Cardiovascular Risk Factors and Differential Transcriptomic Profile of the Subcutaneous and Visceral Adipose Tissue and Their Resident Stem Cells

Background: The increase in the incidence of obesity and obesity-related cardiovascular risk factors (CVRFs) over the last decades has brought attention on adipose tissue (AT) pathobiology. The expansion of AT is associated with the development of new vasculature needed to perfuse the tissue; howeve...

Descripción completa

Detalles Bibliográficos
Autores: Arderiu, Gemma|||0000-0001-8598-4106, Ballesta, Carlos, Moscatiello, Fabrizio, Vilahur, Gemma|||0000-0002-2828-8873, Badimon, Lina|||0000-0002-9162-2459, Lambert, Carmen|||0000-0002-7929-4990
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:233402
Acceso en línea:https://ddd.uab.cat/record/233402
https://dx.doi.org/urn:doi:10.3390/cells9102235
Access Level:acceso abierto
Palabra clave:Adipose stem cells
Endothelial cells
Microvesicles
Obesity
Cardiovascular risk factors
And angiogenesis
id ES_a04ca82a42e3cdd5414a3b3cbb38f7a6
oai_identifier_str oai:ddd.uab.cat:233402
network_acronym_str ES
network_name_str España
repository_id_str
spelling Cardiovascular Risk Factors and Differential Transcriptomic Profile of the Subcutaneous and Visceral Adipose Tissue and Their Resident Stem CellsArderiu, Gemma|||0000-0001-8598-4106Ballesta, CarlosMoscatiello, FabrizioVilahur, Gemma|||0000-0002-2828-8873Badimon, Lina|||0000-0002-9162-2459Lambert, Carmen|||0000-0002-7929-4990Adipose stem cellsEndothelial cellsMicrovesiclesObesityCardiovascular risk factorsAnd angiogenesisBackground: The increase in the incidence of obesity and obesity-related cardiovascular risk factors (CVRFs) over the last decades has brought attention on adipose tissue (AT) pathobiology. The expansion of AT is associated with the development of new vasculature needed to perfuse the tissue; however, not all fat depots have the same ability to induce angiogenesis that requires recruitment of their own endothelial cells. In this study we have investigated the effect of different CVRFs, on the angiogenic capacity of the subcutaneous (SAT) and visceral (VAT) adipose tissue and on the function of their mesenchymal cell reservoir. Methods: A transcriptomic approach was used to compare the different angiogenic and inflammatory profiles of the subcutaneous and visceral fat depots from individuals with obesity, as well as their resident stem cells (ASCs). Influence of other risk factors on fat composition was also measured. Finally, the microvesicles (MVs) released by ASCs were isolated and their regenerative potential analyzed by molecular and cellular methodologies. Results: Obesity decreases the angiogenic capacity of AT. There are differences between SAT and VAT; from the 21 angiogenic-related genes analyzed, only three were decreased in SAT compared with those decreased in VAT. ASCs isolated from both fat depots showed significant differences; there was a significant up-regulation of the VEGF-pathway on visceral derived ASCs. ASCs release MVs that stimulate endothelial cell migration and angiogenic capacity. Conclusions: In patients with obesity, SAT expresses a greater number of angiogenic molecules than VAT, independent of the presence of other CVRFs.Universitat Autònoma de Barcelona 22020-01-0120202020-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/233402https://dx.doi.org/urn:doi:10.3390/cells9102235reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 SAF2016-76819-RMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 TERCELRD16/0011/0018Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 CB16-11-0041Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 P17-01321Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-1480open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2334022026-06-06T12:50:31Z
dc.title.none.fl_str_mv Cardiovascular Risk Factors and Differential Transcriptomic Profile of the Subcutaneous and Visceral Adipose Tissue and Their Resident Stem Cells
title Cardiovascular Risk Factors and Differential Transcriptomic Profile of the Subcutaneous and Visceral Adipose Tissue and Their Resident Stem Cells
spellingShingle Cardiovascular Risk Factors and Differential Transcriptomic Profile of the Subcutaneous and Visceral Adipose Tissue and Their Resident Stem Cells
