Evidence that heparin but not hirudin reduces PAI-1 expression in cultured human endothelial cells

Heparin and other antithrombotic drugs besides their anticoagulant action could have a profibrinolytic effect. We have analyzed the effect of unfractionated heparin (UFH) and hirudin on PAI-1 gene expression in human umbilical vein endothelial cells (HUVEC). Cells were stimulated with UFH (1 and 10...

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Detalles Bibliográficos
Autores: Orbe-Lopategui, J. (Josune)|||/items/033ea7ef-1eff-418a-9fc4-1e2d3498d726, Montes, R. (Ramón)|||/items/8f33031a-c4c2-48c3-beb6-0177898ba38d, Zabalegui, N. (Natalia)|||/items/f09ce17d-3127-4609-881f-c8ae3c244607, Perez-Ruiz, A. (Ana)|||/items/fbeb9d68-51d3-4c79-83dd-80a487d8b5a4, Páramo-Fernández, J.A. (José Antonio)|||/items/8c56baa6-2a43-4836-b91b-4eed15be3c96
Tipo de recurso: artículo
Fecha de publicación:1999
País:España
Institución:Universidad de Navarra
Repositorio:Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:inglés
OAI Identifier:oai:dadun.unav.edu:10171/22262
Acceso en línea:https://hdl.handle.net/10171/22262
Access Level:acceso abierto
Palabra clave:Heparin
mRNA
Hirudin
Endotoxin
PAI-1
HUVEC
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spelling Evidence that heparin but not hirudin reduces PAI-1 expression in cultured human endothelial cellsOrbe-Lopategui, J. (Josune)|||/items/033ea7ef-1eff-418a-9fc4-1e2d3498d726Montes, R. (Ramón)|||/items/8f33031a-c4c2-48c3-beb6-0177898ba38dZabalegui, N. (Natalia)|||/items/f09ce17d-3127-4609-881f-c8ae3c244607Perez-Ruiz, A. (Ana)|||/items/fbeb9d68-51d3-4c79-83dd-80a487d8b5a4Páramo-Fernández, J.A. (José Antonio)|||/items/8c56baa6-2a43-4836-b91b-4eed15be3c96HeparinmRNAHirudinEndotoxinPAI-1HUVECHeparin and other antithrombotic drugs besides their anticoagulant action could have a profibrinolytic effect. We have analyzed the effect of unfractionated heparin (UFH) and hirudin on PAI-1 gene expression in human umbilical vein endothelial cells (HUVEC). Cells were stimulated with UFH (1 and 10 IU/ml) and hirudin (20 and 100 TIU/ml). Samples were obtained before and 2, 6, and 24 hours after stimulation. mRNA analysis was conducted by reverse transcription followed by polymerase chain reaction, and PAI-1 antigen was determined by ELISA. Addition of UFH (10 IU/ml) to HUVEC resulted in a decrease of PAI-1 mRNA at 6 hours (40% reduction) and 24 hours (60% reduction) and PAI-1 antigen. Hirudin, however, did not modify significantly the PAI-1 mRNA nor the inhibitor secretion. The addition of UFH (10 or 100 IU/ml) to endotoxin-stimulated HUVEC also reduced the increased PAI-1 mRNA and antigen secretion (45%), whereas no effect could be observed with hirudin. Our results suggest that UFH, but not hirudin, by reducing the endothelial expression of PAI-1 might have a profibrinolytic effect.ElsevierDadun. Depósito Académico Digital Universidad de Navarra20122012-05-2819991999-01-0119991999-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/22262reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/222622026-06-21T12:47:57Z
dc.title.none.fl_str_mv Evidence that heparin but not hirudin reduces PAI-1 expression in cultured human endothelial cells
title Evidence that heparin but not hirudin reduces PAI-1 expression in cultured human endothelial cells
spellingShingle Evidence that heparin but not hirudin reduces PAI-1 expression in cultured human endothelial cells
Orbe-Lopategui, J. (Josune)|||/items/033ea7ef-1eff-418a-9fc4-1e2d3498d726
Heparin
mRNA
Hirudin
Endotoxin
PAI-1
HUVEC
