Identification, typing and characterisation of Propionibacterium strains from healthy mucosa of the human stomach

Results were partially presented at the 3rd International Symposium on Propionibacteria and Bifidobacteria: Dairy and Probiotic Applications, held in June 2010, in Oviedo, Spain.

Detalles Bibliográficos
Autores: Delgado, Susana, Suárez, Adolfo, Mayo Pérez, Baltasar
Tipo de recurso: artículo
Fecha de publicación:2011
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/51469
Acceso en línea:http://hdl.handle.net/10261/51469
Access Level:acceso abierto
Palabra clave:Gastric microbiology
Human stomach
Propionibacteria
Propionibacteria acnes
Propionibacterium granulosum
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spelling Identification, typing and characterisation of Propionibacterium strains from healthy mucosa of the human stomachDelgado, SusanaSuárez, AdolfoMayo Pérez, BaltasarGastric microbiologyHuman stomachPropionibacteriaPropionibacteria acnesPropionibacterium granulosumResults were partially presented at the 3rd International Symposium on Propionibacteria and Bifidobacteria: Dairy and Probiotic Applications, held in June 2010, in Oviedo, Spain.Forty two Propionibacterium isolates were recovered from biopsy samples of the gastric mucosa of eight out of 12 healthy people. Of these, 41 were identified as belonging to Propionibacterium acnes; the remaining isolate was identified as belonging to Propionibacterium granulosum. Repetitive extragenic palindromic (REP)-PCR typing suggested that up to four strains might be present in the mucosa of the same individual. Sequence analysis of either recA, tly or camp5 genes of P. acnes isolates revealed two distinct phylogenetic lineages. As per the recA, most isolates belonged to type I, while the remainder of the isolates belonged to type II. Phenotypic analyses of representative isolates showed the different strains to have diverse biochemical properties. For example, large differences were seen in carbohydrate fermentation patterns, the results of qualitative and quantitative enzymatic profiling, and survival at acidic pH. In contrast, the patterns of resistance/susceptibility to a series of 16 antibiotics were rather similar, with no atypical resistances observed. The examined strains showed limited-if any-enzymatic activities that could be ultimately related to pathogenicity (lipolytic, proteolytic or haemolytic activity). This suggests that, in the gastric ecosystem, some Propionibacterium spp. genotypes and/or phenotypes can be considered true commensals. © 2011 Elsevier B.V.Research was supported by projects from FICYT (Ref. IB08-005) and INIA (Ref. RM2009-00009-00-00). S. Delgado was supported by a research contract from MICINN (Juan de la Cierva program, JCI-2008-02391).Peer ReviewedElsevierFundación para el Fomento en Asturias de la Investigación Científica Aplicada y la TecnologíaMinisterio de Ciencia e Innovación (España)2012201220112012info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/51469reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglésinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/514692026-05-22T06:33:51Z
dc.title.none.fl_str_mv Identification, typing and characterisation of Propionibacterium strains from healthy mucosa of the human stomach
title Identification, typing and characterisation of Propionibacterium strains from healthy mucosa of the human stomach
spellingShingle Identification, typing and characterisation of Propionibacterium strains from healthy mucosa of the human stomach
Delgado, Susana
Gastric microbiology
Human stomach
Propionibacteria
Propionibacteria acnes
Propionibacterium granulosum
title_short Identification, typing and characterisation of Propionibacterium strains from healthy mucosa of the human stomach
title_full Identification, typing and characterisation of Propionibacterium strains from healthy mucosa of the human stomach
title_fullStr Identification, typing and characterisation of Propionibacterium strains from healthy mucosa of the human stomach
title_full_unstemmed Identification, typing and characterisation of Propionibacterium strains from healthy mucosa of the human stomach
title_sort Identification, typing and characterisation of Propionibacterium strains from healthy mucosa of the human stomach
dc.creator.none.fl_str_mv Delgado, Susana
Suárez, Adolfo
Mayo Pérez, Baltasar
author Delgado, Susana
author_facet Delgado, Susana
Suárez, Adolfo
Mayo Pérez, Baltasar
author_role author
author2 Suárez, Adolfo
Mayo Pérez, Baltasar
author2_role author
author
dc.contributor.none.fl_str_mv Fundación para el Fomento en Asturias de la Investigación Científica Aplicada y la Tecnología
Ministerio de Ciencia e Innovación (España)
dc.subject.none.fl_str_mv Gastric microbiology
Human stomach
Propionibacteria
Propionibacteria acnes
Propionibacterium granulosum
topic Gastric microbiology
Human stomach
Propionibacteria
Propionibacteria acnes
Propionibacterium granulosum
description Results were partially presented at the 3rd International Symposium on Propionibacteria and Bifidobacteria: Dairy and Probiotic Applications, held in June 2010, in Oviedo, Spain.
publishDate 2011
dc.date.none.fl_str_mv 2011
2012
2012
2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/51469
url http://hdl.handle.net/10261/51469
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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