Microbiome Composition by Pyrosequencing in Mesenteric Lymph Nodes of Rats with CCI4-induced Cirrhosis

Background: The cross talk between the gut microbiota and the immune system, which is essential to maintain homeostasis, takes place at the intestinal lymphoid tissue such as the mesenteric lymph nodes (MLNs). Here, we investigated the presence of bacterial DNA in MLNs of control and cirrhotic rats...

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Autores: Cuenca, S, Sanchez, E, Santiago, A, El Khader, I, Panda, S, Vidal, S, Nieto, JC, Juarez, C, Sancho, F, Guarner, F, Soriano, G, Guarner, C, Manichanh, C
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Institución:Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)
Repositorio:r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
OAI Identifier:oai:iibsantpau.fundanetsuite.com:p9434
Acceso en línea:https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=9434
Access Level:acceso abierto
Palabra clave:Carbon tetrachloride
Cirrhosis
Microbiota
Mesenteric lymph node
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spelling Microbiome Composition by Pyrosequencing in Mesenteric Lymph Nodes of Rats with CCI4-induced CirrhosisCuenca, SSanchez, ESantiago, AEl Khader, IPanda, SVidal, SNieto, JCJuarez, CSancho, FGuarner, FSoriano, GGuarner, CManichanh, CCarbon tetrachlorideCirrhosisMicrobiotaMesenteric lymph nodeBackground: The cross talk between the gut microbiota and the immune system, which is essential to maintain homeostasis, takes place at the intestinal lymphoid tissue such as the mesenteric lymph nodes (MLNs). Here, we investigated the presence of bacterial DNA in MLNs of control and cirrhotic rats and its relationship with inflammatory responses. Methods:The MLN microbiome of cirrhotic rats with ascites, which was induced by carbon tetrachloride (CCI4), was compared to that of control rats using quantitative real-time PCR and pyrosequencing of the 16S rRNA gene. Cytokines in blood samples were assessed by ELISA. Results: Unexpectedly, sequence analysis revealed a high microbial diversity in the MLNs of both control and cirrhotic rats with Proteobacteria as one of the most dominant phylum. CCI4-induced liver injury was not associated with a change in bacterial load, but it was linked to a decrease in microbial diversity (p<0.05) and alterations in the microbial community in MLNs. A high proportion of Bifidobacterium animalis was also positively correlated with elevated interleukin-10 expression (p = 0.002, false discovery rate = 0.03, r = 0.94). Conclusions: For the first time, the high microbial diversity observed in MLNs of both controls and CCI4-induced cirrhotic rats provides evidence that bacterial translocation is more than a mere dichotomic phenomenon. (C) 2013 S. Karger AG, BaselKARGER2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=9434Journal of Innate ImmunityISSN: 1662811XISSNe: 16628128reponame:r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pauinstname:Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)Inglésinfo:eu-repo/semantics/openAccessoai:iibsantpau.fundanetsuite.com:p94342026-06-14T12:41:47Z
dc.title.none.fl_str_mv Microbiome Composition by Pyrosequencing in Mesenteric Lymph Nodes of Rats with CCI4-induced Cirrhosis
title Microbiome Composition by Pyrosequencing in Mesenteric Lymph Nodes of Rats with CCI4-induced Cirrhosis
spellingShingle Microbiome Composition by Pyrosequencing in Mesenteric Lymph Nodes of Rats with CCI4-induced Cirrhosis
Cuenca, S
Carbon tetrachloride
Cirrhosis
Microbiota
Mesenteric lymph node
title_short Microbiome Composition by Pyrosequencing in Mesenteric Lymph Nodes of Rats with CCI4-induced Cirrhosis
title_full Microbiome Composition by Pyrosequencing in Mesenteric Lymph Nodes of Rats with CCI4-induced Cirrhosis
title_fullStr Microbiome Composition by Pyrosequencing in Mesenteric Lymph Nodes of Rats with CCI4-induced Cirrhosis
title_full_unstemmed Microbiome Composition by Pyrosequencing in Mesenteric Lymph Nodes of Rats with CCI4-induced Cirrhosis
title_sort Microbiome Composition by Pyrosequencing in Mesenteric Lymph Nodes of Rats with CCI4-induced Cirrhosis
dc.creator.none.fl_str_mv Cuenca, S
Sanchez, E
Santiago, A
El Khader, I
Panda, S
Vidal, S
Nieto, JC
Juarez, C
Sancho, F
Guarner, F
Soriano, G
Guarner, C
Manichanh, C
author Cuenca, S
author_facet Cuenca, S
Sanchez, E
Santiago, A
El Khader, I
Panda, S
Vidal, S
Nieto, JC
Juarez, C
Sancho, F
Guarner, F
Soriano, G
Guarner, C
Manichanh, C
author_role author
author2 Sanchez, E
Santiago, A
El Khader, I
Panda, S
Vidal, S
Nieto, JC
Juarez, C
Sancho, F
Guarner, F
Soriano, G
Guarner, C
Manichanh, C
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Carbon tetrachloride
Cirrhosis
Microbiota
Mesenteric lymph node
topic Carbon tetrachloride
Cirrhosis
Microbiota
Mesenteric lymph node
description Background: The cross talk between the gut microbiota and the immune system, which is essential to maintain homeostasis, takes place at the intestinal lymphoid tissue such as the mesenteric lymph nodes (MLNs). Here, we investigated the presence of bacterial DNA in MLNs of control and cirrhotic rats and its relationship with inflammatory responses. Methods:The MLN microbiome of cirrhotic rats with ascites, which was induced by carbon tetrachloride (CCI4), was compared to that of control rats using quantitative real-time PCR and pyrosequencing of the 16S rRNA gene. Cytokines in blood samples were assessed by ELISA. Results: Unexpectedly, sequence analysis revealed a high microbial diversity in the MLNs of both control and cirrhotic rats with Proteobacteria as one of the most dominant phylum. CCI4-induced liver injury was not associated with a change in bacterial load, but it was linked to a decrease in microbial diversity (p<0.05) and alterations in the microbial community in MLNs. A high proportion of Bifidobacterium animalis was also positively correlated with elevated interleukin-10 expression (p = 0.002, false discovery rate = 0.03, r = 0.94). Conclusions: For the first time, the high microbial diversity observed in MLNs of both controls and CCI4-induced cirrhotic rats provides evidence that bacterial translocation is more than a mere dichotomic phenomenon. (C) 2013 S. Karger AG, Basel
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=9434
url https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=9434
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv KARGER
publisher.none.fl_str_mv KARGER
dc.source.none.fl_str_mv Journal of Innate Immunity
ISSN: 1662811X
ISSNe: 16628128
reponame:r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
instname:Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)
instname_str Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)
reponame_str r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
collection r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
repository.name.fl_str_mv
repository.mail.fl_str_mv
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