Short-term electrostimulation enhancing neural repair in vitro using large charge capacity nanostructured electrodes

Electrostimulation of the neural system in functional or repair therapies requires new materials that protect the living system from electric field (EF) effects at the interface. Intercalation materials offer an alternative to radical formation during stimulation. Furthermore, nanostructuring of the...

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Autores: Lichtenstein, Mathieu, Pérez Soler, Estela, Ballesteros, Luis Alberto, Suñol, Cristina, Casañ Pastor, Nieves
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2017
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/155964
Acceso en línea:http://hdl.handle.net/10261/155964
Access Level:acceso abierto
Palabra clave:Electrostimulation
Electrode materials
Charge capacity
In vitro neurite regeneration
Iridium oxide
Graphene
Conducting polymers
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spelling Short-term electrostimulation enhancing neural repair in vitro using large charge capacity nanostructured electrodesLichtenstein, MathieuPérez Soler, EstelaBallesteros, Luis AlbertoSuñol, CristinaCasañ Pastor, NievesElectrostimulationElectrode materialsCharge capacityIn vitro neurite regenerationIridium oxideGrapheneConducting polymersElectrostimulation of the neural system in functional or repair therapies requires new materials that protect the living system from electric field (EF) effects at the interface. Intercalation materials offer an alternative to radical formation during stimulation. Furthermore, nanostructuring of the electroactive material used as electrode offers an enlargement of the charge capacity which in turn involves changes in the EF effect. In this work, electric field stimulation of cortical neuron cultures has been applied in an in vitro model of lesion, namely, a physical scratch in the cell culture creates a cell-free area reminiscent of a lesion where new neurites grow. Regeneration of the “wound” zone upon EF stimulation is observed for various types of electrode materials, and compared to the spontaneous process and to platinum electrodes. Significantly, electric field effects are highly dependent on the electrode material used, even for the same charge delivered and similar impedance values. Electrode coatings with large charge storage capacity yield significantly better results than that of bare Pt electrodes. Neurite outgrowth at the scratched “wound” zone is lowest, below spontaneous regeneration, when using Pt electrodes. On the other hand, electroactive materials, such as bilayers of PEDOT and polypyrrole with lysine counterions or iridium oxide-pristine graphene hybrids, promote further regeneration. Beyond impedance considerations, the optimal material is the nanostructured one with the largest charge capacity, even at low charge deliveries. It is remarkable that IrOx-graphene hybrids reach regenerations above spontaneous case in very short stimulation times, for equal charge deliveries and potential protocols. The implications from the results suggest that EF application using these new coatings, may have an immediate use in safer electrostimulation procedures, and open routes for much needed neural repair.This work was supported by grants from the Spanish MEC (MAT2011-24363 and MAT2015-65192-R), Marató TV3 Grant (110130/31), CSIC Ref. 201560E053 and Instituto de Salud Carlos III (PI 10/453), and Cerca programme Generalitat de Catalunya (2014/SGR/625, 2014/SGR/1643), and Severo Ochoa Programme for Centres of Excellence in R&D (SEV-2015-0496). L. Ballesteros thanks financing from CONICYT Fondecyt Grant No. 3150143. The technical assistance of Elisenda Marti is acknowledged.Peer reviewedElsevierMinisterio de Educación y Cultura (España)Ministerio de Economía y Competitividad (España)Fundació La Marató de TV3Consejo Superior de Investigaciones Científicas (España)Instituto de Salud Carlos IIIGeneralitat de CatalunyaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201720172017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/155964reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/MAT2015-65192-Rinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SEV-2015-0496http://dx.doi.org/10.1016/j.apmt.2016.12.002Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1559642026-05-22T06:33:51Z
dc.title.none.fl_str_mv Short-term electrostimulation enhancing neural repair in vitro using large charge capacity nanostructured electrodes
title Short-term electrostimulation enhancing neural repair in vitro using large charge capacity nanostructured electrodes
spellingShingle Short-term electrostimulation enhancing neural repair in vitro using large charge capacity nanostructured electrodes
Lichtenstein, Mathieu
Electrostimulation
Electrode materials
Charge capacity
In vitro neurite regeneration
Iridium oxide
Graphene
Conducting polymers
title_short Short-term electrostimulation enhancing neural repair in vitro using large charge capacity nanostructured electrodes
title_full Short-term electrostimulation enhancing neural repair in vitro using large charge capacity nanostructured electrodes
title_fullStr Short-term electrostimulation enhancing neural repair in vitro using large charge capacity nanostructured electrodes
title_full_unstemmed Short-term electrostimulation enhancing neural repair in vitro using large charge capacity nanostructured electrodes
title_sort Short-term electrostimulation enhancing neural repair in vitro using large charge capacity nanostructured electrodes
dc.creator.none.fl_str_mv Lichtenstein, Mathieu
Pérez Soler, Estela
Ballesteros, Luis Alberto
Suñol, Cristina
Casañ Pastor, Nieves
author Lichtenstein, Mathieu
author_facet Lichtenstein, Mathieu
Pérez Soler, Estela
Ballesteros, Luis Alberto
Suñol, Cristina
Casañ Pastor, Nieves
author_role author
author2 Pérez Soler, Estela
Ballesteros, Luis Alberto
Suñol, Cristina
Casañ Pastor, Nieves
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Educación y Cultura (España)
Ministerio de Economía y Competitividad (España)
Fundació La Marató de TV3
Consejo Superior de Investigaciones Científicas (España)
Instituto de Salud Carlos III
Generalitat de Catalunya
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Electrostimulation
Electrode materials
Charge capacity
In vitro neurite regeneration
Iridium oxide
Graphene
Conducting polymers
topic Electrostimulation
Electrode materials
Charge capacity
In vitro neurite regeneration
Iridium oxide
Graphene
Conducting polymers
description Electrostimulation of the neural system in functional or repair therapies requires new materials that protect the living system from electric field (EF) effects at the interface. Intercalation materials offer an alternative to radical formation during stimulation. Furthermore, nanostructuring of the electroactive material used as electrode offers an enlargement of the charge capacity which in turn involves changes in the EF effect. In this work, electric field stimulation of cortical neuron cultures has been applied in an in vitro model of lesion, namely, a physical scratch in the cell culture creates a cell-free area reminiscent of a lesion where new neurites grow. Regeneration of the “wound” zone upon EF stimulation is observed for various types of electrode materials, and compared to the spontaneous process and to platinum electrodes. Significantly, electric field effects are highly dependent on the electrode material used, even for the same charge delivered and similar impedance values. Electrode coatings with large charge storage capacity yield significantly better results than that of bare Pt electrodes. Neurite outgrowth at the scratched “wound” zone is lowest, below spontaneous regeneration, when using Pt electrodes. On the other hand, electroactive materials, such as bilayers of PEDOT and polypyrrole with lysine counterions or iridium oxide-pristine graphene hybrids, promote further regeneration. Beyond impedance considerations, the optimal material is the nanostructured one with the largest charge capacity, even at low charge deliveries. It is remarkable that IrOx-graphene hybrids reach regenerations above spontaneous case in very short stimulation times, for equal charge deliveries and potential protocols. The implications from the results suggest that EF application using these new coatings, may have an immediate use in safer electrostimulation procedures, and open routes for much needed neural repair.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/155964
url http://hdl.handle.net/10261/155964
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/MAT2015-65192-R
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SEV-2015-0496
http://dx.doi.org/10.1016/j.apmt.2016.12.002
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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