luis Manuel|||0000-0001-5447-6803, Bilbao, Eneritz, Agulló, Luis, Marfil, Sílvia|||0000-0003-0139-5000, García Rodríguez, Elisabet, Rodriguez de la Concepción, Maria Luisa|||0000-0002-6944-3462, Izquierdo Useros, Nuria|||0000-0002-1039-1821, Vilaplana Massaguer, Cristina|||0000-0002-2808-7270, Nieto-Garai, Jon A., Contreras, F. Xabier, Floor, Martin, Cardona, Pere-Joan|||0000-0001-5623-7873, Martínez Picado, Francisco Javier|||0000-0002-4916-2129, Clotet Sala, Bonaventura|||0000-0003-3232-4598, Villà Freixa, Jordi|||0000-0002-6359-3929, Lorizate, Maier, Carrillo, Jorge|||0000-0003-0221-5948, Blanco, Julià|||0000-0002-2225-0217
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:196312
Acceso en línea:https://ddd.uab.cat/record/196312
https://dx.doi.org/urn:doi:10.1038/srep40800
Access Level:acceso abierto
Descripción
Sumario:The HIV-1 gp41 Membrane Proximal External Region (MPER) is recognized by broadly neutralizing antibodies and represents a promising vaccine target. However, MPER immunogenicity and antibody activity are influenced by membrane lipids. To evaluate lipid modulation of MPER immunogenicity, we generated a 1-Palmitoyl-2-oleoylphosphatidylcholine (POPC)-based proteoliposome collection containing combinations of phosphatidylserine (PS), GM3 ganglioside, holesterol (CHOL), sphingomyelin (SM) and the TLR4 agonist monophosphoryl lipid A (MPLA). A recombinant gp41-derived miniprotein (gp41-MinTT) exposing the MPER and a tetanus toxoid (TT) peptide that favors MHC-II presentation, was successfully incorporated into lipid mixtures (.

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