Bioprinting Decellularized Breast Tissue for the Development of Three-Dimensional Breast Cancer Models

The tumor extracellular matrix (ECM) plays a vital role in tumor progression and drug resistance. Previous studies have shown that breast tissue-derived matrices could be an important biomaterial to recreate the complexity of the tumor ECM. We have developed a method for decellularizing and delipida...

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Autores: Blanco Fernandez, Barbara, Rey Vinolas, Sergi, Bagci Gulsun, Rubi Sans, Gerard, Otero Díaz, Jorge, Navajas, Daniel, Pérez Amodio, Soledad, Engel López, Elisabeth
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/216396
Acceso en línea:https://hdl.handle.net/2445/216396
Access Level:acceso abierto
Palabra clave:Càncer de mama
Enginyeria de teixits
Matriu extracel·lular
Breast cancer
Tissue engineering
Extracellular matrix
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spelling Bioprinting Decellularized Breast Tissue for the Development of Three-Dimensional Breast Cancer ModelsBlanco Fernandez, BarbaraRey Vinolas, SergiBagci GulsunRubi Sans, GerardOtero Díaz, JorgeNavajas, DanielPérez Amodio, SoledadEngel López, ElisabethCàncer de mamaEnginyeria de teixitsMatriu extracel·lularBreast cancerTissue engineeringExtracellular matrixThe tumor extracellular matrix (ECM) plays a vital role in tumor progression and drug resistance. Previous studies have shown that breast tissue-derived matrices could be an important biomaterial to recreate the complexity of the tumor ECM. We have developed a method for decellularizing and delipidating a porcine breast tissue (TDM) compatible with hydrogel formation. The addition of gelatin methacrylamide and alginate allows this TDM to be bioprinted by itself with good printability, shape fidelity, and cytocompatibility. Furthermore, this bioink has been tuned to more closely recreate the breast tumor by incorporating collagen type I (Col1). Breast cancer cells (BCCs) proliferate in both TDM bioinks forming cell clusters and spheroids. The addition of Col1 improves the printability of the bioink as well as increases BCC proliferation and reduces doxorubicin sensitivity due to a downregulation of HSP90. TDM bioinks also allow a precise three-dimensional printing of scaffolds containing BCCs and stromal cells and could be used to fabricate artificial tumors. Taken together, we have proven that these novel bioinks are good candidates for biofabricating breast cancer models.American Chemical Society2024202420222024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion16 p.application/pdfhttps://hdl.handle.net/2445/216396Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1021/acsami.2c00920ACS Applied Materials & Interfaces, 2022, vol. 14, num.26, p. 29467-29482https://doi.org/10.1021/acsami.2c00920cc-by (c) Blanco Fernandez et al., 2022http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/2163962026-05-29T05:05:01Z
dc.title.none.fl_str_mv Bioprinting Decellularized Breast Tissue for the Development of Three-Dimensional Breast Cancer Models
title Bioprinting Decellularized Breast Tissue for the Development of Three-Dimensional Breast Cancer Models
spellingShingle Bioprinting Decellularized Breast Tissue for the Development of Three-Dimensional Breast Cancer Models
Blanco Fernandez, Barbara
Càncer de mama
Enginyeria de teixits
Matriu extracel·lular
Breast cancer
Tissue engineering
Extracellular matrix
title_short Bioprinting Decellularized Breast Tissue for the Development of Three-Dimensional Breast Cancer Models
title_full Bioprinting Decellularized Breast Tissue for the Development of Three-Dimensional Breast Cancer Models
title_fullStr Bioprinting Decellularized Breast Tissue for the Development of Three-Dimensional Breast Cancer Models
title_full_unstemmed Bioprinting Decellularized Breast Tissue for the Development of Three-Dimensional Breast Cancer Models
title_sort Bioprinting Decellularized Breast Tissue for the Development of Three-Dimensional Breast Cancer Models
dc.creator.none.fl_str_mv Blanco Fernandez, Barbara
Rey Vinolas, Sergi
Bagci Gulsun
Rubi Sans, Gerard
Otero Díaz, Jorge
Navajas, Daniel
Pérez Amodio, Soledad
Engel López, Elisabeth
author Blanco Fernandez, Barbara
author_facet Blanco Fernandez, Barbara
Rey Vinolas, Sergi
Bagci Gulsun
Rubi Sans, Gerard
Otero Díaz, Jorge
Navajas, Daniel
Pérez Amodio, Soledad
Engel López, Elisabeth
author_role author
author2 Rey Vinolas, Sergi
Bagci Gulsun
Rubi Sans, Gerard
Otero Díaz, Jorge
Navajas, Daniel
Pérez Amodio, Soledad
Engel López, Elisabeth
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Càncer de mama
Enginyeria de teixits
Matriu extracel·lular
Breast cancer
Tissue engineering
Extracellular matrix
topic Càncer de mama
Enginyeria de teixits
Matriu extracel·lular
Breast cancer
Tissue engineering
Extracellular matrix
description The tumor extracellular matrix (ECM) plays a vital role in tumor progression and drug resistance. Previous studies have shown that breast tissue-derived matrices could be an important biomaterial to recreate the complexity of the tumor ECM. We have developed a method for decellularizing and delipidating a porcine breast tissue (TDM) compatible with hydrogel formation. The addition of gelatin methacrylamide and alginate allows this TDM to be bioprinted by itself with good printability, shape fidelity, and cytocompatibility. Furthermore, this bioink has been tuned to more closely recreate the breast tumor by incorporating collagen type I (Col1). Breast cancer cells (BCCs) proliferate in both TDM bioinks forming cell clusters and spheroids. The addition of Col1 improves the printability of the bioink as well as increases BCC proliferation and reduces doxorubicin sensitivity due to a downregulation of HSP90. TDM bioinks also allow a precise three-dimensional printing of scaffolds containing BCCs and stromal cells and could be used to fabricate artificial tumors. Taken together, we have proven that these novel bioinks are good candidates for biofabricating breast cancer models.
publishDate 2022
dc.date.none.fl_str_mv 2022
2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/216396
url https://hdl.handle.net/2445/216396
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1021/acsami.2c00920
ACS Applied Materials & Interfaces, 2022, vol. 14, num.26, p. 29467-29482
https://doi.org/10.1021/acsami.2c00920
dc.rights.none.fl_str_mv cc-by (c) Blanco Fernandez et al., 2022
http://creativecommons.org/licenses/by/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Blanco Fernandez et al., 2022
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 16 p.
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869414973754572800
score 15,811543

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