title_short Evidence that heparin but not hirudin reduces PAI-1 expression in cultured human endothelial cells
title_full Evidence that heparin but not hirudin reduces PAI-1 expression in cultured human endothelial cells
title_fullStr Evidence that heparin but not hirudin reduces PAI-1 expression in cultured human endothelial cells
title_full_unstemmed Evidence that heparin but not hirudin reduces PAI-1 expression in cultured human endothelial cells
title_sort Evidence that heparin but not hirudin reduces PAI-1 expression in cultured human endothelial cells
dc.creator.none.fl_str_mv Orbe-Lopategui, J. (Josune)|||/items/033ea7ef-1eff-418a-9fc4-1e2d3498d726
Montes, R. (Ramón)|||/items/8f33031a-c4c2-48c3-beb6-0177898ba38d
Zabalegui, N. (Natalia)|||/items/f09ce17d-3127-4609-881f-c8ae3c244607
Perez-Ruiz, A. (Ana)|||/items/fbeb9d68-51d3-4c79-83dd-80a487d8b5a4
Páramo-Fernández, J.A. (José Antonio)|||/items/8c56baa6-2a43-4836-b91b-4eed15be3c96
author Orbe-Lopategui, J. (Josune)|||/items/033ea7ef-1eff-418a-9fc4-1e2d3498d726
author_facet Orbe-Lopategui, J. (Josune)|||/items/033ea7ef-1eff-418a-9fc4-1e2d3498d726
Montes, R. (Ramón)|||/items/8f33031a-c4c2-48c3-beb6-0177898ba38d
Zabalegui, N. (Natalia)|||/items/f09ce17d-3127-4609-881f-c8ae3c244607
Perez-Ruiz, A. (Ana)|||/items/fbeb9d68-51d3-4c79-83dd-80a487d8b5a4
Páramo-Fernández, J.A. (José Antonio)|||/items/8c56baa6-2a43-4836-b91b-4eed15be3c96
author_role author
author2 Montes, R. (Ramón)|||/items/8f33031a-c4c2-48c3-beb6-0177898ba38d
Zabalegui, N. (Natalia)|||/items/f09ce17d-3127-4609-881f-c8ae3c244607
Perez-Ruiz, A. (Ana)|||/items/fbeb9d68-51d3-4c79-83dd-80a487d8b5a4
Páramo-Fernández, J.A. (José Antonio)|||/items/8c56baa6-2a43-4836-b91b-4eed15be3c96
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Dadun. Depósito Académico Digital Universidad de Navarra
dc.subject.none.fl_str_mv Heparin
mRNA
Hirudin
Endotoxin
PAI-1
HUVEC
topic Heparin
mRNA
Hirudin
Endotoxin
PAI-1
HUVEC
description Heparin and other antithrombotic drugs besides their anticoagulant action could have a profibrinolytic effect. We have analyzed the effect of unfractionated heparin (UFH) and hirudin on PAI-1 gene expression in human umbilical vein endothelial cells (HUVEC). Cells were stimulated with UFH (1 and 10 IU/ml) and hirudin (20 and 100 TIU/ml). Samples were obtained before and 2, 6, and 24 hours after stimulation. mRNA analysis was conducted by reverse transcription followed by polymerase chain reaction, and PAI-1 antigen was determined by ELISA. Addition of UFH (10 IU/ml) to HUVEC resulted in a decrease of PAI-1 mRNA at 6 hours (40% reduction) and 24 hours (60% reduction) and PAI-1 antigen. Hirudin, however, did not modify significantly the PAI-1 mRNA nor the inhibitor secretion. The addition of UFH (10 or 100 IU/ml) to endotoxin-stimulated HUVEC also reduced the increased PAI-1 mRNA and antigen secretion (45%), whereas no effect could be observed with hirudin. Our results suggest that UFH, but not hirudin, by reducing the endothelial expression of PAI-1 might have a profibrinolytic effect.
publishDate 1999
dc.date.none.fl_str_mv 1999
1999-01-01
1999
1999-01-01
2012
2012-05-28
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10171/22262
url https://hdl.handle.net/10171/22262
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra
instname:Universidad de Navarra
instname_str Universidad de Navarra
reponame_str Dadun. Depósito Académico Digital de la Universidad de Navarra
collection Dadun. Depósito Académico Digital de la Universidad de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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