Cooling rate modifies the location of aquaporin 3 in spermatozoa of sheep and goat

The freeze-thawing process induces osmotic changes that may affect the membrane domain location of aquaporins’ (AQP) in spermatozoa. Recent studies suggest that changes in AQP3 localization allows better sperm osmo-adaptation, improving the cryoresistance. Ultra-rapid freezing is an alternative cryo...

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Autores: Pequeño, Belén, Millán de la Blanca, María Gema, Castaño, Cristina, Toledano Díaz, Adolfo, Esteso, Milagros C., Alba, Esther, Arrebola, Francisco A., Ungerfeld, Rodolfo, Martínez Madrid, Carmen Belén, Álvarez Rodríguez, Manuel, Rodríguez Martínez, Heriberto, Santiago Moreno, Julián
Formato: artículo
Fecha de publicación:2024
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/103756
Acesso em linha:https://hdl.handle.net/20.500.14352/103756
Access Level:acceso abierto
Palavra-chave:636.09
Aquaporins
Domestic ruminants
Sperm
Cryoresistance
Veterinaria
3109 Ciencias Veterinarias
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oai_identifier_str oai:docta.ucm.es:20.500.14352/103756
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network_name_str España
repository_id_str
spelling Cooling rate modifies the location of aquaporin 3 in spermatozoa of sheep and goatPequeño, BelénMillán de la Blanca, María GemaCastaño, CristinaToledano Díaz, AdolfoEsteso, Milagros C.Alba, EstherArrebola, Francisco A.Ungerfeld, RodolfoMartínez Madrid, Carmen BelénÁlvarez Rodríguez, ManuelRodríguez Martínez, HeribertoSantiago Moreno, Julián636.09AquaporinsDomestic ruminantsSpermCryoresistanceVeterinaria3109 Ciencias VeterinariasThe freeze-thawing process induces osmotic changes that may affect the membrane domain location of aquaporins’ (AQP) in spermatozoa. Recent studies suggest that changes in AQP3 localization allows better sperm osmo-adaptation, improving the cryoresistance. Ultra-rapid freezing is an alternative cryopreservation technique that requires less equipment than conventional freezing, and it is faster, simpler and can be used in the field. This study aimed to determine the influence of freezing-thawing rates (slow (control) vs. ultra-rapid) on AQP3 expression and location in the spermatozoa from small ruminants (sheep and goats) and its relationship with sperm cryo-damage. Spermatozoa were collected from 10 Merino rams and 10 Murciano-Granadina bucks. The presence and distribution of AQP3 were assessed by Western blotting and immunocytochemistry (ICC), employing a commercial rabbit polyclonal antibody. Sperm motility was CASA system-analyzed, and membrane and acrosome integrity assessed by fluorescence (PI/PNA-FITC). Western blotting did not detect a significant effect of freezing-thawing rate on the amount of AQP3 while ICC found freezing-thawing rate affecting AQP3 location (P < 0.05). In both species, the percentages of spermatozoa showing AQP3 in the post-acrosome region, mid-piece, and principal piece of the tail were greater in samples cryopreserved by slow freezing-thawing (control) than ultra-rapid freezing-thawing rates (P < 0.05). Spermatozoa cryopreserved using ultra-rapid freezing-thawing showed decrease motility, plasma membrane, and acrosome integrity (P < 0.05), which might be related, at least in part, to a lower expression of AQP3. In conclusion, the cooling rate modifies the location of AQP3 in spermatozoa of sheep and goat, which might be associated with sperm cryosurvival.ElsevierUniversidad Complutense de Madrid20242024-04-1920242024-04-19journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/103756reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)InglésengMCIU PID2020 113288RB-I00Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 10.13039 501100011033open accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1037562026-06-02T12:44:21Z
dc.title.none.fl_str_mv Cooling rate modifies the location of aquaporin 3 in spermatozoa of sheep and goat
title Cooling rate modifies the location of aquaporin 3 in spermatozoa of sheep and goat
