Mechanisms of Tolerance and Resistance to Chlorhexidine in Clinical Strains of Klebsiella pneumoniae Producers of Carbapenemase: Role of New Type II Toxin-Antitoxin System, PemIK

This article belongs to the Special Issue Toxin-Antitoxin Systems in Pathogenic Bacteria.

Detalhes bibliográficos
Autores: Bleriot, Inés, Blasco, Lucía, Delgado-Valverde, Mercedes, Gual-de-Torrella, Ana, Ambroa, Antón, Fernández-García, Laura, López, María, Oteo-Iglesias, Jesús, Wood, Thomas K., Pascual, Álvaro, Bou, Germán, Fernández-Cuenca, Felipe, Tomás, María
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/235280
Acesso em linha:http://hdl.handle.net/10261/235280
Access Level:acceso abierto
Palavra-chave:Tolerance
Persistence
Cross-resistance
Toxin-antitoxin system
PemI/PemK
Klebsiella pneumoniae
id ES_9f8b171b477d0ce6a1e97e5efd2e98f2
oai_identifier_str oai:digital.csic.es:10261/235280
network_acronym_str ES
network_name_str España
repository_id_str
spelling Mechanisms of Tolerance and Resistance to Chlorhexidine in Clinical Strains of Klebsiella pneumoniae Producers of Carbapenemase: Role of New Type II Toxin-Antitoxin System, PemIKBleriot, InésBlasco, LucíaDelgado-Valverde, MercedesGual-de-Torrella, AnaAmbroa, AntónFernández-García, LauraLópez, MaríaOteo-Iglesias, JesúsWood, Thomas K.Pascual, ÁlvaroBou, GermánFernández-Cuenca, FelipeTomás, MaríaTolerancePersistenceCross-resistanceToxin-antitoxin systemPemI/PemKKlebsiella pneumoniaeThis article belongs to the Special Issue Toxin-Antitoxin Systems in Pathogenic Bacteria.Although the failure of antibiotic treatment is normally attributed to resistance, tolerance and persistence display a significant role in the lack of response to antibiotics. Due to the fact that several nosocomial pathogens show a high level of tolerance and/or resistance to chlorhexidine, in this study we analyzed the molecular mechanisms associated with chlorhexidine adaptation in two clinical strains of Klebsiella pneumoniae by phenotypic and transcriptomic studies. These two strains belong to ST258-KPC3 (high-risk clone carrying β-lactamase KPC3) and ST846-OXA48 (low-risk clone carrying β-lactamase OXA48). Our results showed that the K. pneumoniae ST258-KPC3CA and ST846-OXA48CA strains exhibited a different behavior under chlorhexidine (CHLX) pressure, adapting to this biocide through resistance and tolerance mechanisms, respectively. Furthermore, the appearance of cross-resistance to colistin was observed in the ST846-OXA48CA strain (tolerant to CHLX), using the broth microdilution method. Interestingly, this ST846-OXA48CA isolate contained a plasmid that encodes a novel type II toxin/antitoxin (TA) system, PemI/PemK. We characterized this PemI/PemK TA system by cloning both genes into the IPTG-inducible pCA24N plasmid, and found their role in persistence and biofilm formation. Accordingly, the ST846-OXA48CA strain showed a persistence biphasic curve in the presence of a chlorhexidine-imipenem combination, and these results were confirmed by the enzymatic assay (WST-1).This study was funded by grants PI16/01163 and PI19/00878 awarded to M. Tomás within the State Plan for R+D+I 2013–2016 (National Plan for Scientific Research, Technological Development and Innovation 2008–2011) and co-financed by the ISCIII-Deputy General Directorate for Evaluation and Promotion of Research - European Regional Development Fund “A way of Making Europe” and Instituto de Salud Carlos III FEDER, Spanish Network for the Research in Infectious Diseases (REIPI, RD16/0016/0001, RD16/CIII/0004/0002 and RD16/0016/0006) and by the Study Group on Mechanisms of Action and Resistance to Antimicrobials, GEMARA (SEIMC, http://www.seimc.org/).Multidisciplinary Digital Publishing InstituteInstituto de Salud Carlos IIIEuropean CommissionRed Española de Investigación en Patología InfecciosaSociedad Española de Enfermedades Infecciosas y Microbiología ClínicaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2021202120202021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/235280reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://doi.org/10.3390/toxins12090566Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2352802026-05-22T06:33:51Z
