Natural killer cells improve hematopoietic stem cell engraftment by increasing stem cell clonogenicity in vitro and in a humanized mouse model

Cord blood (CB) is increasingly used as a source of hematopoietic stem cells (HSC) for transplantation. Low incidence and severity of graft-versus-host disease (GvHD) and a robust graft-versus-leukemia (GvL) effect are observed following CB transplantation (CBT). However, its main disadvantages are...

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Autores: Escobedo-Cousin, M., Jackson, N., Laza-Briviesca, Raquel, Ariza-McNaughton, L., Luevano, M., Derniame, S., Querol, Sergi|||0000-0002-1105-8633, Blundell, M., Thrasher, A., Soria, B., Cooper, N., Bonnet, Dominique, Madrigal, A., Saudemont, A.
Tipo de recurso: artículo
Fecha de publicación:2015
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:296546
Acceso en línea:https://ddd.uab.cat/record/296546
https://dx.doi.org/urn:doi:10.1371/journal.pone.0138623
Access Level:acceso abierto
Palabra clave:Animals
Cell Movement
Chemokine CXCL9
Cord Blood Stem Cell Transplantation
Cytokines
Female
Fetal Blood
Gene Expression Regulation
Graft vs Host Disease
Graft vs Leukemia Effect
Hematopoietic Stem Cell Transplantation
Hematopoietic Stem Cells
Humans
Interleukin-15
Killer Cells, Natural
Leukocytes, Mononuclear
Male
Mice
Mice, Inbred NOD
Mice, SCID
Oligonucleotide Array Sequence Analysis
Recombinant Proteins
Stem Cells
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spelling Natural killer cells improve hematopoietic stem cell engraftment by increasing stem cell clonogenicity in vitro and in a humanized mouse modelEscobedo-Cousin, M.Jackson, N.Laza-Briviesca, RaquelAriza-McNaughton, L.Luevano, M.Derniame, S.Querol, Sergi|||0000-0002-1105-8633Blundell, M.Thrasher, A.Soria, B.Cooper, N.Bonnet, DominiqueMadrigal, A.Saudemont, A.AnimalsCell MovementChemokine CXCL9Cord Blood Stem Cell TransplantationCytokinesFemaleFetal BloodGene Expression RegulationGraft vs Host DiseaseGraft vs Leukemia EffectHematopoietic Stem Cell TransplantationHematopoietic Stem CellsHumansInterleukin-15Killer Cells, NaturalLeukocytes, MononuclearMaleMiceMice, Inbred NODMice, SCIDOligonucleotide Array Sequence AnalysisRecombinant ProteinsStem CellsCord blood (CB) is increasingly used as a source of hematopoietic stem cells (HSC) for transplantation. Low incidence and severity of graft-versus-host disease (GvHD) and a robust graft-versus-leukemia (GvL) effect are observed following CB transplantation (CBT). However, its main disadvantages are a limited number of HSC per unit, delayed immune reconstitution and a higher incidence of infection. Unmanipulated grafts contain accessory cells that may facilitate HSC engraftment. Therefore, the effects of accessory cells, particularly natural killer (NK) cells, on human CB HSC (CBSC) functions were assessed in vitro and in vivo. CBSC cultured with autologous CB NK cells showed higher levels of CXCR4 expression, a higher migration index and a higher number of colony forming units (CFU) after short-term and long-term cultures. We found that CBSC secreted CXCL9 following interaction with CB NK cells. In addition, recombinant CXCL9 increased CBSC clonogenicity, recapitulating the effect observed of CB NK cells on CBSC. Moreover, the co-infusion of CBSC with CB NK cells led to a higher level of CBSC engraftment in NSG mouse model. The results presented in this work offer the basis for an alternative approach to enhance HSC engraftment that could improve the outcome of CBT. Copyright:.Universitat Autònoma de Barcelona 22015-01-0120152015-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/296546https://dx.doi.org/urn:doi:10.1371/journal.pone.0138623reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2965462026-06-06T12:50:31Z
dc.title.none.fl_str_mv Natural killer cells improve hematopoietic stem cell engraftment by increasing stem cell clonogenicity in vitro and in a humanized mouse model
title Natural killer cells improve hematopoietic stem cell engraftment by increasing stem cell clonogenicity in vitro and in a humanized mouse model
spellingShingle Natural killer cells improve hematopoietic stem cell engraftment by increasing stem cell clonogenicity in vitro and in a humanized mouse model
Escobedo-Cousin, M.
