Expression and molecular analysis of the Arabidopsis DXR gene encoding 1-Deoxy-D-Xylulose 5-phosphate reductoisomerase, the first commited enzyme of the 2-C-methyl-D-erythritol 4-phosphate pathway.

Carretero Paulet, Lorenzo; Ahumada, Iván; Cunillera i Segarra, Núria; Rodríguez Concepción, Manuel; Ferrer i Prats, Albert; Boronat i Margosa, Albert; Campos Martínez, Narciso

Expression and molecular analysis of the Arabidopsis DXR gene encoding 1-Deoxy-D-Xylulose 5-phosphate reductoisomerase, the first commited enzyme of the 2-C-methyl-D-erythritol 4-phosphate pathway.

1-Deoxy-d-xylulose 5-phosphate reductoisomerase (DXR) catalyzes the first committed step of the 2-C-methyl-d-erythritol 4-phosphate pathway for isoprenoid biosynthesis. In Arabidopsis, DXR is encoded by a single-copy gene. We have cloned a full-length cDNA corresponding to this gene. A comparative a...

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Authors:	Carretero Paulet, Lorenzo, Ahumada, Iván, Cunillera i Segarra, Núria, Rodríguez Concepción, Manuel, Ferrer i Prats, Albert, Boronat i Margosa, Albert, Campos Martínez, Narciso
Format:	article
Status:	Versión aceptada para publicación
Publication Date:	2002
Country:	España
Institution:	Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repository:	Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:	oai:recercat.cat:2445/225893
Online Access:	https://hdl.handle.net/2445/225893
Access Level:	Open access
Keyword:	Biologia molecular vegetal Genètica molecular vegetal Plant molecular biology Plant molecular genetics

