Comparative cytogenetic study of three Macrolophus species (Heteroptera, Miridae.)

Macrolophus pygmaeus (Rambur, 1839) (Insecta, Heteroptera, Miridae) is a predator of key vegetable crop pests applied as a biocontrol agent in the Mediterranean region. M. pygmaeus and M. melanotoma (A. Costa, 1853) are cryptic species with great morphological similarity which results in their misid...

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Autores: Jauset Berrocal, Ana Maria, Edo-Tena, Eva, Castañé Fernández, Cristina, Agustí Abella, Núria, Alomar, Òscar, Grozeva, S.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Institución:Universitat de Lleida (UdL)
Repositorio:Repositori Obert UdL
OAI Identifier:oai:repositori.udl.cat:10459.1/49194
Acceso en línea:https://doi.org/10.3897/CompCytogen.v9i4.5530
http://hdl.handle.net/10459.1/49194
Access Level:acceso abierto
Palabra clave:Macrolophus
Miridae
Karyotype
sperm morphology
achiasmate meiosis
Control biològic de plagues
Mediterrània (Regió)
Hemípters
Citogenètica
Biological pest control
Mediterranean Region
Hemiptera
Cytogenetics
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spelling Comparative cytogenetic study of three Macrolophus species (Heteroptera, Miridae.)Jauset Berrocal, Ana MariaEdo-Tena, EvaCastañé Fernández, CristinaAgustí Abella, NúriaAlomar, ÒscarGrozeva, S.MacrolophusMiridaeKaryotypesperm morphologyachiasmate meiosisControl biològic de plaguesMediterrània (Regió)HemíptersCitogenèticaBiological pest controlMediterranean RegionHemipteraCytogeneticsMacrolophus pygmaeus (Rambur, 1839) (Insecta, Heteroptera, Miridae) is a predator of key vegetable crop pests applied as a biocontrol agent in the Mediterranean region. M. pygmaeus and M. melanotoma (A. Costa, 1853) are cryptic species with great morphological similarity which results in their misidentification and negative consequences for the conservation of their populations on greenhouse and outdoor crops. In order to find out specific markers for their separation we studied the karyotype, male meiosis and heterochromatin composition of these species and additionally of a third species (as a reference one), M. costalis Fieber, 1858. We demonstrate here that all the three species share achiasmate male meiosis and sex chromosome pre-reduction. On the other hand, the species differ in karyotype, with 2n=28 (26+XY) in M. pygmaeus, 2n=27 (24+X1X2Y) in M. costalis, and 2n=34 (32+XY) in M. melanotoma, and heterochromatin distribution and composition. In addition, the species differ in sperm morphology: sperm cells of M. costalis are significantly longer with longer head and tail than those of M. melanotoma and M. pygmaeus, whereas sperm cells of M. melanotoma have a longer tail than those of M. pygmaeus. All these characters can be used as markers to identify the species, in particular the cryptic species M. melanotoma and M. pygmaeus.This study has been funded by the Spanish Ministry of Economy and Competitiveness (MINECO) (Project AGL2011-24349), and by a travel grant from the University of Lleida - Fundació “La Caixa” to Dr Grozeva. The chromosome analysis was performed using microscope Axio Scope A1 – Carl Zeiss Microscopy upgraded by the project WETLANET (FP7 CSA – SUPPORT ACTION, GA 229802). We thank cordially Prof. Dr V. Kuznetsova for the valuable advices to improve the manuscriptPensoft Publishers2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://doi.org/10.3897/CompCytogen.v9i4.5530http://hdl.handle.net/10459.1/49194reponame:Repositori Obert UdL instname:Universitat de Lleida (UdL)Inglésinfo:eu-repo/grantAgreement/MICINN//AGL2011-24349Reproducció del document publicat a: https://doi.org/10.3897/CompCytogen.v9i4.5530Comparative Cytogenetics, 2015, vol. 9, núm. 4, p. 613-623info:eu-repo/grantAgreement/EC/FP7/229802cc-by (c) Jauset et al., 2015info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/oai:repositori.udl.cat:10459.1/491942026-06-24T12:42:17Z
dc.title.none.fl_str_mv Comparative cytogenetic study of three Macrolophus species (Heteroptera, Miridae.)
title Comparative cytogenetic study of three Macrolophus species (Heteroptera, Miridae.)
spellingShingle Comparative cytogenetic study of three Macrolophus species (Heteroptera, Miridae.)
