Asymmetrical nucleosomal DNA signatures regulate transcriptional directionality.

[EN]Despite the symmetrical structure of nucleosomes, in vitro studies have shown that transcription proceeds with different efficiency depending on the orientation of the DNA sequence around them. However, it is unclear whether this functional asymmetry is present in vivo and whether it could regul...

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Detalles Bibliográficos
Autores: García Martínez, Alicia, Durán, Laura, Sánchez, Mar, González, Sara, Santamaría Vicente, Rodrigo, Antequera Márquez, Francisco
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Universidad de Salamanca (USAL)
Repositorio:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/154334
Acceso en línea:http://hdl.handle.net/10366/154334
Access Level:acceso abierto
Palabra clave:Nucleosomes
Transcription
Transcription directionality
Transcriptomics
Genomics
DNA Sequence analysis
DNA Nucleosome signature
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spelling Asymmetrical nucleosomal DNA signatures regulate transcriptional directionality.García Martínez, AliciaDurán, LauraSánchez, MarGonzález, SaraSantamaría Vicente, RodrigoAntequera Márquez, FranciscoNucleosomesTranscriptionTranscription directionalityTranscriptomicsGenomicsDNA Sequence analysisDNA Nucleosome signature[EN]Despite the symmetrical structure of nucleosomes, in vitro studies have shown that transcription proceeds with different efficiency depending on the orientation of the DNA sequence around them. However, it is unclear whether this functional asymmetry is present in vivo and whether it could regulate transcriptional directionality. Here, we report that the proximal and distal halves of nucleosomal DNA contribute differentially to nucleosome stability in the genome. In +1 nucleosomes, this asymmetry facilitates or hinders transcription depending on the orientation of its underlying DNA, and this difference is associated with an asymmetrical interaction between DNA and histones. These properties are encoded in the DNA signature of +1 nucleosomes, since its incorporation in the two orientations into downstream nucleosomes renders them asymmetrically accessible to MNase and inverts the balance between sense and antisense transcription. Altogether, our results show that nucleosomal DNA endows nucleosomes with asymmetrical properties that modulate the directionality of transcription.202420242023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10366/154334reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)InglésPID2020-118423GB-I00Atribución-NoComercial-CompartirIgual 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccessoai:gredos.usal.es:10366/1543342026-06-07T06:28:51Z
dc.title.none.fl_str_mv Asymmetrical nucleosomal DNA signatures regulate transcriptional directionality.
title Asymmetrical nucleosomal DNA signatures regulate transcriptional directionality.
spellingShingle Asymmetrical nucleosomal DNA signatures regulate transcriptional directionality.
García Martínez, Alicia
Nucleosomes
Transcription
Transcription directionality
Transcriptomics
Genomics
DNA Sequence analysis
DNA Nucleosome signature
title_short Asymmetrical nucleosomal DNA signatures regulate transcriptional directionality.
title_full Asymmetrical nucleosomal DNA signatures regulate transcriptional directionality.
title_fullStr Asymmetrical nucleosomal DNA signatures regulate transcriptional directionality.
title_full_unstemmed Asymmetrical nucleosomal DNA signatures regulate transcriptional directionality.
title_sort Asymmetrical nucleosomal DNA signatures regulate transcriptional directionality.
dc.creator.none.fl_str_mv García Martínez, Alicia
Durán, Laura
Sánchez, Mar
González, Sara
Santamaría Vicente, Rodrigo
Antequera Márquez, Francisco
author García Martínez, Alicia
author_facet García Martínez, Alicia
Durán, Laura
Sánchez, Mar
González, Sara
Santamaría Vicente, Rodrigo
Antequera Márquez, Francisco
author_role author
author2 Durán, Laura
Sánchez, Mar
González, Sara
Santamaría Vicente, Rodrigo
Antequera Márquez, Francisco
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Nucleosomes
Transcription
Transcription directionality
Transcriptomics
Genomics
DNA Sequence analysis
DNA Nucleosome signature
topic Nucleosomes
Transcription
Transcription directionality
Transcriptomics
Genomics
DNA Sequence analysis
DNA Nucleosome signature
description [EN]Despite the symmetrical structure of nucleosomes, in vitro studies have shown that transcription proceeds with different efficiency depending on the orientation of the DNA sequence around them. However, it is unclear whether this functional asymmetry is present in vivo and whether it could regulate transcriptional directionality. Here, we report that the proximal and distal halves of nucleosomal DNA contribute differentially to nucleosome stability in the genome. In +1 nucleosomes, this asymmetry facilitates or hinders transcription depending on the orientation of its underlying DNA, and this difference is associated with an asymmetrical interaction between DNA and histones. These properties are encoded in the DNA signature of +1 nucleosomes, since its incorporation in the two orientations into downstream nucleosomes renders them asymmetrically accessible to MNase and inverts the balance between sense and antisense transcription. Altogether, our results show that nucleosomal DNA endows nucleosomes with asymmetrical properties that modulate the directionality of transcription.
publishDate 2023
dc.date.none.fl_str_mv 2023
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10366/154334
url http://hdl.handle.net/10366/154334
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv PID2020-118423GB-I00
dc.rights.none.fl_str_mv Atribución-NoComercial-CompartirIgual 4.0 Internacional
http://creativecommons.org/licenses/by-nc-sa/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial-CompartirIgual 4.0 Internacional
http://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca
instname:Universidad de Salamanca (USAL)
instname_str Universidad de Salamanca (USAL)
reponame_str GREDOS. Repositorio Institucional de la Universidad de Salamanca
collection GREDOS. Repositorio Institucional de la Universidad de Salamanca
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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