Oxidative stress, lipid peroxidation and ferroptosis are major pathophysiological signatures in the placental tissue of women with late-onset preeclampsia
Preeclampsia, a serious and potentially life-threatening medical complication occurring during pregnancy, is characterized by hypertension and often accompanied by proteinuria and multiorgan dysfunction. It is classified into two subtypes based on the timing of diagnosis: early-onset (EO-PE) and lat...
| Autores: | , , , , , , , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Universidad de Alcalá (UAH) |
| Repositorio: | e_Buah Biblioteca Digital Universidad de Alcalá |
| Idioma: | inglés |
| OAI Identifier: | oai:ebuah.uah.es:10017/67811 |
| Acceso en línea: | http://hdl.handle.net/10017/67811 https://dx.doi.org/10.3390/antiox13050591 |
| Access Level: | acceso abierto |
| Palabra clave: | Oxidative stress Lipid peroxidation Ferroptosis Placental Preeclampsia Medicina Medicine |
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Oxidative stress, lipid peroxidation and ferroptosis are major pathophysiological signatures in the placental tissue of women with late-onset preeclampsiaOrtega, Miguel A.García Puente, Luis M.Fraile Martínez, Óscar|||0000-0002-4494-6397Pekarek, TatianaGarcía Montero, Cielo|||0000-0001-6016-7855Bujan, JuliánPekarek, Leonel|||0000-0002-5966-9003Barrena Blázquez, Silvestra|||0000-0003-2715-1979Gragera Martínez, Raquel de los Reyes|||0000-0001-5694-8579Rodríguez Rojo, Inmaculada C.Rodríguez Benítez, PatrocinioLópez González, Laura|||0009-0005-1023-7639Díaz Pedrero, Raúl|||0000-0001-9318-4045Álvarez de Mon Soto, Melchor|||0000-0003-1309-7510García Honduvilla, Natalio Antonio|||0000-0003-0904-6198León Luis, Juan A. deBravo Arribas, CoralSaez, Miguel A.Oxidative stressLipid peroxidationFerroptosisPlacentalPreeclampsiaMedicinaMedicinePreeclampsia, a serious and potentially life-threatening medical complication occurring during pregnancy, is characterized by hypertension and often accompanied by proteinuria and multiorgan dysfunction. It is classified into two subtypes based on the timing of diagnosis: early-onset (EO-PE) and late-onset preeclampsia (LO-PE). Despite being less severe and exhibiting distinct pathophysiological characteristics, LO-PE is more prevalent than EO-PE, although both conditions have a significant impact on placental health. Previous research indicates that different pathophysiological events within the placenta may contribute to the development of preeclampsia across multiple pathways. In our experimental study, we investigated markers of oxidative stress, ferroptosis, and lipid peroxidation pathways in placental tissue samples obtained from women with LO-PE (n = 68) compared to healthy control pregnant women (HC, n = 43). Through a comprehensive analysis, we observed an upregulation of specific molecules associated with these pathways, including NADPH oxidase 1 (NOX-1), NADPH oxidase 2 (NOX-2), transferrin receptor protein 1 (TFRC), arachidonate 5-lipoxygenase (ALOX-5), acyl-CoA synthetase long-chain family member 4 (ACSL-4), glutathione peroxidase 4 (GPX4) and malondialdehyde (MDA) in women with LO-PE. Furthermore, increased ferric tissue deposition (Fe3+) was observed in placenta samples stained with Perls’ Prussian blue. The assessment involved gene and protein expression analyses conducted through RT-qPCR experiments and immunohistochemistry assays. Our findings underscore the heightened activation of inflammatory pathways in LO-PE compared to HC, highlighting the pathological mechanisms underlying this pregnancy disorder.Comunidad de MadridInstituto de Salud Carlos III20242024-05-01journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10017/67811https://dx.doi.org/10.3390/antiox1305059138790696reponame:e_Buah Biblioteca Digital Universidad de Alcaláinstname:Universidad de Alcalá (UAH)InglésengCM Not available S2022%2FBMD-7406 RADIOPROTECT-CMISCIII AES 2021. Proyectos de investigación en salud FIS-PI21%2F01244 INSUFICIENCIA PLACENTARIA SECUNDARIA A HIPERTENSIÓN VENOSA GESTACIONAL: INFLUENCIA DEL ESTADO INFLAMATORIOopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ebuah.uah.es:10017/678112026-06-18T11:13:07Z |
