IgM-enriched immunoglobulin improves colistin efficacy in a pneumonia model by Pseudomonas aeruginosa
We evaluated the efficacy of ceftazidime or colistin in combi nation with polyclonal IgM-enriched immunoglobulin (IgM-IG), in an experimental pneumonia model (C57BL/6J male mice) using two multidrug-resistant Pseudomonas aeruginosa strains, both ceftazidime-susceptible and one colistin-resistant. Ph...
| Autores: | , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Universidad de Sevilla (US) |
| Repositorio: | idUS. Depósito de Investigación de la Universidad de Sevilla |
| OAI Identifier: | oai:idus.us.es:11441/145619 |
| Acceso en línea: | https://hdl.handle.net/11441/145619 https://doi.org/10.26508/lsa.202101349 |
| Access Level: | acceso abierto |
| Palabra clave: | Pseudomonas aeruginosa Immunoglobulin IgM Colistin Pneumonia model |
| Sumario: | We evaluated the efficacy of ceftazidime or colistin in combi nation with polyclonal IgM-enriched immunoglobulin (IgM-IG), in an experimental pneumonia model (C57BL/6J male mice) using two multidrug-resistant Pseudomonas aeruginosa strains, both ceftazidime-susceptible and one colistin-resistant. Pharmaco dynamically optimised antimicrobials were administered for 72 h, and intravenous IgM-IG was given as a single dose. Bacterial tissues count and the mortality were analysed. Ceftazidime was more effective than colistin for both strains. In mice infected with the colistin-susceptible strain, ceftazidime reduced the bacterial concentration in the lungs and blood (22.42 and 23.87 log10 CFU/ ml) compared with colistin (20.55 and 21.23 log10 CFU/ml, re spectively) and with the controls. Colistin plus IgM-IG reduced the bacterial lung concentrations of both colistin-susceptible and resistant strains (22.91 and 21.73 log10 CFU/g, respectively) and the bacteraemia rate of the colistin-resistant strain (244%). These results suggest that IgM-IG might be useful as an adjuvant to colistin in the treatment of pneumonia caused by multidrug resistant P. aeruginosa. |
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