Arderiu, Gemma|||0000-0001-8598-4106
Adipose stem cells
Endothelial cells
Microvesicles
Obesity
Cardiovascular risk factors
And angiogenesis
title_short Cardiovascular Risk Factors and Differential Transcriptomic Profile of the Subcutaneous and Visceral Adipose Tissue and Their Resident Stem Cells
title_full Cardiovascular Risk Factors and Differential Transcriptomic Profile of the Subcutaneous and Visceral Adipose Tissue and Their Resident Stem Cells
title_fullStr Cardiovascular Risk Factors and Differential Transcriptomic Profile of the Subcutaneous and Visceral Adipose Tissue and Their Resident Stem Cells
title_full_unstemmed Cardiovascular Risk Factors and Differential Transcriptomic Profile of the Subcutaneous and Visceral Adipose Tissue and Their Resident Stem Cells
title_sort Cardiovascular Risk Factors and Differential Transcriptomic Profile of the Subcutaneous and Visceral Adipose Tissue and Their Resident Stem Cells
dc.creator.none.fl_str_mv Arderiu, Gemma|||0000-0001-8598-4106
Ballesta, Carlos
Moscatiello, Fabrizio
Vilahur, Gemma|||0000-0002-2828-8873
Badimon, Lina|||0000-0002-9162-2459
Lambert, Carmen|||0000-0002-7929-4990
author Arderiu, Gemma|||0000-0001-8598-4106
author_facet Arderiu, Gemma|||0000-0001-8598-4106
Ballesta, Carlos
Moscatiello, Fabrizio
Vilahur, Gemma|||0000-0002-2828-8873
Badimon, Lina|||0000-0002-9162-2459
Lambert, Carmen|||0000-0002-7929-4990
author_role author
author2 Ballesta, Carlos
Moscatiello, Fabrizio
Vilahur, Gemma|||0000-0002-2828-8873
Badimon, Lina|||0000-0002-9162-2459
Lambert, Carmen|||0000-0002-7929-4990
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universitat Autònoma de Barcelona
dc.subject.none.fl_str_mv Adipose stem cells
Endothelial cells
Microvesicles
Obesity
Cardiovascular risk factors
And angiogenesis
topic Adipose stem cells
Endothelial cells
Microvesicles
Obesity
Cardiovascular risk factors
And angiogenesis
description Background: The increase in the incidence of obesity and obesity-related cardiovascular risk factors (CVRFs) over the last decades has brought attention on adipose tissue (AT) pathobiology. The expansion of AT is associated with the development of new vasculature needed to perfuse the tissue; however, not all fat depots have the same ability to induce angiogenesis that requires recruitment of their own endothelial cells. In this study we have investigated the effect of different CVRFs, on the angiogenic capacity of the subcutaneous (SAT) and visceral (VAT) adipose tissue and on the function of their mesenchymal cell reservoir. Methods: A transcriptomic approach was used to compare the different angiogenic and inflammatory profiles of the subcutaneous and visceral fat depots from individuals with obesity, as well as their resident stem cells (ASCs). Influence of other risk factors on fat composition was also measured. Finally, the microvesicles (MVs) released by ASCs were isolated and their regenerative potential analyzed by molecular and cellular methodologies. Results: Obesity decreases the angiogenic capacity of AT. There are differences between SAT and VAT; from the 21 angiogenic-related genes analyzed, only three were decreased in SAT compared with those decreased in VAT. ASCs isolated from both fat depots showed significant differences; there was a significant up-regulation of the VEGF-pathway on visceral derived ASCs. ASCs release MVs that stimulate endothelial cell migration and angiogenic capacity. Conclusions: In patients with obesity, SAT expresses a greater number of angiogenic molecules than VAT, independent of the presence of other CVRFs.
publishDate 2020
dc.date.none.fl_str_mv 2
2020-01-01
2020
2020-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/233402
https://dx.doi.org/urn:doi:10.3390/cells9102235
url https://ddd.uab.cat/record/233402
https://dx.doi.org/urn:doi:10.3390/cells9102235
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 SAF2016-76819-R
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 TERCELRD16/0011/0018
Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 CB16-11-0041
Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 P17-01321
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-1480
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869414999511793664
score 15,300719

Similares en LA Referencia