Unravelling the capacity of hydroxytyrosol and its lipophenolic derivates to modulate the H2O2-induced isoprostanoid profile of THP-1 monocytes by UHPLC-QqQ-MS/MS lipidomic workflow

Presently, the attention given to natural substances to counteract damage produced by oxidative stress (OS) has risen sharply. In this scenario, hydroxytyrosol (HT) derivatives, formed as a result of HT conjugation with fatty acids (FAs) (lipophenols), have been recently described in foodstuffs such...

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Autores: Alemán Jiménez, Carolina, Domínguez-Perles, Raúl, Fanti, Federico, Gallego-Gomez, Juana I., Simonelli Muñoz, Agustín, Moine, Espérance, Durand, Thierry, Crauste, Céline, Gil-Izquierdo, Ángel, Medina Escudero, Sonia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/254353
Acceso en línea:http://hdl.handle.net/10261/254353
Access Level:acceso abierto
Palabra clave:Hydroxytyrosol
Lipophenols
In vitro models
Isoprostanoids
Oxidative stress
Inflammation
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spelling Unravelling the capacity of hydroxytyrosol and its lipophenolic derivates to modulate the H2O2-induced isoprostanoid profile of THP-1 monocytes by UHPLC-QqQ-MS/MS lipidomic workflowAlemán Jiménez, CarolinaDomínguez-Perles, RaúlFanti, FedericoGallego-Gomez, Juana I.Simonelli Muñoz, AgustínMoine, EspéranceDurand, ThierryCrauste, CélineGil-Izquierdo, ÁngelMedina Escudero, SoniaHydroxytyrosolLipophenolsIn vitro modelsIsoprostanoidsOxidative stressInflammationPresently, the attention given to natural substances to counteract damage produced by oxidative stress (OS) has risen sharply. In this scenario, hydroxytyrosol (HT) derivatives, formed as a result of HT conjugation with fatty acids (FAs) (lipophenols), have been recently described in foodstuffs such as extra virgin olive oil, as being powerful bioactive compounds with a higher activity than the unesterified phenolic compound. The present work describes the capacity of HT lipophenols to act on the course of OS and secondary inflammatory processes, based on their capacity to modulate the isoprostanoid profile induced by HO in THP-1 monocytic cells. A UHPLC-QqQ-ESI-MS/MS-based lipidomics workflow was applied over a range of 37 human oxylipins. The main outcomes retrieved suggest both HT and HT-lipophenols as regulators of the cellular redox balance, acting as pro-oxidants in vitro, which is highly dependent on the experimental conditions. Our outcomes suggest the anti-inflammatory potential of both HT and HT-lipophenols, where the type of the FAs on the HT core appears to be critical for defining the bioactivity of lipophenols, highlighting that a lipidomic approach, with the simultaneous analysis of multiple oxylipins, is critical for the understanding of the bioactivity of lipophenols on isoprostanoid generation and hence, on pathophysiological processes.This work was partially funded by the “Fundación Séneca de la Región de Murcia” Grupo de Excelencia 19900/GERM/15 and the Spanish project AGL2017-83386-R-AEI/FEDER, UE from the Spanish Ministry of Science, Innovation, and Universities. SM was supported by a Postdoctoral Contract (Saavedra-Fajardo 21078/SF/19) from the Agency for Science and Technology of the Región de Murcia – “Fundación Séneca” (Spain)Elsevier BVFundación SénecaMinisterio de Economía y Competitividad (España)Agencia Estatal de Investigación (España)European CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2021202120212021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/254353reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-83386-Rhttp://dx.doi.org/10.1016/j.microc.2021.106703Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2543532026-05-22T06:33:51Z
dc.title.none.fl_str_mv Unravelling the capacity of hydroxytyrosol and its lipophenolic derivates to modulate the H2O2-induced isoprostanoid profile of THP-1 monocytes by UHPLC-QqQ-MS/MS lipidomic workflow
title Unravelling the capacity of hydroxytyrosol and its lipophenolic derivates to modulate the H2O2-induced isoprostanoid profile of THP-1 monocytes by UHPLC-QqQ-MS/MS lipidomic workflow
