Enhancing Nutritional and Functional Properties of Broccoli Leaves Through Selenium Biofortification

Selenium (Se) biofortification is a promising agronomic strategy to enhance the dietary intake of this essential micronutrient while simultaneously adding value to agricultural by-products like Brassica oleracea L. var. italica leaves. This study evaluated the effects of foliar Se biofortification o...

Descripción completa

Detalles Bibliográficos
Autores: Buttarelli, Melina Soledad, Céccoli, Gabriel|||0000-0003-2512-2840, Trod, Betiana Soledad, Stoffel, María Micaela, Simonutti, Marianela|||0000-0001-7953-319X, Bouzo, Carlos Alberto, Turowski, Valeria Rosana, Perez, Adrián Alejandro, Llugany, Mercè|||0000-0002-9940-0383, Sánchez-Martín, María-Jesús|||0000-0003-1678-6055, Daurelio, Lucas Damián, Guevara, María Gabriela|||0000-0002-6481-4711, Muñoz, Fernando Felipe|||0000-0001-6374-6676
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:310303
Acceso en línea:https://ddd.uab.cat/record/310303
https://dx.doi.org/urn:doi:10.3390/agronomy15020389
Access Level:acceso abierto
Palabra clave:Brassicaceae
Selenate
Selenite
By-products
Fusarium solani
Descripción
Sumario:Selenium (Se) biofortification is a promising agronomic strategy to enhance the dietary intake of this essential micronutrient while simultaneously adding value to agricultural by-products like Brassica oleracea L. var. italica leaves. This study evaluated the effects of foliar Se biofortification on a fresh market broccoli cultivar ('Belstar') using selenite and selenate (1 and 2 mM). Growth performance, biochemical properties, nutraceutical quality, and phytohormone profiles of broccoli leaves were analyzed, highlighting their potential as functional by-products. Multivariate analysis revealed that 2 mM selenite application was the most effective treatment, significantly improving several parameters. Selenium biofortification with 2 mM selenite increased essential nutrient content, including Se, Ca, S, Fe, Mn, Mg, and Mo. It also enhanced the soluble protein content (+2.2-fold), phenolic compounds (+1.5-fold), and total antioxidant capacity (+1.4-fold) compared to control plants. In this sense, the nutraceutical quality of broccoli leaves was markedly improved, supporting their use as a source of bioactive ingredients. Additionally, to assess practical applications, water-extracted Se-enriched broccoli leaves demonstrated antifungal activity against the plant pathogen Fusarium solani, attributed to Se-induced alterations in phytohormone profiles. These findings suggest that Se-biofortified broccoli leaves can serve as a sustainable source of essential nutrients and bioactive compounds for the food industry. Furthermore, their antifungal properties position them as potential eco-friendly biopesticides to combat plant pathogenic fungi, thereby promoting sustainable agriculture.