Genetic diversity of <em>Phlebotomus perniciosus</em>populations between insular and mainland regionsin the leishmaniasis-endemic westernMediterranean area

Phlebotomus perniciosus</em> is the primary vector of <em>Leishmania infantum</em> in Spain, occurring in both continental and insular regions. This study investigates the genetic structure of <em>P. perniciosus</em> populations from Majorca (island) and Barcelona (main...

ver descrição completa

Detalhes bibliográficos
Autores: Chavez-Fisa, Sarah, Roca Geronès, Xavier, Fisa Saladrigas, Roser, Riera Lizandra, Ma. Cristina, Alcover Amengual, Maria Magdalena
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2026
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositório:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/226396
Acesso em linha:https://hdl.handle.net/2445/226396
Access Level:Acceso aberto
Palavra-chave:Illes Balears
Leishmania infantum
Leishmaniosi
Balearic Islands
Leishmaniasis
id ES_9fcf4dc6b673d41ecef97a5b7f5f5c54
oai_identifier_str oai:recercat.cat:2445/226396
network_acronym_str ES
network_name_str España
repository_id_str
spelling Genetic diversity of <em>Phlebotomus perniciosus</em>populations between insular and mainland regionsin the leishmaniasis-endemic westernMediterranean areaChavez-Fisa, SarahRoca Geronès, XavierFisa Saladrigas, RoserRiera Lizandra, Ma. CristinaAlcover Amengual, Maria MagdalenaIlles BalearsLeishmania infantumLeishmaniosiBalearic IslandsLeishmania infantumLeishmaniasisPhlebotomus perniciosus</em> is the primary vector of <em>Leishmania infantum</em> in Spain, occurring in both continental and insular regions. This study investigates the genetic structure of <em>P. perniciosus</em> populations from Majorca (island) and Barcelona (mainland), two geographically close but ecologically distinct regions in the western Mediterranean.</p><p><strong>Methods: </strong>Mitochondrial cytochrome c oxidase subunit I (COI) gene sequences were analyzed from 167 <em>P. perniciosus</em> specimens, including 100 morphologically identified field-collected specimens from Majorca and Barcelona, supplemented with reference data from the South and West Iberian Peninsula and North Africa. Population differentiation was assessed using genetic diversity indices, Bayesian phylogenetic inference, analysis of molecular variance (AMOVA), pairwise Fst values and Nm estimates, haplotype networks, and a Mantel test.</p><p><strong>Results:</strong> Phylogenetic analysis confirmed the morphological identification of all <em>P. perniciosus</em> specimens, grouping them into a single clade, with distinct subclades corresponding to the geographical origin. Haplotype analysis revealed 56 genetic variants, with the predominant haplotype represented by 37 specimens in Majorca and 40 in Barcelona. Significant genetic differentiation was observed between populations from Majorca and Barcelona (Fst = 0.78262; p < 0.00001), indicating limited gene flow. Nucleotide diversity was higher in Majorca (π ± SD = 0.0037 ± 0.00090) than in Barcelona (π ± SD = 0.0006 ± 0.00021). Majorcan specimens showed close genetic affinity to the Algerian and Tunisian populations (Fst = 0.02470; p > 0.05), while Barcelona specimens were more closely related to those of the South and West Iberian Peninsula (Fst = 0.51225; p < 0.00001).</p><p><strong>Conclusions:</strong> These findings indicate that geographic isolation and historical dispersal may have shaped the P. perniciosus genetic structure. The Balearic Sea appears to act as a significant barrier, restricting gene flow between island and Iberian mainland populations. The study supports the utility of COI in phylogeographic research and demonstrates how island-mainland comparisons can help reveal evolutionary processes in vector species.BioMed Central2026202620262026info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion34 p.application/pdfhttps://hdl.handle.net/2445/226396Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a:Parasites & Vectors, 2026cc-by (c) Chavez-Fisa, S. et al., 2026http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/2263962026-05-29T05:05:01Z
dc.title.none.fl_str_mv Genetic diversity of <em>Phlebotomus perniciosus</em>populations between insular and mainland regionsin the leishmaniasis-endemic westernMediterranean area
title Genetic diversity of <em>Phlebotomus perniciosus</em>populations between insular and mainland regionsin the leishmaniasis-endemic westernMediterranean area
spellingShingle Genetic diversity of <em>Phlebotomus perniciosus</em>populations between insular and mainland regionsin the leishmaniasis-endemic westernMediterranean area