spellingShingle Unravelling the capacity of hydroxytyrosol and its lipophenolic derivates to modulate the H2O2-induced isoprostanoid profile of THP-1 monocytes by UHPLC-QqQ-MS/MS lipidomic workflow
Alemán Jiménez, Carolina
Hydroxytyrosol
Lipophenols
In vitro models
Isoprostanoids
Oxidative stress
Inflammation
title_short Unravelling the capacity of hydroxytyrosol and its lipophenolic derivates to modulate the H2O2-induced isoprostanoid profile of THP-1 monocytes by UHPLC-QqQ-MS/MS lipidomic workflow
title_full Unravelling the capacity of hydroxytyrosol and its lipophenolic derivates to modulate the H2O2-induced isoprostanoid profile of THP-1 monocytes by UHPLC-QqQ-MS/MS lipidomic workflow
title_fullStr Unravelling the capacity of hydroxytyrosol and its lipophenolic derivates to modulate the H2O2-induced isoprostanoid profile of THP-1 monocytes by UHPLC-QqQ-MS/MS lipidomic workflow
title_full_unstemmed Unravelling the capacity of hydroxytyrosol and its lipophenolic derivates to modulate the H2O2-induced isoprostanoid profile of THP-1 monocytes by UHPLC-QqQ-MS/MS lipidomic workflow
title_sort Unravelling the capacity of hydroxytyrosol and its lipophenolic derivates to modulate the H2O2-induced isoprostanoid profile of THP-1 monocytes by UHPLC-QqQ-MS/MS lipidomic workflow
dc.creator.none.fl_str_mv Alemán Jiménez, Carolina
Domínguez-Perles, Raúl
Fanti, Federico
Gallego-Gomez, Juana I.
Simonelli Muñoz, Agustín
Moine, Espérance
Durand, Thierry
Crauste, Céline
Gil-Izquierdo, Ángel
Medina Escudero, Sonia
author Alemán Jiménez, Carolina
author_facet Alemán Jiménez, Carolina
Domínguez-Perles, Raúl
Fanti, Federico
Gallego-Gomez, Juana I.
Simonelli Muñoz, Agustín
Moine, Espérance
Durand, Thierry
Crauste, Céline
Gil-Izquierdo, Ángel
Medina Escudero, Sonia
author_role author
author2 Domínguez-Perles, Raúl
Fanti, Federico
Gallego-Gomez, Juana I.
Simonelli Muñoz, Agustín
Moine, Espérance
Durand, Thierry
Crauste, Céline
Gil-Izquierdo, Ángel
Medina Escudero, Sonia
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Fundación Séneca
Ministerio de Economía y Competitividad (España)
Agencia Estatal de Investigación (España)
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Hydroxytyrosol
Lipophenols
In vitro models
Isoprostanoids
Oxidative stress
Inflammation
topic Hydroxytyrosol
Lipophenols
In vitro models
Isoprostanoids
Oxidative stress
Inflammation
description Presently, the attention given to natural substances to counteract damage produced by oxidative stress (OS) has risen sharply. In this scenario, hydroxytyrosol (HT) derivatives, formed as a result of HT conjugation with fatty acids (FAs) (lipophenols), have been recently described in foodstuffs such as extra virgin olive oil, as being powerful bioactive compounds with a higher activity than the unesterified phenolic compound. The present work describes the capacity of HT lipophenols to act on the course of OS and secondary inflammatory processes, based on their capacity to modulate the isoprostanoid profile induced by HO in THP-1 monocytic cells. A UHPLC-QqQ-ESI-MS/MS-based lipidomics workflow was applied over a range of 37 human oxylipins. The main outcomes retrieved suggest both HT and HT-lipophenols as regulators of the cellular redox balance, acting as pro-oxidants in vitro, which is highly dependent on the experimental conditions. Our outcomes suggest the anti-inflammatory potential of both HT and HT-lipophenols, where the type of the FAs on the HT core appears to be critical for defining the bioactivity of lipophenols, highlighting that a lipidomic approach, with the simultaneous analysis of multiple oxylipins, is critical for the understanding of the bioactivity of lipophenols on isoprostanoid generation and hence, on pathophysiological processes.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/254353
url http://hdl.handle.net/10261/254353
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-83386-R
http://dx.doi.org/10.1016/j.microc.2021.106703
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier BV
publisher.none.fl_str_mv Elsevier BV
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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