REDOX Balance in Oligodendrocytes Is Important for Zebrafish Visual System Regeneration
[EN]Zebrafish (Danio rerio) present continuous growth and regenerate many parts of their body after an injury. Fish oligodendrocytes, microglia and astrocytes support the formation of new connections producing effective regeneration of the central nervous system after a lesion. To understand the rol...
| Autores: | , , , , , |
|---|---|
| Formato: | artículo |
| Fecha de publicación: | 2023 |
| País: | España |
| Recursos: | Universidad de Salamanca (USAL) |
| Repositorio: | GREDOS. Repositorio Institucional de la Universidad de Salamanca |
| OAI Identifier: | oai:gredos.usal.es:10366/155091 |
| Acesso em linha: | http://hdl.handle.net/10366/155091 |
| Access Level: | acceso abierto |
| Palavra-chave: | Oligodendrocytes Zebrafish Regeneration Visual System REDOX Neurosciences Nerve Regeneration 2490 Neurociencias regeneración nerviosa neurociencias |
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REDOX Balance in Oligodendrocytes Is Important for Zebrafish Visual System RegenerationPérez-Montes, CristinaJiménez-Cubides, Jhoana PaolaVelasco Arranz, María AlmudenaArévalo Arévalo, María RosarioSantos Ledo, AdriánGarcía Macia, MarinaOligodendrocytesZebrafishRegenerationVisual SystemREDOXNeurosciencesNerve Regeneration2490 Neurocienciasregeneración nerviosaneurociencias[EN]Zebrafish (Danio rerio) present continuous growth and regenerate many parts of their body after an injury. Fish oligodendrocytes, microglia and astrocytes support the formation of new connections producing effective regeneration of the central nervous system after a lesion. To understand the role of oligodendrocytes and the signals that mediate regeneration, we use the well-established optic nerve (ON) crush model. We also used sox10 fluorescent transgenic lines to label fully differentiated oligodendrocytes. To quench the effect of reactive oxygen species (ROS), we used the endogenous antioxidant melatonin. Using these tools, we measured ROS production by flow cytometry and explored the regeneration of the optic tectum (OT), the response of oligodendrocytes and their mitochondria by confocal microscopy and Western blot. ROS are produced by oligodendrocytes 3 h after injury and JNK activity is triggered. Concomitantly, there is a decrease in the number of fully differentiated oligodendrocytes in the OT and in their mitochondrial population. By 24 h, oligodendrocytes partially recover. Exposure to melatonin blocks the changes observed in these oligodendrocytes at 3 h and increases their number and their mitochondrial populations after 24 h. Melatonin also blocks JNK upregulation and induces aberrant neuronal differentiation in the OT. In conclusion, a proper balance of ROS is necessary during visual system regeneration and exposure to melatonin has a detrimental impact.his project was funded by: Lanzadera TCUE21-21_003, C2 program for Universidad de Salamanca; Convocatoria de ayudas de investigación Biomédica 2021, FERP21/003, Fundación Eugenio Rodríguez Pascual; ESCI 2022 Exploratory Research Grants; ESCI22/001, European Society for Clinical Investigation ESCI; Internationalization Project “CL-EI-2021-08-IBFG Unit of Excellence” from Spanish National Research Council (CSIC), funded by the Regional Government of Castile and Leon and co-financed by the European Regional Development Fund (ERDF “Europe drives our growth”). CPM is a USAL fellow Plan Especial Grado Medicina. MGM is a Ramón y Cajal Researcher RYC2021-033684-I, MICIN. We are members of Women in Autophagy (WIA), la Sociedad Española de Bioquímica y Biología Molecular (SEBBM) and la Sociedad Española de Autofagia (SEFAGIA).MDPI202420242023info:eu-repo/semantics/articlehttp://hdl.handle.net/10366/155091reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)InglésLanzadera TCUE21-21_003FERP21/003ESCI 2022 Exploratory Research GrantsESCI22/001, European Society for Clinical Investigation ESCIEI-2021-08-IBFG Unit of ExcellenceRYC2021-033684-IAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:gredos.usal.es:10366/1550912026-06-07T06:28:51Z |
| dc.title.none.fl_str_mv |
REDOX Balance in Oligodendrocytes Is Important for Zebrafish Visual System Regeneration |
| title |
REDOX Balance in Oligodendrocytes Is Important for Zebrafish Visual System Regeneration |
