Production of (R)-3-hydroxybutyric acid from methane by in vivo depolymerization of polyhydroxybutyrate in Methylocystis parvus OBBP

Producción Científica

Detalles Bibliográficos
Autores: Yáñez, Luz, Rodríguez Muñoz, Yadira, Scott, Felipe, Vergara Fernández, Alberto, Muñoz Torre, Raúl
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Universidad de Valladolid
Repositorio:UVaDOC. Repositorio Documental de la Universidad de Valladolid
OAI Identifier:oai:uvadoc.uva.es:10324/52834
Acceso en línea:https://doi.org/10.1016/j.biortech.2022.127141
https://uvadoc.uva.es/handle/10324/52834
Access Level:acceso abierto
Palabra clave:Polyhydroxybutyrate
Polihidroxibutirato
Chiral compounds
Compuestos quirales
Methanotrophic bacteria
Metanotrofos
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spelling Production of (R)-3-hydroxybutyric acid from methane by in vivo depolymerization of polyhydroxybutyrate in Methylocystis parvus OBBPYáñez, LuzRodríguez Muñoz, YadiraScott, FelipeVergara Fernández, AlbertoMuñoz Torre, RaúlPolyhydroxybutyratePolihidroxibutiratoChiral compoundsCompuestos quiralesMethanotrophic bacteriaMetanotrofosProducción CientíficaMethylocystis parvus OBBP accumulates polyhydroxybutyrate (PHB) using methane as the sole carbon and energy source. In this work, the feasibility of producing (R)-3-hydroxybutyric acid (R3HBA) via intracellularly accumulated PHB through depolymerization (in-vivo) was investigated. Results showed that a PHB to R3HBA conversion of 77.2 ± 0.9% (R3HBA titer of 0.153 ± 0.002 g L-1) can be attained in a mineral medium containing 1 g L-1 KNO3 at 30 °C with shaking at 200 rpm and a constant pH of 11 for 72 h. Nitrogen deprivation and neutral or acidic pHs strongly reduced the excreted R3HBA concentration. Reduced oxygen availability negatively affected the R3HBA yield, which decreased to 73.6 ± 4.9% (titer of 0.139 ± 0.01 g L-1) under microaerobic conditions. Likewise, the presence of increasing concentrations of R3HBA in the medium before the onset of PHB depolymerization reduced the initial R3HBA release rate and R3HBA yield.Junta de Castilla y León - Fondo Europeo de Desarrollo Regional (projects CLU 2017-09 and UIC 315)Agencia Nacional de Investigación y Desarrollo de Chile (projects ANID/CONICYT Fondecyt Regular 1211434 and ANID/CONICYT Fondecyt Regular 1190521)Comisión Nacional de Investigación Científica y Tecnológica - PCI (grant REDES190137)Elsevier2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://doi.org/10.1016/j.biortech.2022.127141https://uvadoc.uva.es/handle/10324/52834reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolidinstname:Universidad de ValladolidIngléshttps://www.sciencedirect.com/science/article/pii/S0960852422004709?via%3Dihubinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/oai:uvadoc.uva.es:10324/528342026-06-13T12:44:47Z
dc.title.none.fl_str_mv Production of (R)-3-hydroxybutyric acid from methane by in vivo depolymerization of polyhydroxybutyrate in Methylocystis parvus OBBP
title Production of (R)-3-hydroxybutyric acid from methane by in vivo depolymerization of polyhydroxybutyrate in Methylocystis parvus OBBP
spellingShingle Production of (R)-3-hydroxybutyric acid from methane by in vivo depolymerization of polyhydroxybutyrate in Methylocystis parvus OBBP
Yáñez, Luz
Polyhydroxybutyrate
Polihidroxibutirato
Chiral compounds
Compuestos quirales
Methanotrophic bacteria
Metanotrofos
title_short Production of (R)-3-hydroxybutyric acid from methane by in vivo depolymerization of polyhydroxybutyrate in Methylocystis parvus OBBP
title_full Production of (R)-3-hydroxybutyric acid from methane by in vivo depolymerization of polyhydroxybutyrate in Methylocystis parvus OBBP
title_fullStr Production of (R)-3-hydroxybutyric acid from methane by in vivo depolymerization of polyhydroxybutyrate in Methylocystis parvus OBBP
title_full_unstemmed Production of (R)-3-hydroxybutyric acid from methane by in vivo depolymerization of polyhydroxybutyrate in Methylocystis parvus OBBP
title_sort Production of (R)-3-hydroxybutyric acid from methane by in vivo depolymerization of polyhydroxybutyrate in Methylocystis parvus OBBP
dc.creator.none.fl_str_mv Yáñez, Luz
Rodríguez Muñoz, Yadira
Scott, Felipe
Vergara Fernández, Alberto
Muñoz Torre, Raúl
author Yáñez, Luz
author_facet Yáñez, Luz
Rodríguez Muñoz, Yadira
Scott, Felipe
Vergara Fernández, Alberto
Muñoz Torre, Raúl
author_role author
author2 Rodríguez Muñoz, Yadira
Scott, Felipe
Vergara Fernández, Alberto
Muñoz Torre, Raúl
author2_role author
author
author
author
dc.subject.none.fl_str_mv Polyhydroxybutyrate
Polihidroxibutirato
Chiral compounds
Compuestos quirales
Methanotrophic bacteria
Metanotrofos
topic Polyhydroxybutyrate
Polihidroxibutirato
Chiral compounds
Compuestos quirales
Methanotrophic bacteria
Metanotrofos
description Producción Científica
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1016/j.biortech.2022.127141
https://uvadoc.uva.es/handle/10324/52834
url https://doi.org/10.1016/j.biortech.2022.127141
https://uvadoc.uva.es/handle/10324/52834
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0960852422004709?via%3Dihub
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolid
instname:Universidad de Valladolid
instname_str Universidad de Valladolid
reponame_str UVaDOC. Repositorio Documental de la Universidad de Valladolid
collection UVaDOC. Repositorio Documental de la Universidad de Valladolid
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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