Identification, biochemical composition and phycobiliproteins production of Chroococcidiopsis sp. from arid environment
Molecular and microscopic studies were performed to identify Chroococcidiopsis sp., an endolithic cyanobacterium, isolated from gypsum rocks of Atacama Desert (Chile). It was adapted to grow in mineral liquid medium, with 9mM nitrate, bubbled with CO2-enriched air (2.5 % v/v), and continuously illum...
| Autores: | , , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2020 |
| País: | España |
| Institución: | Universidad de Huelva (UHU) |
| Repositorio: | Arias Montano. Repositorio Institucional de la Universidad de Huelva |
| Idioma: | inglés |
| OAI Identifier: | oai:ariasmontano.uhu.es:10272/23588 |
| Acceso en línea: | https://hdl.handle.net/10272/23588 |
| Access Level: | acceso abierto |
| Palabra clave: | Allophycocyanin Atacama desert Chroococcidiopsis sp. Endolithic Phycobiliproteins 23 Química |
| Sumario: | Molecular and microscopic studies were performed to identify Chroococcidiopsis sp., an endolithic cyanobacterium, isolated from gypsum rocks of Atacama Desert (Chile). It was adapted to grow in mineral liquid medium, with 9mM nitrate, bubbled with CO2-enriched air (2.5 % v/v), and continuously illuminated with a white light of 70μmol photonsm–2s–1. The obtained biomass (productivity of 0.21gL–1d–1) had a C/N ratio of 6.67, and it contained carbohydrates (45.40 % of dry weight), proteins (36.72 %), lipids (5.60 %) nucleic acids (3.90 %) and ashes (8.28 %). The lipid fraction was particularly rich in palmitic (29.86 % of total fatty acids), linoleic (18.20 %), palmitoleic (12.75 %), linolenic (10.92 %), stearic (9.64 %) and capric acid (6.29 %). Chroococcidiopsis sp. accumulated phycobiliproteins in a light-dependent process and produced 204mgg–1, under incident light of 10 μmol photons·m–2·s–1, with a relative abundance of 40.9 % for phycocyanin, 23.3 % for phycoerythrin, and 35.8 % for allophycocyanin. The biomass from this cyanobacterium can be a good source of these pigments, especially APC (maximum of 95mgg dw−1), which are of interest for pharmacological, cosmetic, and food industries |
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