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spelling	Expression and molecular analysis of the Arabidopsis DXR gene encoding 1-Deoxy-D-Xylulose 5-phosphate reductoisomerase, the first commited enzyme of the 2-C-methyl-D-erythritol 4-phosphate pathway.Carretero Paulet, LorenzoAhumada, IvánCunillera i Segarra, NúriaRodríguez Concepción, ManuelFerrer i Prats, AlbertBoronat i Margosa, AlbertCampos Martínez, NarcisoBiologia molecular vegetalGenètica molecular vegetalPlant molecular biologyPlant molecular genetics1-Deoxy-d-xylulose 5-phosphate reductoisomerase (DXR) catalyzes the first committed step of the 2-C-methyl-d-erythritol 4-phosphate pathway for isoprenoid biosynthesis. In Arabidopsis, DXR is encoded by a single-copy gene. We have cloned a full-length cDNA corresponding to this gene. A comparative analysis of all plant DXR sequences known to date predicted an N-terminal transit peptide for plastids, with a conserved cleavage site, and a conserved proline-rich region at the N terminus of the mature protein, which is not present in the prokaryotic DXR homologs. We demonstrate that Arabidopsis DXR is targeted to plastids and localizes into chloroplasts of leaf cells. The presence of the proline-rich region in the mature Arabidopsis DXR was confirmed by detection with a specific antibody. A proof of the enzymatic function of this protein was obtained by complementation of anEscherichia coli mutant defective in DXR activity. The expression pattern of β-glucuronidase, driven by theDXR promoter in Arabidopsis transgenic plants, together with the tissue distribution of DXR transcript and protein, revealed developmental and environmental regulation of theDXR gene. The expression pattern of theDXR gene parallels that of the Arabidopsis 1-deoxy-d-xylulose 5-phosphate synthase gene, but the former is slightly more restricted. These genes are expressed in most organs of the plant including roots, with higher levels in seedlings and inflorescences. The block of the 2-C-methyl-d-erythritol 4-phosphate pathway in Arabidopsis seedlings with fosmidomycin led to a rapid accumulation of DXR protein, whereas the 1-deoxy-d-xylulose 5-phosphate synthase protein level was not altered. Our results are consistent with the participation of the Arabidopsis DXR gene in the control of the 2-C-methyl-d-erythritol 4-phosphate pathway.American Society of Plant Biologists2026202620022026info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion11 p.application/pdfhttps://hdl.handle.net/2445/225893Articles publicats en revistes (Bioquímica i Fisiologia)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: https://doi.org/10.1104/pp.003798Plant Physiology, 2002, vol. 129, num.4, p. 1581-1591https://doi.org/10.1104/pp.003798(c) American Society of Plant Biologists, 2002info:eu-repo/semantics/openAccessoai:recercat.cat:2445/2258932026-05-29T05:05:01Z
dc.title.none.fl_str_mv	Expression and molecular analysis of the Arabidopsis DXR gene encoding 1-Deoxy-D-Xylulose 5-phosphate reductoisomerase, the first commited enzyme of the 2-C-methyl-D-erythritol 4-phosphate pathway.
title	Expression and molecular analysis of the Arabidopsis DXR gene encoding 1-Deoxy-D-Xylulose 5-phosphate reductoisomerase, the first commited enzyme of the 2-C-methyl-D-erythritol 4-phosphate pathway.
spellingShingle	Expression and molecular analysis of the Arabidopsis DXR gene encoding 1-Deoxy-D-Xylulose 5-phosphate reductoisomerase, the first commited enzyme of the 2-C-methyl-D-erythritol 4-phosphate pathway. Carretero Paulet, Lorenzo Biologia molecular vegetal Genètica molecular vegetal Plant molecular biology Plant molecular genetics
title_short	Expression and molecular analysis of the Arabidopsis DXR gene encoding 1-Deoxy-D-Xylulose 5-phosphate reductoisomerase, the first commited enzyme of the 2-C-methyl-D-erythritol 4-phosphate pathway.
title_full	Expression and molecular analysis of the Arabidopsis DXR gene encoding 1-Deoxy-D-Xylulose 5-phosphate reductoisomerase, the first commited enzyme of the 2-C-methyl-D-erythritol 4-phosphate pathway.
title_fullStr	Expression and molecular analysis of the Arabidopsis DXR gene encoding 1-Deoxy-D-Xylulose 5-phosphate reductoisomerase, the first commited enzyme of the 2-C-methyl-D-erythritol 4-phosphate pathway.
title_full_unstemmed	Expression and molecular analysis of the Arabidopsis DXR gene encoding 1-Deoxy-D-Xylulose 5-phosphate reductoisomerase, the first commited enzyme of the 2-C-methyl-D-erythritol 4-phosphate pathway.
title_sort	Expression and molecular analysis of the Arabidopsis DXR gene encoding 1-Deoxy-D-Xylulose 5-phosphate reductoisomerase, the first commited enzyme of the 2-C-methyl-D-erythritol 4-phosphate pathway.
dc.creator.none.fl_str_mv	Carretero Paulet, Lorenzo Ahumada, Iván Cunillera i Segarra, Núria Rodríguez Concepción, Manuel Ferrer i Prats, Albert Boronat i Margosa, Albert Campos Martínez, Narciso
author	Carretero Paulet, Lorenzo
author_facet	Carretero Paulet, Lorenzo Ahumada, Iván Cunillera i Segarra, Núria Rodríguez Concepción, Manuel Ferrer i Prats, Albert Boronat i Margosa, Albert Campos Martínez, Narciso
author_role	author
author2	Ahumada, Iván Cunillera i Segarra, Núria Rodríguez Concepción, Manuel Ferrer i Prats, Albert Boronat i Margosa, Albert Campos Martínez, Narciso
author2_role	author author author author author author
dc.subject.none.fl_str_mv	Biologia molecular vegetal Genètica molecular vegetal Plant molecular biology Plant molecular genetics
topic	Biologia molecular vegetal Genètica molecular vegetal Plant molecular biology Plant molecular genetics
description	1-Deoxy-d-xylulose 5-phosphate reductoisomerase (DXR) catalyzes the first committed step of the 2-C-methyl-d-erythritol 4-phosphate pathway for isoprenoid biosynthesis. In Arabidopsis, DXR is encoded by a single-copy gene. We have cloned a full-length cDNA corresponding to this gene. A comparative analysis of all plant DXR sequences known to date predicted an N-terminal transit peptide for plastids, with a conserved cleavage site, and a conserved proline-rich region at the N terminus of the mature protein, which is not present in the prokaryotic DXR homologs. We demonstrate that Arabidopsis DXR is targeted to plastids and localizes into chloroplasts of leaf cells. The presence of the proline-rich region in the mature Arabidopsis DXR was confirmed by detection with a specific antibody. A proof of the enzymatic function of this protein was obtained by complementation of anEscherichia coli mutant defective in DXR activity. The expression pattern of β-glucuronidase, driven by theDXR promoter in Arabidopsis transgenic plants, together with the tissue distribution of DXR transcript and protein, revealed developmental and environmental regulation of theDXR gene. The expression pattern of theDXR gene parallels that of the Arabidopsis 1-deoxy-d-xylulose 5-phosphate synthase gene, but the former is slightly more restricted. These genes are expressed in most organs of the plant including roots, with higher levels in seedlings and inflorescences. The block of the 2-C-methyl-d-erythritol 4-phosphate pathway in Arabidopsis seedlings with fosmidomycin led to a rapid accumulation of DXR protein, whereas the 1-deoxy-d-xylulose 5-phosphate synthase protein level was not altered. Our results are consistent with the participation of the Arabidopsis DXR gene in the control of the 2-C-methyl-d-erythritol 4-phosphate pathway.
publishDate	2002
dc.date.none.fl_str_mv	2002 2026 2026 2026
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion
format	article
status_str	acceptedVersion
dc.identifier.none.fl_str_mv	https://hdl.handle.net/2445/225893
url	https://hdl.handle.net/2445/225893
dc.language.none.fl_str_mv	Inglés
language_invalid_str_mv	Inglés
dc.relation.none.fl_str_mv	Versió postprint del document publicat a: https://doi.org/10.1104/pp.003798 Plant Physiology, 2002, vol. 129, num.4, p. 1581-1591 https://doi.org/10.1104/pp.003798
dc.rights.none.fl_str_mv	(c) American Society of Plant Biologists, 2002 info:eu-repo/semantics/openAccess
rights_invalid_str_mv	(c) American Society of Plant Biologists, 2002
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	11 p. application/pdf
dc.publisher.none.fl_str_mv	American Society of Plant Biologists
publisher.none.fl_str_mv	American Society of Plant Biologists
dc.source.none.fl_str_mv	Articles publicats en revistes (Bioquímica i Fisiologia) reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str	Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str	Recercat. Dipósit de la Recerca de Catalunya
collection	Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score	15,811543

Expression and molecular analysis of the Arabidopsis DXR gene encoding 1-Deoxy-D-Xylulose 5-phosphate reductoisomerase, the first commited enzyme of the 2-C-methyl-D-erythritol 4-phosphate pathway.

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