Biohybrids for spinal cord injury repair

[EN] Spinal cord injuries (SCIs) result in the loss of sensory and motor function with massive cell death and axon degeneration. We have previously shown that transplantation of spinal cord-derived ependymal progenitor cells (epSPC) significantly improves functional recovery after acute and chronic...

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Autores: Martínez-Ramos, Cristina|||0000-0002-6540-4714, Giraldo, Esther|||0000-0001-5488-3011, Monleón Pradas, Manuel|||0000-0001-6457-0414, Rodriguez Doblado, Laura, López Mocholi, Eric, Alastrue-Agudo, Ana, Sánchez Petidier, Marina, Moreno-Manzano, Victoria
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/160297
Acceso en línea:https://riunet.upv.es/handle/10251/160297
Access Level:acceso abierto
Palabra clave:Biomaterial
Hyaluronic acid
Neural differentiation
Poly-lactic fibres
Spinal cord injury
TERMODINAMICA APLICADA (UPV)
MICROBIOLOGIA
MAQUINAS Y MOTORES TERMICOS
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spelling Biohybrids for spinal cord injury repairMartínez-Ramos, Cristina|||0000-0002-6540-4714Giraldo, Esther|||0000-0001-5488-3011Monleón Pradas, Manuel|||0000-0001-6457-0414Rodriguez Doblado, LauraLópez Mocholi, EricAlastrue-Agudo, AnaSánchez Petidier, MarinaMoreno-Manzano, VictoriaBiomaterialHyaluronic acidNeural differentiationPoly-lactic fibresSpinal cord injuryTERMODINAMICA APLICADA (UPV)MICROBIOLOGIAMAQUINAS Y MOTORES TERMICOS[EN] Spinal cord injuries (SCIs) result in the loss of sensory and motor function with massive cell death and axon degeneration. We have previously shown that transplantation of spinal cord-derived ependymal progenitor cells (epSPC) significantly improves functional recovery after acute and chronic SCI in experimental models, via neuronal differentiation and trophic glial cell support. Here, we propose an improved procedure based on transplantation of epSPC in a tubular conduit of hyaluronic acid containing poly (lactic acid) fibres creating a biohybrid scaffold. In vitro analysis showed that the poly (lactic acid) fibres included in the conduit induce a preferential neuronal fate of the epSPC rather than glial differentiation, favouring elongation of cellular processes. The safety and efficacy of the biohybrid implantation was evaluated in a complete SCI rat model. The conduits allowed efficient epSPC transfer into the spinal cord, improving the preservation of the neuronal tissue by increasing the presence of neuronal fibres at the injury site and by reducing cavities and cyst formation. The biohybrid-implanted animals presented diminished astrocytic reactivity surrounding the scar area, an increased number of preserved neuronal fibres with a horizontal directional pattern, and enhanced coexpression of the growth cone marker GAP43. The biohybrids offer an improved method for cell transplantation with potential capabilities for neuronal tissue regeneration, opening a promising avenue for cell therapies and SCI treatment.Secretaria de Estado de Investigacion, Desarrollo e Innovacion, Grant/Award Number: MAT2015-66666-C3-1-R MINECO/FEDER MAT2015-66666-C3-2-R MINECO/FEDER; Spanish Ministry of Education, Culture and Sports through Laura Rodriguez Doblado, Grant/Award Number: FPU15/04975John Wiley & SonsDepartamento de BiotecnologíaDepartamento de Termodinámica AplicadaCentro de Biomateriales e Ingeniería TisularEscuela Técnica Superior de Ingeniería IndustrialCentro Avanzado de Microbiología AplicadaEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalEuropean Regional Development FundMinisterio de Economía y CompetitividadMinisterio de Educación, Cultura y DeporteRepositorio Institucional de la Universitat Politècnica de València Riunet20192019-03-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://riunet.upv.es/handle/10251/160297reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 MAT2015-66666-C3-2-R BIOHIBRIDOS PARA LA PROMOCION DEL CRECIMIENTO AXONAL Y LA REGENERACION EN LESION MEDULAR AGUDA Y CRONICAMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 MAT2015-66666-C3-1-R BIOHIBRIDOS PARA LA PROMOCION DEL CRECIMIENTO AXONAL Y LA REGENERACION EN EL SISTEMA NERVIOSO CENTRAL Y PERIFERICOMinisterio de Educación y Cultura http://dx.doi.org/10.13039/501100003176 FPU15%2F04975 FPU15%2F04975open accesshttp://purl.org/coar/access_right/c_abf2Reserva de todos los derechoshttp://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/1602972026-06-13T07:49:27Z
dc.title.none.fl_str_mv Biohybrids for spinal cord injury repair
title Biohybrids for spinal cord injury repair