| dc.title.none.fl_str_mv |
Oxidative stress, lipid peroxidation and ferroptosis are major pathophysiological signatures in the placental tissue of women with late-onset preeclampsia |
| title |
Oxidative stress, lipid peroxidation and ferroptosis are major pathophysiological signatures in the placental tissue of women with late-onset preeclampsia |
| spellingShingle |
Oxidative stress, lipid peroxidation and ferroptosis are major pathophysiological signatures in the placental tissue of women with late-onset preeclampsia Ortega, Miguel A. Oxidative stress Lipid peroxidation Ferroptosis Placental Preeclampsia Medicina Medicine |
| title_short |
Oxidative stress, lipid peroxidation and ferroptosis are major pathophysiological signatures in the placental tissue of women with late-onset preeclampsia |
| title_full |
Oxidative stress, lipid peroxidation and ferroptosis are major pathophysiological signatures in the placental tissue of women with late-onset preeclampsia |
| title_fullStr |
Oxidative stress, lipid peroxidation and ferroptosis are major pathophysiological signatures in the placental tissue of women with late-onset preeclampsia |
| title_full_unstemmed |
Oxidative stress, lipid peroxidation and ferroptosis are major pathophysiological signatures in the placental tissue of women with late-onset preeclampsia |
| title_sort |
Oxidative stress, lipid peroxidation and ferroptosis are major pathophysiological signatures in the placental tissue of women with late-onset preeclampsia |
| dc.creator.none.fl_str_mv |
Ortega, Miguel A. García Puente, Luis M. Fraile Martínez, Óscar|||0000-0002-4494-6397 Pekarek, Tatiana García Montero, Cielo|||0000-0001-6016-7855 Bujan, Julián Pekarek, Leonel|||0000-0002-5966-9003 Barrena Blázquez, Silvestra|||0000-0003-2715-1979 Gragera Martínez, Raquel de los Reyes|||0000-0001-5694-8579 Rodríguez Rojo, Inmaculada C. Rodríguez Benítez, Patrocinio López González, Laura|||0009-0005-1023-7639 Díaz Pedrero, Raúl|||0000-0001-9318-4045 Álvarez de Mon Soto, Melchor|||0000-0003-1309-7510 García Honduvilla, Natalio Antonio|||0000-0003-0904-6198 León Luis, Juan A. de Bravo Arribas, Coral Saez, Miguel A. |
| author |
Ortega, Miguel A. |
| author_facet |
Ortega, Miguel A. García Puente, Luis M. Fraile Martínez, Óscar|||0000-0002-4494-6397 Pekarek, Tatiana García Montero, Cielo|||0000-0001-6016-7855 Bujan, Julián Pekarek, Leonel|||0000-0002-5966-9003 Barrena Blázquez, Silvestra|||0000-0003-2715-1979 Gragera Martínez, Raquel de los Reyes|||0000-0001-5694-8579 Rodríguez Rojo, Inmaculada C. Rodríguez Benítez, Patrocinio López González, Laura|||0009-0005-1023-7639 Díaz Pedrero, Raúl|||0000-0001-9318-4045 Álvarez de Mon Soto, Melchor|||0000-0003-1309-7510 García Honduvilla, Natalio Antonio|||0000-0003-0904-6198 León Luis, Juan A. de Bravo Arribas, Coral Saez, Miguel A. |
| author_role |
author |
| author2 |
García Puente, Luis M. Fraile Martínez, Óscar|||0000-0002-4494-6397 Pekarek, Tatiana García Montero, Cielo|||0000-0001-6016-7855 Bujan, Julián Pekarek, Leonel|||0000-0002-5966-9003 Barrena Blázquez, Silvestra|||0000-0003-2715-1979 Gragera Martínez, Raquel de los Reyes|||0000-0001-5694-8579 Rodríguez Rojo, Inmaculada C. Rodríguez Benítez, Patrocinio López González, Laura|||0009-0005-1023-7639 Díaz Pedrero, Raúl|||0000-0001-9318-4045 Álvarez de Mon Soto, Melchor|||0000-0003-1309-7510 García Honduvilla, Natalio Antonio|||0000-0003-0904-6198 León Luis, Juan A. de Bravo Arribas, Coral Saez, Miguel A. |
