Data about mammalian cell interaction promoted by a recombinan calreticulin
Set of data investigating the cell interactivity of a recombinant calreticulin. 1. Description of methods used for collection-generation of data: In vivo biodistribution: n this study, nanoparticle biodistribution was examined in an Acute Myeloid Leukemia mouse model. After injecting THP-1 cells, tu...
| Autores: | , , , , , , , |
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| Tipo de recurso: | conjunto de datos |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Consorci de Serveis Universitaris de Catalunya (CSUC) |
| Repositorio: | CORA.Repositori de Dades de Recerca |
| OAI Identifier: | oai:dnet:cora.rdr____::3164af34e587273376dbdb465e66d798 |
| Acceso en línea: | https://doi.org/10.34810/DATA1055 |
| Access Level: | acceso abierto |
| Palabra clave: | Medicine, Health and Life Sciences Recombinant proteins Protein materials Nanoparticles Drug delivery Cell targeting |
| Sumario: | Set of data investigating the cell interactivity of a recombinant calreticulin. 1. Description of methods used for collection-generation of data: In vivo biodistribution: n this study, nanoparticle biodistribution was examined in an Acute Myeloid Leukemia mouse model. After injecting THP-1 cells, tumor growth was monitored, and when tumors reached 300-400 mm^3, mice received a single intravenous dose (buffer, 200 mg GFP-H6, or 200 mg V1-GFP-pcCRT-H6). Mice were euthanized at 1, 5, and 24 hours post-injection. Fluorescence intensity, indicating nanoparticle accumulation, was measured in tissues using the IVIS Spectrum 200 Imaging System. The results were determined by subtracting autofluorescence observed in control mice injected with buffer. Confocal microscopy: In confocal microscopy, HeLa cells on MatTek plates were exposed to proteins of interest in OptiProTM medium for 2 to 24 hours. Cell nuclei were labeled with Hoechst 33342, and plasma membranes were stained with CellMask™ Deep Red. Imaging was done with a TCS SP5 Leica Spectral confocal microscope. Excitation used 405 nm (Hoechst), 488 nm (GFP), and 633 nm (CellMask™) lasers. Detection voltages were optimized to avoid cross-talk. The rest of methods are standard and well described elsewhere: https://doi.org/10.1016/j.biopha.2023.114976 2. Methods for processing the data: Data processing details are standard and well described elsewhere: https://doi.org/10.1016/j.biopha.2023.114976 3. Instrument- or software- specific information needed to interpret the data: Leica Application Suite X, version 3.5.7, available at: https://www.leica-microsystems.com/es/productos/software-de-microscopia/p/leica-las-x-ls/downloads/ GraphPad Prism, version 8.0.2, available at: https://www.graphpad.com/ Cytexpert, version 2.4, available at: https://www.beckman.es/flow-cytometry/research-flow-cytometers/cytoflex/software?country=ES 4. Instruments, calibration and standards information: EmulsiFlex-C5 system (Avestin) ÄKTA pure system (Cytiva) Zetasizer Pro (Malvern Analytical) IVIS Spectrum 200 Imaging System (PerkinElmer) TCS SP5 Leica Spectral confocal microscope (Leica Microsystems) CytoFLEX Flow Cytometer (Beckman Coulter) 5. Environmental or experimental conditions: Data processing and figure creation were performed in Windows 10 OS. |
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