spellingShingle Cooling rate modifies the location of aquaporin 3 in spermatozoa of sheep and goat
Pequeño, Belén
636.09
Aquaporins
Domestic ruminants
Sperm
Cryoresistance
Veterinaria
3109 Ciencias Veterinarias
title_short Cooling rate modifies the location of aquaporin 3 in spermatozoa of sheep and goat
title_full Cooling rate modifies the location of aquaporin 3 in spermatozoa of sheep and goat
title_fullStr Cooling rate modifies the location of aquaporin 3 in spermatozoa of sheep and goat
title_full_unstemmed Cooling rate modifies the location of aquaporin 3 in spermatozoa of sheep and goat
title_sort Cooling rate modifies the location of aquaporin 3 in spermatozoa of sheep and goat
dc.creator.none.fl_str_mv Pequeño, Belén
Millán de la Blanca, María Gema
Castaño, Cristina
Toledano Díaz, Adolfo
Esteso, Milagros C.
Alba, Esther
Arrebola, Francisco A.
Ungerfeld, Rodolfo
Martínez Madrid, Carmen Belén
Álvarez Rodríguez, Manuel
Rodríguez Martínez, Heriberto
Santiago Moreno, Julián
author Pequeño, Belén
author_facet Pequeño, Belén
Millán de la Blanca, María Gema
Castaño, Cristina
Toledano Díaz, Adolfo
Esteso, Milagros C.
Alba, Esther
Arrebola, Francisco A.
Ungerfeld, Rodolfo
Martínez Madrid, Carmen Belén
Álvarez Rodríguez, Manuel
Rodríguez Martínez, Heriberto
Santiago Moreno, Julián
author_role author
author2 Millán de la Blanca, María Gema
Castaño, Cristina
Toledano Díaz, Adolfo
Esteso, Milagros C.
Alba, Esther
Arrebola, Francisco A.
Ungerfeld, Rodolfo
Martínez Madrid, Carmen Belén
Álvarez Rodríguez, Manuel
Rodríguez Martínez, Heriberto
Santiago Moreno, Julián
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 636.09
Aquaporins
Domestic ruminants
Sperm
Cryoresistance
Veterinaria
3109 Ciencias Veterinarias
topic 636.09
Aquaporins
Domestic ruminants
Sperm
Cryoresistance
Veterinaria
3109 Ciencias Veterinarias
description The freeze-thawing process induces osmotic changes that may affect the membrane domain location of aquaporins’ (AQP) in spermatozoa. Recent studies suggest that changes in AQP3 localization allows better sperm osmo-adaptation, improving the cryoresistance. Ultra-rapid freezing is an alternative cryopreservation technique that requires less equipment than conventional freezing, and it is faster, simpler and can be used in the field. This study aimed to determine the influence of freezing-thawing rates (slow (control) vs. ultra-rapid) on AQP3 expression and location in the spermatozoa from small ruminants (sheep and goats) and its relationship with sperm cryo-damage. Spermatozoa were collected from 10 Merino rams and 10 Murciano-Granadina bucks. The presence and distribution of AQP3 were assessed by Western blotting and immunocytochemistry (ICC), employing a commercial rabbit polyclonal antibody. Sperm motility was CASA system-analyzed, and membrane and acrosome integrity assessed by fluorescence (PI/PNA-FITC). Western blotting did not detect a significant effect of freezing-thawing rate on the amount of AQP3 while ICC found freezing-thawing rate affecting AQP3 location (P < 0.05). In both species, the percentages of spermatozoa showing AQP3 in the post-acrosome region, mid-piece, and principal piece of the tail were greater in samples cryopreserved by slow freezing-thawing (control) than ultra-rapid freezing-thawing rates (P < 0.05). Spermatozoa cryopreserved using ultra-rapid freezing-thawing showed decrease motility, plasma membrane, and acrosome integrity (P < 0.05), which might be related, at least in part, to a lower expression of AQP3. In conclusion, the cooling rate modifies the location of AQP3 in spermatozoa of sheep and goat, which might be associated with sperm cryosurvival.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-04-19
2024
2024-04-19
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/103756
url https://hdl.handle.net/20.500.14352/103756
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv MCIU PID2020 113288RB-I00
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 10.13039 501100011033
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300719

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