New transcriptomic insights in two RNAi wheat lines with the gliadins strongly down-regulated by two endosperm specific promoters
The gluten proteins of wheat grain are responsible for visco-elastic properties of flour, but they also trigger the immune-response of celiac disease. In this work, two low-gliadin RNA interference (RNAi) wheat lines that differ for the promoter driving the silencing (D-hordein and γ-gliadin promote...
| Autores: | , , |
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2022 |
| País: | España |
| Recursos: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/271757 |
| Acesso em linha: | http://hdl.handle.net/10261/271757 |
| Access Level: | acceso abierto |
| Palavra-chave: | Celiac disease Gliadin Protein compensation RNAi lines Triticum aestivum |
| Resumo: | The gluten proteins of wheat grain are responsible for visco-elastic properties of flour, but they also trigger the immune-response of celiac disease. In this work, two low-gliadin RNA interference (RNAi) wheat lines that differ for the promoter driving the silencing (D-hordein and γ-gliadin promoters for D783 and D793 lines, respectively), were characterized at transcriptomic, and protein fraction levels in the grain. Silencing of gliadins provides a readjustment in the grain protein fractions that also affects to the non-gluten proteins (NGP), which were increased in both RNAi lines. Determination of wheat gluten by means of the R5 monoclonal antibody also showed a strong reduction in the content of gluten in both RNAi lines. Moreover, fructans, an oligosaccharide linked with the development of non-celiac wheat sensitivity (NCWS) were also significantly decreased in RNAi lines. The down-regulation of gliadins fractions also impacts to other metabolic processes, particularly on carbohydrate metabolism, enzyme regulator activity and response to stress. Genes and transcription factors regulated by ABA were up-regulated, which could suggest the implication of this phytohormone on the stress response observed in the RNAi lines. |
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