A study by 1H NMR on the influence of some factors affecting lipid in vitro digestion

This article focuses on the impact of several experimental factors, including gastric acidification, intestinal transit time, presence of gastric lipase, sample/digestive fluids ratio, concentration and nature of the enzymes in intestinal juice, and bile concentration, on the extent of in vitro lipo...

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Detalles Bibliográficos
Autores: Nieva Echevarría, Bárbara, Goikoetxea Osés, Encarnación, Manzanos Arnaiz, María José, Guillén Loren, María Dolores
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/64628
Acceso en línea:http://hdl.handle.net/10810/64628
Access Level:acceso abierto
Palabra clave:1H NMR
lipolysis
in vitro digestion
fish lipids
bile salts
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spelling A study by 1H NMR on the influence of some factors affecting lipid in vitro digestionNieva Echevarría, BárbaraGoikoetxea Osés, EncarnaciónManzanos Arnaiz, María JoséGuillén Loren, María Dolores1H NMRlipolysisin vitro digestionfish lipidsbile saltsThis article focuses on the impact of several experimental factors, including gastric acidification, intestinal transit time, presence of gastric lipase, sample/digestive fluids ratio, concentration and nature of the enzymes in intestinal juice, and bile concentration, on the extent of in vitro lipolysis when using a static model that simulates human digestion processes in mouth, stomach and small intestine. The study was carried out by Proton Nuclear Magnetic Resonance (1H NMR). This technique provides a complete molecular picture of lipolysis, evidencing for the first time, whether preferential hydrolysis of certain glycerides over others occurs. A lipolysis degree similar to that reported in vivo was reached by varying certain variables within a physiological range; among them, bile concentration was found to be crucial. The holistic view of this 1H NMR study provides information of paramount importance to design sound in vitro digestion models to determine the bioaccessibility and bioavailability of lipophilic compounds.This work has been supported by the Spanish Ministry of Economy and Competitiveness (MINECO, AGL2012-36466, AGL2015-65450-R), by the Basque Government (EJ-GV, GIC10/85-IT-463-10) and by the Unit for Education and Research “Food Quality and Safety” (UPV/EHU-UFI-11/21). All authors participate in the COST Action FA1005-INFOGEST. B.N-E thanks UPV/EHU for a predoctoral contract.Elsevierj202420242016info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/64628reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoInglésinfo:eu-repo/grantAgreement/MINECO/AGL2012-36466/info:eu-repo/grantAgreement/MINECO/AGL2015-65450-R/https://doi.org/10.1016/j.foodchem.2016.05.021info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/3.0/es/Atribución-NoComercial-SinDerivadas 3.0 Españaoai:addi.ehu.eus:10810/646282026-06-18T09:23:17Z
dc.title.none.fl_str_mv A study by 1H NMR on the influence of some factors affecting lipid in vitro digestion
title A study by 1H NMR on the influence of some factors affecting lipid in vitro digestion
spellingShingle A study by 1H NMR on the influence of some factors affecting lipid in vitro digestion
Nieva Echevarría, Bárbara
1H NMR
lipolysis
in vitro digestion
fish lipids
bile salts
title_short A study by 1H NMR on the influence of some factors affecting lipid in vitro digestion
title_full A study by 1H NMR on the influence of some factors affecting lipid in vitro digestion
title_fullStr A study by 1H NMR on the influence of some factors affecting lipid in vitro digestion
title_full_unstemmed A study by 1H NMR on the influence of some factors affecting lipid in vitro digestion
title_sort A study by 1H NMR on the influence of some factors affecting lipid in vitro digestion
dc.creator.none.fl_str_mv Nieva Echevarría, Bárbara
Goikoetxea Osés, Encarnación
Manzanos Arnaiz, María José
Guillén Loren, María Dolores
author Nieva Echevarría, Bárbara
author_facet Nieva Echevarría, Bárbara
Goikoetxea Osés, Encarnación
Manzanos Arnaiz, María José
Guillén Loren, María Dolores
author_role author
author2 Goikoetxea Osés, Encarnación
Manzanos Arnaiz, María José
Guillén Loren, María Dolores
author2_role author
author
author
dc.subject.none.fl_str_mv 1H NMR
lipolysis
in vitro digestion
fish lipids
bile salts
topic 1H NMR
lipolysis
in vitro digestion
fish lipids
bile salts
description This article focuses on the impact of several experimental factors, including gastric acidification, intestinal transit time, presence of gastric lipase, sample/digestive fluids ratio, concentration and nature of the enzymes in intestinal juice, and bile concentration, on the extent of in vitro lipolysis when using a static model that simulates human digestion processes in mouth, stomach and small intestine. The study was carried out by Proton Nuclear Magnetic Resonance (1H NMR). This technique provides a complete molecular picture of lipolysis, evidencing for the first time, whether preferential hydrolysis of certain glycerides over others occurs. A lipolysis degree similar to that reported in vivo was reached by varying certain variables within a physiological range; among them, bile concentration was found to be crucial. The holistic view of this 1H NMR study provides information of paramount importance to design sound in vitro digestion models to determine the bioaccessibility and bioavailability of lipophilic compounds.
publishDate 2016
dc.date.none.fl_str_mv 2016
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/64628
url http://hdl.handle.net/10810/64628
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MINECO/AGL2012-36466/
info:eu-repo/grantAgreement/MINECO/AGL2015-65450-R/
https://doi.org/10.1016/j.foodchem.2016.05.021
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
Atribución-NoComercial-SinDerivadas 3.0 España
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/3.0/es/
Atribución-NoComercial-SinDerivadas 3.0 España
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevierj
publisher.none.fl_str_mv Elsevierj
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
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repository.mail.fl_str_mv
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