Jauset Berrocal, Ana Maria
Macrolophus
Miridae
Karyotype
sperm morphology
achiasmate meiosis
Control biològic de plagues
Mediterrània (Regió)
Hemípters
Citogenètica
Biological pest control
Mediterranean Region
Hemiptera
Cytogenetics
title_short Comparative cytogenetic study of three Macrolophus species (Heteroptera, Miridae.)
title_full Comparative cytogenetic study of three Macrolophus species (Heteroptera, Miridae.)
title_fullStr Comparative cytogenetic study of three Macrolophus species (Heteroptera, Miridae.)
title_full_unstemmed Comparative cytogenetic study of three Macrolophus species (Heteroptera, Miridae.)
title_sort Comparative cytogenetic study of three Macrolophus species (Heteroptera, Miridae.)
dc.creator.none.fl_str_mv Jauset Berrocal, Ana Maria
Edo-Tena, Eva
Castañé Fernández, Cristina
Agustí Abella, Núria
Alomar, Òscar
Grozeva, S.
author Jauset Berrocal, Ana Maria
author_facet Jauset Berrocal, Ana Maria
Edo-Tena, Eva
Castañé Fernández, Cristina
Agustí Abella, Núria
Alomar, Òscar
Grozeva, S.
author_role author
author2 Edo-Tena, Eva
Castañé Fernández, Cristina
Agustí Abella, Núria
Alomar, Òscar
Grozeva, S.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Macrolophus
Miridae
Karyotype
sperm morphology
achiasmate meiosis
Control biològic de plagues
Mediterrània (Regió)
Hemípters
Citogenètica
Biological pest control
Mediterranean Region
Hemiptera
Cytogenetics
topic Macrolophus
Miridae
Karyotype
sperm morphology
achiasmate meiosis
Control biològic de plagues
Mediterrània (Regió)
Hemípters
Citogenètica
Biological pest control
Mediterranean Region
Hemiptera
Cytogenetics
description Macrolophus pygmaeus (Rambur, 1839) (Insecta, Heteroptera, Miridae) is a predator of key vegetable crop pests applied as a biocontrol agent in the Mediterranean region. M. pygmaeus and M. melanotoma (A. Costa, 1853) are cryptic species with great morphological similarity which results in their misidentification and negative consequences for the conservation of their populations on greenhouse and outdoor crops. In order to find out specific markers for their separation we studied the karyotype, male meiosis and heterochromatin composition of these species and additionally of a third species (as a reference one), M. costalis Fieber, 1858. We demonstrate here that all the three species share achiasmate male meiosis and sex chromosome pre-reduction. On the other hand, the species differ in karyotype, with 2n=28 (26+XY) in M. pygmaeus, 2n=27 (24+X1X2Y) in M. costalis, and 2n=34 (32+XY) in M. melanotoma, and heterochromatin distribution and composition. In addition, the species differ in sperm morphology: sperm cells of M. costalis are significantly longer with longer head and tail than those of M. melanotoma and M. pygmaeus, whereas sperm cells of M. melanotoma have a longer tail than those of M. pygmaeus. All these characters can be used as markers to identify the species, in particular the cryptic species M. melanotoma and M. pygmaeus.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.3897/CompCytogen.v9i4.5530
http://hdl.handle.net/10459.1/49194
url https://doi.org/10.3897/CompCytogen.v9i4.5530
http://hdl.handle.net/10459.1/49194
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MICINN//AGL2011-24349
Reproducció del document publicat a: https://doi.org/10.3897/CompCytogen.v9i4.5530
Comparative Cytogenetics, 2015, vol. 9, núm. 4, p. 613-623
info:eu-repo/grantAgreement/EC/FP7/229802
dc.rights.none.fl_str_mv cc-by (c) Jauset et al., 2015
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv cc-by (c) Jauset et al., 2015
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pensoft Publishers
publisher.none.fl_str_mv Pensoft Publishers
dc.source.none.fl_str_mv reponame:Repositori Obert UdL
instname:Universitat de Lleida (UdL)
instname_str Universitat de Lleida (UdL)
reponame_str Repositori Obert UdL
collection Repositori Obert UdL
repository.name.fl_str_mv
repository.mail.fl_str_mv
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