Herpes simplex virus type 1 induces AD-like neurodegeneration markers in human progenitor and differentiated ReNcell VM cells

An increasing body of evidence strongly suggests that infections or reactivations of herpes simplex virus type 1 (HSV-1) may be closely linked to Alzheimer’s disease (AD). Promising results have been obtained using cell and animal models of HSV-1 infection, contributing to the understanding of the m...

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Autores: Salgado Fuentes, Blanca, Sastre, Isabel, Bullido Gómez-Heras, María Jesús, Aldudo Soto, Jesús
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/707939
Acceso en línea:http://hdl.handle.net/10486/707939
https://dx.doi.org/10.3390/microorganisms11051205
Access Level:acceso abierto
Palabra clave:Human Alphaherpesvirus 1
Infection
Fungus Antibody
Biología y Biomedicina / Biología
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spelling Herpes simplex virus type 1 induces AD-like neurodegeneration markers in human progenitor and differentiated ReNcell VM cellsSalgado Fuentes, BlancaSastre, IsabelBullido Gómez-Heras, María JesúsAldudo Soto, JesúsHuman Alphaherpesvirus 1InfectionFungus AntibodyBiología y Biomedicina / BiologíaAn increasing body of evidence strongly suggests that infections or reactivations of herpes simplex virus type 1 (HSV-1) may be closely linked to Alzheimer’s disease (AD). Promising results have been obtained using cell and animal models of HSV-1 infection, contributing to the understanding of the molecular mechanisms linking HSV-1 infection and AD neurodegeneration. ReNcell VM is a human neural stem cell line that has been used as a model system to study the impact of various infectious agents on the central nervous system. In this study, we demonstrate the suitability of the ReNcell VM cell line for developing a new in vitro model of HSV-1 infection. By following standard differentiation protocols, we were able to derive various nervous cell types, including neurons, astrocytes, and oligodendrocytes, from neural precursors. Additionally, we demonstrated the susceptibility of ReNcell VM cells, including precursor and differentiated cells, to HSV-1 infection and subsequent viral-induced AD-like neurodegeneration. Our findings support the use of this cell line to generate a new research platform for investigating AD neuropathology and its most significant risk factors, which may lead to important discoveries in the context of this highly impactful diseaseThis work was funded by the Spanish Ministerio de Ciencia e Innovación (PID2020-113921RBI00)MDPIDepartamento de Biología MolecularFacultad de Ciencias20232023-05-01research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/707939https://dx.doi.org/10.3390/microorganisms11051205reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/7079392026-06-23T12:46:27Z
dc.title.none.fl_str_mv Herpes simplex virus type 1 induces AD-like neurodegeneration markers in human progenitor and differentiated ReNcell VM cells
title Herpes simplex virus type 1 induces AD-like neurodegeneration markers in human progenitor and differentiated ReNcell VM cells
spellingShingle Herpes simplex virus type 1 induces AD-like neurodegeneration markers in human progenitor and differentiated ReNcell VM cells
Salgado Fuentes, Blanca
Human Alphaherpesvirus 1
Infection
Fungus Antibody
Biología y Biomedicina / Biología
title_short Herpes simplex virus type 1 induces AD-like neurodegeneration markers in human progenitor and differentiated ReNcell VM cells
title_full Herpes simplex virus type 1 induces AD-like neurodegeneration markers in human progenitor and differentiated ReNcell VM cells
title_fullStr Herpes simplex virus type 1 induces AD-like neurodegeneration markers in human progenitor and differentiated ReNcell VM cells
title_full_unstemmed Herpes simplex virus type 1 induces AD-like neurodegeneration markers in human progenitor and differentiated ReNcell VM cells
title_sort Herpes simplex virus type 1 induces AD-like neurodegeneration markers in human progenitor and differentiated ReNcell VM cells
dc.creator.none.fl_str_mv Salgado Fuentes, Blanca
Sastre, Isabel
Bullido Gómez-Heras, María Jesús
Aldudo Soto, Jesús
author Salgado Fuentes, Blanca
author_facet Salgado Fuentes, Blanca
Sastre, Isabel
Bullido Gómez-Heras, María Jesús
Aldudo Soto, Jesús
author_role author
author2 Sastre, Isabel
Bullido Gómez-Heras, María Jesús
Aldudo Soto, Jesús
author2_role author
author
author
dc.contributor.none.fl_str_mv Departamento de Biología Molecular
Facultad de Ciencias
dc.subject.none.fl_str_mv Human Alphaherpesvirus 1
Infection
Fungus Antibody
Biología y Biomedicina / Biología
topic Human Alphaherpesvirus 1
Infection
Fungus Antibody
Biología y Biomedicina / Biología
description An increasing body of evidence strongly suggests that infections or reactivations of herpes simplex virus type 1 (HSV-1) may be closely linked to Alzheimer’s disease (AD). Promising results have been obtained using cell and animal models of HSV-1 infection, contributing to the understanding of the molecular mechanisms linking HSV-1 infection and AD neurodegeneration. ReNcell VM is a human neural stem cell line that has been used as a model system to study the impact of various infectious agents on the central nervous system. In this study, we demonstrate the suitability of the ReNcell VM cell line for developing a new in vitro model of HSV-1 infection. By following standard differentiation protocols, we were able to derive various nervous cell types, including neurons, astrocytes, and oligodendrocytes, from neural precursors. Additionally, we demonstrated the susceptibility of ReNcell VM cells, including precursor and differentiated cells, to HSV-1 infection and subsequent viral-induced AD-like neurodegeneration. Our findings support the use of this cell line to generate a new research platform for investigating AD neuropathology and its most significant risk factors, which may lead to important discoveries in the context of this highly impactful disease
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-05-01
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/707939
https://dx.doi.org/10.3390/microorganisms11051205
url http://hdl.handle.net/10486/707939
https://dx.doi.org/10.3390/microorganisms11051205
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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