Animals
Cell Movement
Chemokine CXCL9
Cord Blood Stem Cell Transplantation
Cytokines
Female
Fetal Blood
Gene Expression Regulation
Graft vs Host Disease
Graft vs Leukemia Effect
Hematopoietic Stem Cell Transplantation
Hematopoietic Stem Cells
Humans
Interleukin-15
Killer Cells, Natural
Leukocytes, Mononuclear
Male
Mice
Mice, Inbred NOD
Mice, SCID
Oligonucleotide Array Sequence Analysis
Recombinant Proteins
Stem Cells
title_short Natural killer cells improve hematopoietic stem cell engraftment by increasing stem cell clonogenicity in vitro and in a humanized mouse model
title_full Natural killer cells improve hematopoietic stem cell engraftment by increasing stem cell clonogenicity in vitro and in a humanized mouse model
title_fullStr Natural killer cells improve hematopoietic stem cell engraftment by increasing stem cell clonogenicity in vitro and in a humanized mouse model
title_full_unstemmed Natural killer cells improve hematopoietic stem cell engraftment by increasing stem cell clonogenicity in vitro and in a humanized mouse model
title_sort Natural killer cells improve hematopoietic stem cell engraftment by increasing stem cell clonogenicity in vitro and in a humanized mouse model
dc.creator.none.fl_str_mv Escobedo-Cousin, M.
Jackson, N.
Laza-Briviesca, Raquel
Ariza-McNaughton, L.
Luevano, M.
Derniame, S.
Querol, Sergi|||0000-0002-1105-8633
Blundell, M.
Thrasher, A.
Soria, B.
Cooper, N.
Bonnet, Dominique
Madrigal, A.
Saudemont, A.
author Escobedo-Cousin, M.
author_facet Escobedo-Cousin, M.
Jackson, N.
Laza-Briviesca, Raquel
Ariza-McNaughton, L.
Luevano, M.
Derniame, S.
Querol, Sergi|||0000-0002-1105-8633
Blundell, M.
Thrasher, A.
Soria, B.
Cooper, N.
Bonnet, Dominique
Madrigal, A.
Saudemont, A.
author_role author
author2 Jackson, N.
Laza-Briviesca, Raquel
Ariza-McNaughton, L.
Luevano, M.
Derniame, S.
Querol, Sergi|||0000-0002-1105-8633
Blundell, M.
Thrasher, A.
Soria, B.
Cooper, N.
Bonnet, Dominique
Madrigal, A.
Saudemont, A.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universitat Autònoma de Barcelona
dc.subject.none.fl_str_mv Animals
Cell Movement
Chemokine CXCL9
Cord Blood Stem Cell Transplantation
Cytokines
Female
Fetal Blood
Gene Expression Regulation
Graft vs Host Disease
Graft vs Leukemia Effect
Hematopoietic Stem Cell Transplantation
Hematopoietic Stem Cells
Humans
Interleukin-15
Killer Cells, Natural
Leukocytes, Mononuclear
Male
Mice
Mice, Inbred NOD
Mice, SCID
Oligonucleotide Array Sequence Analysis
Recombinant Proteins
Stem Cells
topic Animals
Cell Movement
Chemokine CXCL9
Cord Blood Stem Cell Transplantation
Cytokines
Female
Fetal Blood
Gene Expression Regulation
Graft vs Host Disease
Graft vs Leukemia Effect
Hematopoietic Stem Cell Transplantation
Hematopoietic Stem Cells
Humans
Interleukin-15
Killer Cells, Natural
Leukocytes, Mononuclear
Male
Mice
Mice, Inbred NOD
Mice, SCID
Oligonucleotide Array Sequence Analysis
Recombinant Proteins
Stem Cells
description Cord blood (CB) is increasingly used as a source of hematopoietic stem cells (HSC) for transplantation. Low incidence and severity of graft-versus-host disease (GvHD) and a robust graft-versus-leukemia (GvL) effect are observed following CB transplantation (CBT). However, its main disadvantages are a limited number of HSC per unit, delayed immune reconstitution and a higher incidence of infection. Unmanipulated grafts contain accessory cells that may facilitate HSC engraftment. Therefore, the effects of accessory cells, particularly natural killer (NK) cells, on human CB HSC (CBSC) functions were assessed in vitro and in vivo. CBSC cultured with autologous CB NK cells showed higher levels of CXCR4 expression, a higher migration index and a higher number of colony forming units (CFU) after short-term and long-term cultures. We found that CBSC secreted CXCL9 following interaction with CB NK cells. In addition, recombinant CXCL9 increased CBSC clonogenicity, recapitulating the effect observed of CB NK cells on CBSC. Moreover, the co-infusion of CBSC with CB NK cells led to a higher level of CBSC engraftment in NSG mouse model. The results presented in this work offer the basis for an alternative approach to enhance HSC engraftment that could improve the outcome of CBT. Copyright:.
publishDate 2015
dc.date.none.fl_str_mv 2
2015-01-01
2015
2015-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/296546
https://dx.doi.org/urn:doi:10.1371/journal.pone.0138623
url https://ddd.uab.cat/record/296546
https://dx.doi.org/urn:doi:10.1371/journal.pone.0138623
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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