spellingShingle Biohybrids for spinal cord injury repair
Martínez-Ramos, Cristina|||0000-0002-6540-4714
Biomaterial
Hyaluronic acid
Neural differentiation
Poly-lactic fibres
Spinal cord injury
TERMODINAMICA APLICADA (UPV)
MICROBIOLOGIA
MAQUINAS Y MOTORES TERMICOS
title_short Biohybrids for spinal cord injury repair
title_full Biohybrids for spinal cord injury repair
title_fullStr Biohybrids for spinal cord injury repair
title_full_unstemmed Biohybrids for spinal cord injury repair
title_sort Biohybrids for spinal cord injury repair
dc.creator.none.fl_str_mv Martínez-Ramos, Cristina|||0000-0002-6540-4714
Giraldo, Esther|||0000-0001-5488-3011
Monleón Pradas, Manuel|||0000-0001-6457-0414
Rodriguez Doblado, Laura
López Mocholi, Eric
Alastrue-Agudo, Ana
Sánchez Petidier, Marina
Moreno-Manzano, Victoria
author Martínez-Ramos, Cristina|||0000-0002-6540-4714
author_facet Martínez-Ramos, Cristina|||0000-0002-6540-4714
Giraldo, Esther|||0000-0001-5488-3011
Monleón Pradas, Manuel|||0000-0001-6457-0414
Rodriguez Doblado, Laura
López Mocholi, Eric
Alastrue-Agudo, Ana
Sánchez Petidier, Marina
Moreno-Manzano, Victoria
author_role author
author2 Giraldo, Esther|||0000-0001-5488-3011
Monleón Pradas, Manuel|||0000-0001-6457-0414
Rodriguez Doblado, Laura
López Mocholi, Eric
Alastrue-Agudo, Ana
Sánchez Petidier, Marina
Moreno-Manzano, Victoria
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Biotecnología
Departamento de Termodinámica Aplicada
Centro de Biomateriales e Ingeniería Tisular
Escuela Técnica Superior de Ingeniería Industrial
Centro Avanzado de Microbiología Aplicada
Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural
European Regional Development Fund
Ministerio de Economía y Competitividad
Ministerio de Educación, Cultura y Deporte
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Biomaterial
Hyaluronic acid
Neural differentiation
Poly-lactic fibres
Spinal cord injury
TERMODINAMICA APLICADA (UPV)
MICROBIOLOGIA
MAQUINAS Y MOTORES TERMICOS
topic Biomaterial
Hyaluronic acid
Neural differentiation
Poly-lactic fibres
Spinal cord injury
TERMODINAMICA APLICADA (UPV)
MICROBIOLOGIA
MAQUINAS Y MOTORES TERMICOS
description [EN] Spinal cord injuries (SCIs) result in the loss of sensory and motor function with massive cell death and axon degeneration. We have previously shown that transplantation of spinal cord-derived ependymal progenitor cells (epSPC) significantly improves functional recovery after acute and chronic SCI in experimental models, via neuronal differentiation and trophic glial cell support. Here, we propose an improved procedure based on transplantation of epSPC in a tubular conduit of hyaluronic acid containing poly (lactic acid) fibres creating a biohybrid scaffold. In vitro analysis showed that the poly (lactic acid) fibres included in the conduit induce a preferential neuronal fate of the epSPC rather than glial differentiation, favouring elongation of cellular processes. The safety and efficacy of the biohybrid implantation was evaluated in a complete SCI rat model. The conduits allowed efficient epSPC transfer into the spinal cord, improving the preservation of the neuronal tissue by increasing the presence of neuronal fibres at the injury site and by reducing cavities and cyst formation. The biohybrid-implanted animals presented diminished astrocytic reactivity surrounding the scar area, an increased number of preserved neuronal fibres with a horizontal directional pattern, and enhanced coexpression of the growth cone marker GAP43. The biohybrids offer an improved method for cell transplantation with potential capabilities for neuronal tissue regeneration, opening a promising avenue for cell therapies and SCI treatment.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-03-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/160297
url https://riunet.upv.es/handle/10251/160297
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 MAT2015-66666-C3-2-R BIOHIBRIDOS PARA LA PROMOCION DEL CRECIMIENTO AXONAL Y LA REGENERACION EN LESION MEDULAR AGUDA Y CRONICA
Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 MAT2015-66666-C3-1-R BIOHIBRIDOS PARA LA PROMOCION DEL CRECIMIENTO AXONAL Y LA REGENERACION EN EL SISTEMA NERVIOSO CENTRAL Y PERIFERICO
Ministerio de Educación y Cultura http://dx.doi.org/10.13039/501100003176 FPU15%2F04975 FPU15%2F04975
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reserva de todos los derechos
http://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reserva de todos los derechos
http://rightsstatements.org/vocab/InC/1.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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