The transposon Galileo generates natural chromosomal inversions in drosophila by ectopic recombination

Background: transposable elements (TEs) are responsible for the generation of chromosomal inversions in several groups of organisms. However, in Drosophila and other Dipterans, where inversions are abundant both as intraspecific polymorphisms and interspecific fixed differences, the evidence for a r...

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Autores: Delprat Obeaga, Alejandra|||0000-0002-5187-802X, Negre de Bofarull, Bárbara|||0000-0002-7333-3979, Puig Font, Marta|||0000-0002-4439-6372, Ruiz, Alfredo|||0000-0002-3605-5922
Tipo de recurso: artículo
Fecha de publicación:2009
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:109356
Acceso en línea:https://ddd.uab.cat/record/109356
https://dx.doi.org/urn:doi:10.1371/journal.pone.0007883
Access Level:acceso abierto
Palabra clave:Cromosomes
Transposons
Genomes
Transposon Galileo
Drosophila
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spelling The transposon Galileo generates natural chromosomal inversions in drosophila by ectopic recombinationDelprat Obeaga, Alejandra|||0000-0002-5187-802XNegre de Bofarull, Bárbara|||0000-0002-7333-3979Puig Font, Marta|||0000-0002-4439-6372Ruiz, Alfredo|||0000-0002-3605-5922CromosomesTransposonsGenomesTransposon GalileoDrosophilaBackground: transposable elements (TEs) are responsible for the generation of chromosomal inversions in several groups of organisms. However, in Drosophila and other Dipterans, where inversions are abundant both as intraspecific polymorphisms and interspecific fixed differences, the evidence for a role of TEs is scarce. Previous work revealed that the transposon Galileo was involved in the generation of two polymorphic inversions of Drosophila buzzatii. Methodology/Principal Findings: to assess the impact of TEs in Drosophila chromosomal evolution and shed light on the mechanism involved, we isolated and sequenced the two breakpoints of another widespread polymorphic inversion from D. buzzatii, 2z3. In the non inverted chromosome, the 2z3 distal breakpoint was located between genes CG2046 and CG10326 whereas the proximal breakpoint lies between two novel genes that we have named Dlh and Mdp. In the inverted chromosome, the analysis of the breakpoint sequences revealed relatively large insertions (2,870-bp and 4,786-bp long) including two copies of the transposon Galileo (subfamily Newton), one at each breakpoint, plus several other TEs. The two Galileo copies: (i) are inserted in opposite orientation; (ii) present exchanged target site duplications; and (iii) are both chimeric. Conclusions/Significance: our observations provide the best evidence gathered so far for the role of TEs in the generation of Drosophila inversions. In addition, they show unequivocally that ectopic recombination is the causative mechanism. The fact that the three polymorphic D. buzzatii inversions investigated so far were generated by the same transposon family is remarkable and is conceivably due to Galileo's unusual structure and current (or recent) transpositional activity. 22009-01-0120092009-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/109356https://dx.doi.org/urn:doi:10.1371/journal.pone.0007883reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/2.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:1093562026-06-06T12:50:31Z
dc.title.none.fl_str_mv The transposon Galileo generates natural chromosomal inversions in drosophila by ectopic recombination
title The transposon Galileo generates natural chromosomal inversions in drosophila by ectopic recombination
spellingShingle The transposon Galileo generates natural chromosomal inversions in drosophila by ectopic recombination
Delprat Obeaga, Alejandra|||0000-0002-5187-802X
Cromosomes
Transposons
Genomes
Transposon Galileo
Drosophila
title_short The transposon Galileo generates natural chromosomal inversions in drosophila by ectopic recombination
title_full The transposon Galileo generates natural chromosomal inversions in drosophila by ectopic recombination
title_fullStr The transposon Galileo generates natural chromosomal inversions in drosophila by ectopic recombination
title_full_unstemmed The transposon Galileo generates natural chromosomal inversions in drosophila by ectopic recombination
title_sort The transposon Galileo generates natural chromosomal inversions in drosophila by ectopic recombination
dc.creator.none.fl_str_mv Delprat Obeaga, Alejandra|||0000-0002-5187-802X
Negre de Bofarull, Bárbara|||0000-0002-7333-3979
Puig Font, Marta|||0000-0002-4439-6372
Ruiz, Alfredo|||0000-0002-3605-5922
author Delprat Obeaga, Alejandra|||0000-0002-5187-802X
author_facet Delprat Obeaga, Alejandra|||0000-0002-5187-802X
Negre de Bofarull, Bárbara|||0000-0002-7333-3979
Puig Font, Marta|||0000-0002-4439-6372
Ruiz, Alfredo|||0000-0002-3605-5922
author_role author
author2 Negre de Bofarull, Bárbara|||0000-0002-7333-3979
Puig Font, Marta|||0000-0002-4439-6372
Ruiz, Alfredo|||0000-0002-3605-5922
author2_role author
author
author
dc.subject.none.fl_str_mv Cromosomes
Transposons
Genomes
Transposon Galileo
Drosophila
topic Cromosomes
Transposons
Genomes
Transposon Galileo
Drosophila
description Background: transposable elements (TEs) are responsible for the generation of chromosomal inversions in several groups of organisms. However, in Drosophila and other Dipterans, where inversions are abundant both as intraspecific polymorphisms and interspecific fixed differences, the evidence for a role of TEs is scarce. Previous work revealed that the transposon Galileo was involved in the generation of two polymorphic inversions of Drosophila buzzatii. Methodology/Principal Findings: to assess the impact of TEs in Drosophila chromosomal evolution and shed light on the mechanism involved, we isolated and sequenced the two breakpoints of another widespread polymorphic inversion from D. buzzatii, 2z3. In the non inverted chromosome, the 2z3 distal breakpoint was located between genes CG2046 and CG10326 whereas the proximal breakpoint lies between two novel genes that we have named Dlh and Mdp. In the inverted chromosome, the analysis of the breakpoint sequences revealed relatively large insertions (2,870-bp and 4,786-bp long) including two copies of the transposon Galileo (subfamily Newton), one at each breakpoint, plus several other TEs. The two Galileo copies: (i) are inserted in opposite orientation; (ii) present exchanged target site duplications; and (iii) are both chimeric. Conclusions/Significance: our observations provide the best evidence gathered so far for the role of TEs in the generation of Drosophila inversions. In addition, they show unequivocally that ectopic recombination is the causative mechanism. The fact that the three polymorphic D. buzzatii inversions investigated so far were generated by the same transposon family is remarkable and is conceivably due to Galileo's unusual structure and current (or recent) transpositional activity.
publishDate 2009
dc.date.none.fl_str_mv 2
2009-01-01
2009
2009-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/109356
https://dx.doi.org/urn:doi:10.1371/journal.pone.0007883
url https://ddd.uab.cat/record/109356
https://dx.doi.org/urn:doi:10.1371/journal.pone.0007883
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/2.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/2.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
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repository.mail.fl_str_mv
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