Spiribacter roseus sp. nov., a moderately halophilic species of the genus Spiribacter from salterns
Four pink-pigmented, non-motile, Gram-staining-negative and moderately halophilic curved rods, designated strains SSL50T, SSL25, SSL97 and SSL4, were isolated from a saltern located in Isla Cristina, Huelva, South West Spain. Phylogenetic analyses based on 16S rRNA gene sequences showed that they we...
| Authors: | , , , |
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| Format: | article |
| Status: | Versión aceptada para publicación |
| Publication Date: | 2016 |
| Country: | España |
| Institution: | Universidad de Sevilla (US) |
| Repository: | idUS. Depósito de Investigación de la Universidad de Sevilla |
| OAI Identifier: | oai:idus.us.es:11441/164167 |
| Online Access: | https://hdl.handle.net/11441/164167 https://doi.org/10.1099/ijsem.0.001338 |
| Access Level: | Open access |
| Keyword: | Extremophiles Hypersaline Environments Moderately Halophilic Bacteria New Species Spiribacter roseus Taxonomy |
| Summary: | Four pink-pigmented, non-motile, Gram-staining-negative and moderately halophilic curved rods, designated strains SSL50T, SSL25, SSL97 and SSL4, were isolated from a saltern located in Isla Cristina, Huelva, South West Spain. Phylogenetic analyses based on 16S rRNA gene sequences showed that they were members of the genus Spiribacter, most closely related to Spiribacter curvatus UAH-SP71T(99.3-99.5 % sequence similarity) and Spiribacter salinus M19-40T(96.5-96.7 %). Other related species were Alkalilimnicola ehrlichii MLHE-1T(95.1-95.3 %), Arhodomonas recens RS91T(95.1-95.2 %) and Arhodomonas aquaeolei ATCC 49307T(95.0-95.1 %), all members of the family Ectothiorhodospiraceae. The major fatty acids are C18:1ω6c and/or C18:1ω7c, C16:0, and C12:0. The DNA G+C range is 64.0-66.3 mol%. The DNA-DNA hybridization between strains SSL50T, SSL25, SSL97, SSL4, and Spiribacter curvatus UAH-SP71Twere 37-49 %. The average nucleotide identity (ANIb) values between the strain SSL50Tgenome and the two other Spiribacter species, S. curvatus UAH-SP71Tand S. salinus M19-40Twas 82.4 % and 79.1 %, respectively, supporting the proposal of a new species of the genus Spiribacter. On the basis of the polyphasic analysis, the four new isolates are considered to represent a novel species of Spiribacter, for which the name Spiribacter roseus sp. nov. is proposed. The type strain is SSL50T(= CECT 9117T= IBRC-M 11076T). |
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