Use of mouse models to establish genotype-phenotype correlations in Williams-Beuren syndrome

Williams-Beuren syndrome (WBS) is a neurodevelopmental disorder caused by the common deletion of 26-28 contiguous genes in the 7q11.23 region, which poses difficulties to the establishment of genotype-phenotype correlations. The use of mouse models would broader the knowledge of the syndrome, the ro...

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Detalles Bibliográficos
Autor: Segura Puimedon, Maria
Tipo de recurso: tesis doctoral
Estado:Versión publicada
Fecha de publicación:2012
País:España
Institución:CBUC, CESCA
Repositorio:TDR. Tesis Doctorales en Red
OAI Identifier:oai:www.tdx.cat:10803/101408
Acceso en línea:http://hdl.handle.net/10803/101408
Access Level:acceso abierto
Palabra clave:Williams-Beuren syndrome
Mouse models
Transcriptome profile
Pathway enrichment
Binding motif
Cardiovascular phenotype
General transcription factor II-I (Gtf2i)
Neutrophil cytosolic factor 1 (Ncf1)
Síndrome de Williams-Beuren
Models de ratolí
Perfil transcriptòmic
Enriquiment de vies moleculars
Motiu d’unió
Fenotip cardiovascular
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Descripción
Sumario:Williams-Beuren syndrome (WBS) is a neurodevelopmental disorder caused by the common deletion of 26-28 contiguous genes in the 7q11.23 region, which poses difficulties to the establishment of genotype-phenotype correlations. The use of mouse models would broader the knowledge of the syndrome, the role of deleted genes, affected pathways and possible treatments. In this thesis project, several mouse models, tissues and cells have been used to define the phenotypes at different levels, the deregulated genes and pathways and to discover modifying elements and novel treatments for the cardiovascular phenotype. In addition, a new binding motif has been described for Gtf2i, a deleted gene encoding a transcription factor with a major role in WB, providing new target genes from deregulated pathways. The obtained results reveal the essential role of mouse models for the study of Williams-Beuren syndrome and provide new treatments options and affected pathways and genes which could be future treatment targets.