Caracterización de la actividad proteasa digestiva de atún rojo Thunnus thynnus (Linnaeus, 1758)
The digestive proteases of bluefin tuna Thunnus thynnus (Linnaeus, 1758) were studied. Protease activity in stomach extracts showed two optima, at pH 2.0 and 3.5. Optimum activity for intestinal proteases was found at pH 10.0 and 12.0. Protease activity in stomach extracts was found to be stable at...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2002 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/313287 |
| Acceso en línea: | http://hdl.handle.net/10261/313287 |
| Access Level: | acceso abierto |
| Palabra clave: | Acuicultura Estómago intestino Inhibidores de proteasas electroforesis proteasa digestiva zimograma |
| Sumario: | The digestive proteases of bluefin tuna Thunnus thynnus (Linnaeus, 1758) were studied. Protease activity in stomach extracts showed two optima, at pH 2.0 and 3.5. Optimum activity for intestinal proteases was found at pH 10.0 and 12.0. Protease activity in stomach extracts was found to be stable at several pH, except for pH 12.0. Alkaline protease activity of intestine extracts was highly sensitive to acidic pH. Optimal temperatures found were 50 ºC for acid proteases and 60 ºC for alkaline proteases. The use of specific protease inhibitors indicated that the main presence of acid proteases was in stomach extracts, and serine proteases in intestine extracts. Active fractions in extracts were identified with SDS-PAGE. |
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