Engineering the steroid hydroxylating system from Cochliobolus lunatus in Mycolicibacterium smegmatis

13 p.-5 fig.-1 tab.

Detalles Bibliográficos
Autores: Felpeto-Santero, Carmen, Galán, Beatriz, García, José Luis
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/262476
Acceso en línea:http://hdl.handle.net/10261/262476
Access Level:acceso abierto
Palabra clave:Steroids
14α-hydroxylation
Mycocilibacterium smegmatis
Steroids biotechnology
Steroids functionalization
Hydroxylated steroidal synthons
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spelling Engineering the steroid hydroxylating system from Cochliobolus lunatus in Mycolicibacterium smegmatisFelpeto-Santero, CarmenGalán, BeatrizGarcía, José LuisSteroids14α-hydroxylationMycocilibacterium smegmatisSteroids biotechnologySteroids functionalizationHydroxylated steroidal synthons13 p.-5 fig.-1 tab.14α-hydroxylated steroids are starting materials for the synthesis of contraceptive and anti-inflammatory compounds in the steroid industry. A synthetic bacterial operon containing the cytochrome P450 CYP103168 and the reductase CPR64795 of the fungus Cochlioboluslunatus able to hydroxylate steroids has been engineered into a shuttle plasmid named pMVFAN. This plasmid was used to transform two mutants of Mycolicibacterium smegmatis named MS6039-5941 and MS6039 that accumulate 4-androstene-3,17-dione (AD), and 1,4-androstadiene-3,17-dione (ADD), respectively. The recombinant mutants MS6039-5941 (pMVFAN) and MS6039 (pMVFAN) were able to efficiently express the hydroxylating CYP system of C.lunatus and produced in high yields 14αOH-AD and 14αOH-ADD, respectively, directly from cholesterol and phytosterols in a single fermentation step. These results open a new avenue for producing at industrial scale these and other hydroxylated steroidal synthons by transforming with this synthetic operon other Mycolicibacterium strains currently used for the commercial production of steroidal synthons from phytosterols as feedstock.This work was supported by grants from the Ministry of Science and Innovation (BFU2006-15214-C03-01, BFU2009-11545-C03-03) and Ministry of Economy and Competitiveness (BIO2012-39695-C02-01).Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Ciencia e Innovación (España)Ministerio de Economía y Competitividad (España)Galán, Beatriz [0000-0002-2596-6034]García, José Luis [0000-0002-9238-2485]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/262476reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MEC//BFU2006-15214-C03-01info:eu-repo/grantAgreement/MICINN//BFU2009-11545-C03-03info:eu-repo/grantAgreement/MINECO//BIO2012-39695-C02-01https://doi.org/10.3390/microorganisms9071499Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2624762026-05-22T06:33:51Z
dc.title.none.fl_str_mv Engineering the steroid hydroxylating system from Cochliobolus lunatus in Mycolicibacterium smegmatis
title Engineering the steroid hydroxylating system from Cochliobolus lunatus in Mycolicibacterium smegmatis
spellingShingle Engineering the steroid hydroxylating system from Cochliobolus lunatus in Mycolicibacterium smegmatis
Felpeto-Santero, Carmen
Steroids
14α-hydroxylation
Mycocilibacterium smegmatis
Steroids biotechnology
Steroids functionalization
Hydroxylated steroidal synthons
title_short Engineering the steroid hydroxylating system from Cochliobolus lunatus in Mycolicibacterium smegmatis
title_full Engineering the steroid hydroxylating system from Cochliobolus lunatus in Mycolicibacterium smegmatis
title_fullStr Engineering the steroid hydroxylating system from Cochliobolus lunatus in Mycolicibacterium smegmatis
title_full_unstemmed Engineering the steroid hydroxylating system from Cochliobolus lunatus in Mycolicibacterium smegmatis
title_sort Engineering the steroid hydroxylating system from Cochliobolus lunatus in Mycolicibacterium smegmatis
dc.creator.none.fl_str_mv Felpeto-Santero, Carmen
Galán, Beatriz
García, José Luis
author Felpeto-Santero, Carmen
author_facet Felpeto-Santero, Carmen
Galán, Beatriz
García, José Luis
author_role author
author2 Galán, Beatriz
García, José Luis
author2_role author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Ministerio de Economía y Competitividad (España)
Galán, Beatriz [0000-0002-2596-6034]
García, José Luis [0000-0002-9238-2485]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Steroids
14α-hydroxylation
Mycocilibacterium smegmatis
Steroids biotechnology
Steroids functionalization
Hydroxylated steroidal synthons
topic Steroids
14α-hydroxylation
Mycocilibacterium smegmatis
Steroids biotechnology
Steroids functionalization
Hydroxylated steroidal synthons
description 13 p.-5 fig.-1 tab.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/262476
url http://hdl.handle.net/10261/262476
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MEC//BFU2006-15214-C03-01
info:eu-repo/grantAgreement/MICINN//BFU2009-11545-C03-03
info:eu-repo/grantAgreement/MINECO//BIO2012-39695-C02-01
https://doi.org/10.3390/microorganisms9071499
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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