dc.title.none.fl_str_mv Mechanisms of Tolerance and Resistance to Chlorhexidine in Clinical Strains of Klebsiella pneumoniae Producers of Carbapenemase: Role of New Type II Toxin-Antitoxin System, PemIK
title Mechanisms of Tolerance and Resistance to Chlorhexidine in Clinical Strains of Klebsiella pneumoniae Producers of Carbapenemase: Role of New Type II Toxin-Antitoxin System, PemIK
spellingShingle Mechanisms of Tolerance and Resistance to Chlorhexidine in Clinical Strains of Klebsiella pneumoniae Producers of Carbapenemase: Role of New Type II Toxin-Antitoxin System, PemIK
Bleriot, Inés
Tolerance
Persistence
Cross-resistance
Toxin-antitoxin system
PemI/PemK
Klebsiella pneumoniae
title_short Mechanisms of Tolerance and Resistance to Chlorhexidine in Clinical Strains of Klebsiella pneumoniae Producers of Carbapenemase: Role of New Type II Toxin-Antitoxin System, PemIK
title_full Mechanisms of Tolerance and Resistance to Chlorhexidine in Clinical Strains of Klebsiella pneumoniae Producers of Carbapenemase: Role of New Type II Toxin-Antitoxin System, PemIK
title_fullStr Mechanisms of Tolerance and Resistance to Chlorhexidine in Clinical Strains of Klebsiella pneumoniae Producers of Carbapenemase: Role of New Type II Toxin-Antitoxin System, PemIK
title_full_unstemmed Mechanisms of Tolerance and Resistance to Chlorhexidine in Clinical Strains of Klebsiella pneumoniae Producers of Carbapenemase: Role of New Type II Toxin-Antitoxin System, PemIK
title_sort Mechanisms of Tolerance and Resistance to Chlorhexidine in Clinical Strains of Klebsiella pneumoniae Producers of Carbapenemase: Role of New Type II Toxin-Antitoxin System, PemIK
dc.creator.none.fl_str_mv Bleriot, Inés
Blasco, Lucía
Delgado-Valverde, Mercedes
Gual-de-Torrella, Ana
Ambroa, Antón
Fernández-García, Laura
López, María
Oteo-Iglesias, Jesús
Wood, Thomas K.
Pascual, Álvaro
Bou, Germán
Fernández-Cuenca, Felipe
Tomás, María
author Bleriot, Inés
author_facet Bleriot, Inés
Blasco, Lucía
Delgado-Valverde, Mercedes
Gual-de-Torrella, Ana
Ambroa, Antón
Fernández-García, Laura
López, María
Oteo-Iglesias, Jesús
Wood, Thomas K.
Pascual, Álvaro
Bou, Germán
Fernández-Cuenca, Felipe
Tomás, María
author_role author
author2 Blasco, Lucía
Delgado-Valverde, Mercedes
Gual-de-Torrella, Ana
Ambroa, Antón
Fernández-García, Laura
López, María
Oteo-Iglesias, Jesús
Wood, Thomas K.
Pascual, Álvaro
Bou, Germán
Fernández-Cuenca, Felipe
Tomás, María
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Salud Carlos III
European Commission
Red Española de Investigación en Patología Infecciosa
Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Tolerance
Persistence
Cross-resistance
Toxin-antitoxin system
PemI/PemK
Klebsiella pneumoniae
topic Tolerance
Persistence
Cross-resistance
Toxin-antitoxin system
PemI/PemK
Klebsiella pneumoniae
description This article belongs to the Special Issue Toxin-Antitoxin Systems in Pathogenic Bacteria.
publishDate 2020
dc.date.none.fl_str_mv 2020
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/235280
url http://hdl.handle.net/10261/235280
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://doi.org/10.3390/toxins12090566
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869414937138298880
score 15,811543

Registos relacionados