Characterization of the bHLH family of transcriptional regulators in the acoel S. roscofensis and their putative role in neurogenesis

Background: The basic helix-loop-helix (bHLH) family of transcription factors is one of the largest superfamilies of regulatory transcription factors and is widely used in eukaryotic organisms. They play an essential role in a range of metabolic, physiological, and developmental processes, including...

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Autores: Perea Atienza, Elena, Sprecher, Simon G., Martínez Serra, Pedro
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/122079
Acceso en línea:https://hdl.handle.net/2445/122079
Access Level:acceso abierto
Palabra clave:Neurobiologia
Embriologia
Transcripció genètica
Invertebrats
Neurobiology
Embryology
Genetic transcription
Invertebrates
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spelling Characterization of the bHLH family of transcriptional regulators in the acoel S. roscofensis and their putative role in neurogenesisPerea Atienza, ElenaSprecher, Simon G.Martínez Serra, PedroNeurobiologiaEmbriologiaTranscripció genèticaInvertebratsNeurobiologyEmbryologyGenetic transcriptionInvertebratesBackground: The basic helix-loop-helix (bHLH) family of transcription factors is one of the largest superfamilies of regulatory transcription factors and is widely used in eukaryotic organisms. They play an essential role in a range of metabolic, physiological, and developmental processes, including the development of the nervous system (NS). These transcription factors have been studied in many metazoans, especially in vertebrates but also in early branching metazoan clades such as the cnidarians and sponges. However, currently very little is known about their expression in the most basally branching bilaterian group, the xenacoelomorphs. Recently, our laboratory has characterized the full complement of bHLH in the genome of two members of the Xenacoelomorpha, the xenoturbellid Xenoturbella bocki and the acoel Symsagittifera roscoffensis. Understanding the patterns of bHLH gene expression in members of this phylum (in space and time) provides critical new insights into the conserved roles of the bHLH and their putative specificities in this group. Our focus is on deciphering the specific roles that these genes have in the process of neurogenesis. Results: Here, we analyze the developmental expression of the whole complement of bHLH genes identified in the acoel S. roscoffensis. Based on their expression patterns, several members of bHLH class A appear to have specific conserved roles in neurogenesis, while other class A genes (as well as members of other classes) have likely taken on more generalized functions. All gene expression patterns are described in embryos and early juveniles. Conclusion: Our results suggest that the main roles of the bHLH genes of S. roscoffensis are evolutionarily conserved, with a specific subset dedicated to patterning the nervous system: SrAscA, SrAscB, SrHes/Hey, SrNscl, SrSrebp, SrE12/ E47 and SrOlig.2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/122079Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1186/s13227-018-0097-yEvodevo, 2018, vol. 9, num. 8https://doi.org/10.1186/s13227-018-0097-ycc-by (c) Perea-Atienza, E. et al., 2018http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1220792026-05-27T06:46:51Z
dc.title.none.fl_str_mv Characterization of the bHLH family of transcriptional regulators in the acoel S. roscofensis and their putative role in neurogenesis
title Characterization of the bHLH family of transcriptional regulators in the acoel S. roscofensis and their putative role in neurogenesis
spellingShingle Characterization of the bHLH family of transcriptional regulators in the acoel S. roscofensis and their putative role in neurogenesis
Perea Atienza, Elena
Neurobiologia
Embriologia
Transcripció genètica
Invertebrats
Neurobiology
Embryology
Genetic transcription
Invertebrates
title_short Characterization of the bHLH family of transcriptional regulators in the acoel S. roscofensis and their putative role in neurogenesis
title_full Characterization of the bHLH family of transcriptional regulators in the acoel S. roscofensis and their putative role in neurogenesis
title_fullStr Characterization of the bHLH family of transcriptional regulators in the acoel S. roscofensis and their putative role in neurogenesis
title_full_unstemmed Characterization of the bHLH family of transcriptional regulators in the acoel S. roscofensis and their putative role in neurogenesis
title_sort Characterization of the bHLH family of transcriptional regulators in the acoel S. roscofensis and their putative role in neurogenesis
dc.creator.none.fl_str_mv Perea Atienza, Elena
Sprecher, Simon G.
Martínez Serra, Pedro
author Perea Atienza, Elena
author_facet Perea Atienza, Elena
Sprecher, Simon G.
Martínez Serra, Pedro
author_role author
author2 Sprecher, Simon G.
Martínez Serra, Pedro
author2_role author
author
dc.subject.none.fl_str_mv Neurobiologia
Embriologia
Transcripció genètica
Invertebrats
Neurobiology
Embryology
Genetic transcription
Invertebrates
topic Neurobiologia
Embriologia
Transcripció genètica
Invertebrats
Neurobiology
Embryology
Genetic transcription
Invertebrates
description Background: The basic helix-loop-helix (bHLH) family of transcription factors is one of the largest superfamilies of regulatory transcription factors and is widely used in eukaryotic organisms. They play an essential role in a range of metabolic, physiological, and developmental processes, including the development of the nervous system (NS). These transcription factors have been studied in many metazoans, especially in vertebrates but also in early branching metazoan clades such as the cnidarians and sponges. However, currently very little is known about their expression in the most basally branching bilaterian group, the xenacoelomorphs. Recently, our laboratory has characterized the full complement of bHLH in the genome of two members of the Xenacoelomorpha, the xenoturbellid Xenoturbella bocki and the acoel Symsagittifera roscoffensis. Understanding the patterns of bHLH gene expression in members of this phylum (in space and time) provides critical new insights into the conserved roles of the bHLH and their putative specificities in this group. Our focus is on deciphering the specific roles that these genes have in the process of neurogenesis. Results: Here, we analyze the developmental expression of the whole complement of bHLH genes identified in the acoel S. roscoffensis. Based on their expression patterns, several members of bHLH class A appear to have specific conserved roles in neurogenesis, while other class A genes (as well as members of other classes) have likely taken on more generalized functions. All gene expression patterns are described in embryos and early juveniles. Conclusion: Our results suggest that the main roles of the bHLH genes of S. roscoffensis are evolutionarily conserved, with a specific subset dedicated to patterning the nervous system: SrAscA, SrAscB, SrHes/Hey, SrNscl, SrSrebp, SrE12/ E47 and SrOlig.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/122079
url https://hdl.handle.net/2445/122079
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1186/s13227-018-0097-y
Evodevo, 2018, vol. 9, num. 8
https://doi.org/10.1186/s13227-018-0097-y
dc.rights.none.fl_str_mv cc-by (c) Perea-Atienza, E. et al., 2018
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Perea-Atienza, E. et al., 2018
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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