Estudios moleculares del gen "IytA" de "Streptococcus pneumoniae", y otros estreptococos relacionados, y su aplicación en epidemiología
Pneumococcus is a major cause of bacterial sepsis and the mean etiologic agent of acute otitis media, community−acquired pneumonia and non−epidemic bacterial meningitis (Bogaert et al., 2004; Wardlaw et al., 2006). According to World Health Organization (WHO), estimates of sepsis or pneumonia in neo...
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| Formato: | tesis doctoral |
| Fecha de publicación: | 2014 |
| País: | España |
| Recursos: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/118340 |
| Acesso em linha: | http://hdl.handle.net/10261/118340 |
| Access Level: | acceso abierto |
| Palavra-chave: | Neumococo Epidemiología Identificación clínica Gen galU |
| Resumo: | Pneumococcus is a major cause of bacterial sepsis and the mean etiologic agent of acute otitis media, community−acquired pneumonia and non−epidemic bacterial meningitis (Bogaert et al., 2004; Wardlaw et al., 2006). According to World Health Organization (WHO), estimates of sepsis or pneumonia in neonates and pneumonia in older children account for 29% of the 10.6 million yearly deaths in children young-er than 5 years, being pneumococcus the most common cause of severe pneumo-nia among children in developing countries as well as in children (http://www.unicef. org/Spanish/publications/files/Pneumonia_The_Forgotten_Killer_of_Children.pdf) and adults in Europe and the United States. Pneumococcal epidemiological studies are of great interest due to the high clinical incidence of this bacterium, the appear-ance of multiresistant strains and the need to study the efficacy of pneumococcal vaccines. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) are widely employed for pneumococcal typing. Although these techniques are powerful, they undergo some restrictions that prevent the implementation in the clinical setting since MLST is expensive whereas PFGE is time-consuming and comparison of results from different laboratories is far from trivial. Furthermore, MLST does not detect changes that may take place in other genomic regions and, as a consequence, there may have a great variability among strains corresponding to the same sequence type (Silva et al., 2006; Hiller et al., 2010; Croucher et al., 2011). Therefore, rapid, easy, low-cost, and reliable techniques are demanded. For this purpose, one of the main goals of the current study was to validate an alterna-tive typification procedure that involves sequencing other genes. |
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