Dynamic and Regulated Association of Caveolin with Lipid Bodies: Modulation of Lipid Body Motility and Function by a Dominant Negative Mutant

Caveolins are a crucial component of caveolae but have also been localized to the Golgi complex, and, under some experimental conditions, to lipid bodies (LBs). The physiological relevance and dynamics of LB association remain unclear. We now show that endogenous caveolin-1 and caveolin-2 redistribu...

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Authors: Pol i Sorolla, Albert, Martin, Sally, Fernandez, Manuel A., Ferguson, Charles, Luetterforst, Robert, Enrich Bastús, Carles, Parton, Robert G., Carozzi, Amanda
Format: article
Status:Published version
Publication Date:2003
Country:España
Institution:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repository:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/25187
Online Access:https://hdl.handle.net/2445/25187
Access Level:Open access
Keyword:Proteïnes de membrana
Lípids
Membrane proteins
Lipids
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spelling Dynamic and Regulated Association of Caveolin with Lipid Bodies: Modulation of Lipid Body Motility and Function by a Dominant Negative MutantPol i Sorolla, AlbertMartin, SallyFernandez, Manuel A.Ferguson, CharlesLuetterforst, RobertEnrich Bastús, CarlesParton, Robert G.Carozzi, AmandaProteïnes de membranaLípidsMembrane proteinsLipidsCaveolins are a crucial component of caveolae but have also been localized to the Golgi complex, and, under some experimental conditions, to lipid bodies (LBs). The physiological relevance and dynamics of LB association remain unclear. We now show that endogenous caveolin-1 and caveolin-2 redistribute to LBs in lipid loaded A431 and FRT cells. Association with LBs is regulated and reversible; removal of fatty acids causes caveolin to rapidly leave the lipid body. We also show by subcellular fractionation, light and electron microscopy that during the first hours of liver regeneration, caveolins show a dramatic redistribution from the cell surface to the newly formed LBs. At later stages of the regeneration process (when LBs are still abundant), the levels of caveolins in LBs decrease dramatically. As a model system to study association of caveolins with LBs we have used brefeldin A (BFA). BFA causes rapid redistribution of endogenous caveolins to LBs and this association was reversed upon BFA washout. Finally, we have used a dominant negative LB-associated caveolin mutant (cavDGV) to study LB formation and to examine its effect on LB function. We now show that the cavDGV mutant inhibits microtubule-dependent LB motility and blocks the reversal of lipid accumulation in LBs.American Society for Cell Biology201220122003info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion12 p.application/pdfhttps://hdl.handle.net/2445/25187Articles publicats en revistes (Biomedicina)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: http://dx.doi.org/10.1091/mbc.E03-06-0368Molecular Biology of the Cell, 2004, vol. 15, núm. 1, p.99-110http://dx.doi.org/10.1091/mbc.E03-06-0368cc-by-nc-sa, (c) Pol et al., 2004http://creativecommons.org/licenses/cc-by-nc-sa/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/251872026-05-29T05:05:01Z
dc.title.none.fl_str_mv Dynamic and Regulated Association of Caveolin with Lipid Bodies: Modulation of Lipid Body Motility and Function by a Dominant Negative Mutant
title Dynamic and Regulated Association of Caveolin with Lipid Bodies: Modulation of Lipid Body Motility and Function by a Dominant Negative Mutant
spellingShingle Dynamic and Regulated Association of Caveolin with Lipid Bodies: Modulation of Lipid Body Motility and Function by a Dominant Negative Mutant
Pol i Sorolla, Albert
Proteïnes de membrana
Lípids
Membrane proteins
Lipids
title_short Dynamic and Regulated Association of Caveolin with Lipid Bodies: Modulation of Lipid Body Motility and Function by a Dominant Negative Mutant
title_full Dynamic and Regulated Association of Caveolin with Lipid Bodies: Modulation of Lipid Body Motility and Function by a Dominant Negative Mutant
title_fullStr Dynamic and Regulated Association of Caveolin with Lipid Bodies: Modulation of Lipid Body Motility and Function by a Dominant Negative Mutant
title_full_unstemmed Dynamic and Regulated Association of Caveolin with Lipid Bodies: Modulation of Lipid Body Motility and Function by a Dominant Negative Mutant
title_sort Dynamic and Regulated Association of Caveolin with Lipid Bodies: Modulation of Lipid Body Motility and Function by a Dominant Negative Mutant
dc.creator.none.fl_str_mv Pol i Sorolla, Albert
Martin, Sally
Fernandez, Manuel A.
Ferguson, Charles
Luetterforst, Robert
Enrich Bastús, Carles
Parton, Robert G.
Carozzi, Amanda
author Pol i Sorolla, Albert
author_facet Pol i Sorolla, Albert
Martin, Sally
Fernandez, Manuel A.
Ferguson, Charles
Luetterforst, Robert
Enrich Bastús, Carles
Parton, Robert G.
Carozzi, Amanda
author_role author
author2 Martin, Sally
Fernandez, Manuel A.
Ferguson, Charles
Luetterforst, Robert
Enrich Bastús, Carles
Parton, Robert G.
Carozzi, Amanda
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Proteïnes de membrana
Lípids
Membrane proteins
Lipids
topic Proteïnes de membrana
Lípids
Membrane proteins
Lipids
description Caveolins are a crucial component of caveolae but have also been localized to the Golgi complex, and, under some experimental conditions, to lipid bodies (LBs). The physiological relevance and dynamics of LB association remain unclear. We now show that endogenous caveolin-1 and caveolin-2 redistribute to LBs in lipid loaded A431 and FRT cells. Association with LBs is regulated and reversible; removal of fatty acids causes caveolin to rapidly leave the lipid body. We also show by subcellular fractionation, light and electron microscopy that during the first hours of liver regeneration, caveolins show a dramatic redistribution from the cell surface to the newly formed LBs. At later stages of the regeneration process (when LBs are still abundant), the levels of caveolins in LBs decrease dramatically. As a model system to study association of caveolins with LBs we have used brefeldin A (BFA). BFA causes rapid redistribution of endogenous caveolins to LBs and this association was reversed upon BFA washout. Finally, we have used a dominant negative LB-associated caveolin mutant (cavDGV) to study LB formation and to examine its effect on LB function. We now show that the cavDGV mutant inhibits microtubule-dependent LB motility and blocks the reversal of lipid accumulation in LBs.
publishDate 2003
dc.date.none.fl_str_mv 2003
2012
2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/25187
url https://hdl.handle.net/2445/25187
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1091/mbc.E03-06-0368
Molecular Biology of the Cell, 2004, vol. 15, núm. 1, p.99-110
http://dx.doi.org/10.1091/mbc.E03-06-0368
dc.rights.none.fl_str_mv cc-by-nc-sa, (c) Pol et al., 2004
http://creativecommons.org/licenses/cc-by-nc-sa/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-sa, (c) Pol et al., 2004
http://creativecommons.org/licenses/cc-by-nc-sa/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 12 p.
application/pdf
dc.publisher.none.fl_str_mv American Society for Cell Biology
publisher.none.fl_str_mv American Society for Cell Biology
dc.source.none.fl_str_mv Articles publicats en revistes (Biomedicina)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869414831498461184
score 15,812429

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