| spellingShingle |
REDOX Balance in Oligodendrocytes Is Important for Zebrafish Visual System Regeneration Pérez-Montes, Cristina Oligodendrocytes Zebrafish Regeneration Visual System REDOX Neurosciences Nerve Regeneration 2490 Neurociencias regeneración nerviosa neurociencias |
| title_short |
REDOX Balance in Oligodendrocytes Is Important for Zebrafish Visual System Regeneration |
| title_full |
REDOX Balance in Oligodendrocytes Is Important for Zebrafish Visual System Regeneration |
| title_fullStr |
REDOX Balance in Oligodendrocytes Is Important for Zebrafish Visual System Regeneration |
| title_full_unstemmed |
REDOX Balance in Oligodendrocytes Is Important for Zebrafish Visual System Regeneration |
| title_sort |
REDOX Balance in Oligodendrocytes Is Important for Zebrafish Visual System Regeneration |
| dc.creator.none.fl_str_mv |
Pérez-Montes, Cristina Jiménez-Cubides, Jhoana Paola Velasco Arranz, María Almudena Arévalo Arévalo, María Rosario Santos Ledo, Adrián García Macia, Marina |
| author |
Pérez-Montes, Cristina |
| author_facet |
Pérez-Montes, Cristina Jiménez-Cubides, Jhoana Paola Velasco Arranz, María Almudena Arévalo Arévalo, María Rosario Santos Ledo, Adrián García Macia, Marina |
| author_role |
author |
| author2 |
Jiménez-Cubides, Jhoana Paola Velasco Arranz, María Almudena Arévalo Arévalo, María Rosario Santos Ledo, Adrián García Macia, Marina |
| author2_role |
author author author author author |
| dc.subject.none.fl_str_mv |
Oligodendrocytes Zebrafish Regeneration Visual System REDOX Neurosciences Nerve Regeneration 2490 Neurociencias regeneración nerviosa neurociencias |
| topic |
Oligodendrocytes Zebrafish Regeneration Visual System REDOX Neurosciences Nerve Regeneration 2490 Neurociencias regeneración nerviosa neurociencias |
| description |
[EN]Zebrafish (Danio rerio) present continuous growth and regenerate many parts of their body after an injury. Fish oligodendrocytes, microglia and astrocytes support the formation of new connections producing effective regeneration of the central nervous system after a lesion. To understand the role of oligodendrocytes and the signals that mediate regeneration, we use the well-established optic nerve (ON) crush model. We also used sox10 fluorescent transgenic lines to label fully differentiated oligodendrocytes. To quench the effect of reactive oxygen species (ROS), we used the endogenous antioxidant melatonin. Using these tools, we measured ROS production by flow cytometry and explored the regeneration of the optic tectum (OT), the response of oligodendrocytes and their mitochondria by confocal microscopy and Western blot. ROS are produced by oligodendrocytes 3 h after injury and JNK activity is triggered. Concomitantly, there is a decrease in the number of fully differentiated oligodendrocytes in the OT and in their mitochondrial population. By 24 h, oligodendrocytes partially recover. Exposure to melatonin blocks the changes observed in these oligodendrocytes at 3 h and increases their number and their mitochondrial populations after 24 h. Melatonin also blocks JNK upregulation and induces aberrant neuronal differentiation in the OT. In conclusion, a proper balance of ROS is necessary during visual system regeneration and exposure to melatonin has a detrimental impact. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023 2024 2024 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10366/155091 |
| url |
http://hdl.handle.net/10366/155091 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Lanzadera TCUE21-21_003 FERP21/003 ESCI 2022 Exploratory Research Grants ESCI22/001, European Society for Clinical Investigation ESCI EI-2021-08-IBFG Unit of Excellence RYC2021-033684-I |
| dc.rights.none.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
MDPI |
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MDPI |
| dc.source.none.fl_str_mv |
reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca instname:Universidad de Salamanca (USAL) |
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Universidad de Salamanca (USAL) |
| reponame_str |
GREDOS. Repositorio Institucional de la Universidad de Salamanca |
| collection |
GREDOS. Repositorio Institucional de la Universidad de Salamanca |
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15,300719 |