| author2_role |
author author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Oxidative stress Lipid peroxidation Ferroptosis Placental Preeclampsia Medicina Medicine |
| topic |
Oxidative stress Lipid peroxidation Ferroptosis Placental Preeclampsia Medicina Medicine |
| description |
Preeclampsia, a serious and potentially life-threatening medical complication occurring during pregnancy, is characterized by hypertension and often accompanied by proteinuria and multiorgan dysfunction. It is classified into two subtypes based on the timing of diagnosis: early-onset (EO-PE) and late-onset preeclampsia (LO-PE). Despite being less severe and exhibiting distinct pathophysiological characteristics, LO-PE is more prevalent than EO-PE, although both conditions have a significant impact on placental health. Previous research indicates that different pathophysiological events within the placenta may contribute to the development of preeclampsia across multiple pathways. In our experimental study, we investigated markers of oxidative stress, ferroptosis, and lipid peroxidation pathways in placental tissue samples obtained from women with LO-PE (n = 68) compared to healthy control pregnant women (HC, n = 43). Through a comprehensive analysis, we observed an upregulation of specific molecules associated with these pathways, including NADPH oxidase 1 (NOX-1), NADPH oxidase 2 (NOX-2), transferrin receptor protein 1 (TFRC), arachidonate 5-lipoxygenase (ALOX-5), acyl-CoA synthetase long-chain family member 4 (ACSL-4), glutathione peroxidase 4 (GPX4) and malondialdehyde (MDA) in women with LO-PE. Furthermore, increased ferric tissue deposition (Fe3+) was observed in placenta samples stained with Perls’ Prussian blue. The assessment involved gene and protein expression analyses conducted through RT-qPCR experiments and immunohistochemistry assays. Our findings underscore the heightened activation of inflammatory pathways in LO-PE compared to HC, highlighting the pathological mechanisms underlying this pregnancy disorder. |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2024 2024-05-01 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 NA http://purl.org/coar/version/c_be7fb7dd8ff6fe43 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10017/67811 https://dx.doi.org/10.3390/antiox13050591 38790696 |
| url |
http://hdl.handle.net/10017/67811 https://dx.doi.org/10.3390/antiox13050591 |
| identifier_str_mv |
38790696 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.relation.none.fl_str_mv |
CM Not available S2022%2FBMD-7406 RADIOPROTECT-CM ISCIII AES 2021. Proyectos de investigación en salud FIS-PI21%2F01244 INSUFICIENCIA PLACENTARIA SECUNDARIA A HIPERTENSIÓN VENOSA GESTACIONAL: INFLUENCIA DEL ESTADO INFLAMATORIO |
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open access http://purl.org/coar/access_right/c_abf2 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
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openAccess |
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application/pdf |
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reponame:e_Buah Biblioteca Digital Universidad de Alcalá instname:Universidad de Alcalá (UAH) |
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e_Buah Biblioteca Digital Universidad de Alcalá |
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e_Buah Biblioteca Digital Universidad de Alcalá |
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