Chavez-Fisa, Sarah
Illes Balears
Leishmania infantum
Leishmaniosi
Balearic Islands
Leishmania infantum
Leishmaniasis
title_short Genetic diversity of <em>Phlebotomus perniciosus</em>populations between insular and mainland regionsin the leishmaniasis-endemic westernMediterranean area
title_full Genetic diversity of <em>Phlebotomus perniciosus</em>populations between insular and mainland regionsin the leishmaniasis-endemic westernMediterranean area
title_fullStr Genetic diversity of <em>Phlebotomus perniciosus</em>populations between insular and mainland regionsin the leishmaniasis-endemic westernMediterranean area
title_full_unstemmed Genetic diversity of <em>Phlebotomus perniciosus</em>populations between insular and mainland regionsin the leishmaniasis-endemic westernMediterranean area
title_sort Genetic diversity of <em>Phlebotomus perniciosus</em>populations between insular and mainland regionsin the leishmaniasis-endemic westernMediterranean area
dc.creator.none.fl_str_mv Chavez-Fisa, Sarah
Roca Geronès, Xavier
Fisa Saladrigas, Roser
Riera Lizandra, Ma. Cristina
Alcover Amengual, Maria Magdalena
author Chavez-Fisa, Sarah
author_facet Chavez-Fisa, Sarah
Roca Geronès, Xavier
Fisa Saladrigas, Roser
Riera Lizandra, Ma. Cristina
Alcover Amengual, Maria Magdalena
author_role author
author2 Roca Geronès, Xavier
Fisa Saladrigas, Roser
Riera Lizandra, Ma. Cristina
Alcover Amengual, Maria Magdalena
author2_role author
author
author
author
dc.subject.none.fl_str_mv Illes Balears
Leishmania infantum
Leishmaniosi
Balearic Islands
Leishmania infantum
Leishmaniasis
topic Illes Balears
Leishmania infantum
Leishmaniosi
Balearic Islands
Leishmania infantum
Leishmaniasis
description Phlebotomus perniciosus</em> is the primary vector of <em>Leishmania infantum</em> in Spain, occurring in both continental and insular regions. This study investigates the genetic structure of <em>P. perniciosus</em> populations from Majorca (island) and Barcelona (mainland), two geographically close but ecologically distinct regions in the western Mediterranean.</p><p><strong>Methods: </strong>Mitochondrial cytochrome c oxidase subunit I (COI) gene sequences were analyzed from 167 <em>P. perniciosus</em> specimens, including 100 morphologically identified field-collected specimens from Majorca and Barcelona, supplemented with reference data from the South and West Iberian Peninsula and North Africa. Population differentiation was assessed using genetic diversity indices, Bayesian phylogenetic inference, analysis of molecular variance (AMOVA), pairwise Fst values and Nm estimates, haplotype networks, and a Mantel test.</p><p><strong>Results:</strong> Phylogenetic analysis confirmed the morphological identification of all <em>P. perniciosus</em> specimens, grouping them into a single clade, with distinct subclades corresponding to the geographical origin. Haplotype analysis revealed 56 genetic variants, with the predominant haplotype represented by 37 specimens in Majorca and 40 in Barcelona. Significant genetic differentiation was observed between populations from Majorca and Barcelona (Fst = 0.78262; p < 0.00001), indicating limited gene flow. Nucleotide diversity was higher in Majorca (π ± SD = 0.0037 ± 0.00090) than in Barcelona (π ± SD = 0.0006 ± 0.00021). Majorcan specimens showed close genetic affinity to the Algerian and Tunisian populations (Fst = 0.02470; p > 0.05), while Barcelona specimens were more closely related to those of the South and West Iberian Peninsula (Fst = 0.51225; p < 0.00001).</p><p><strong>Conclusions:</strong> These findings indicate that geographic isolation and historical dispersal may have shaped the P. perniciosus genetic structure. The Balearic Sea appears to act as a significant barrier, restricting gene flow between island and Iberian mainland populations. The study supports the utility of COI in phylogeographic research and demonstrates how island-mainland comparisons can help reveal evolutionary processes in vector species.
publishDate 2026
dc.date.none.fl_str_mv 2026
2026
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/226396
url https://hdl.handle.net/2445/226396
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a:
Parasites & Vectors, 2026
dc.rights.none.fl_str_mv cc-by (c) Chavez-Fisa, S. et al., 2026
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Chavez-Fisa, S. et al., 2026
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 34 p.
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869414959448850432
score 15,81155

Registros relacionados