Bacterial diversity screening in endangered pelicans reveals high prevalence of methicillin-susceptible Staphylococcus aureus

In 2022, an avian influenza outbreak caused a massive mortality of Dalmatian pelicans (Pelecanus crispus, hereafter DP) in their world's largest breeding colony located at Mikri Prespa Lake (Greece), while great white pelicans (Pelecanus onocrotalus, hereafter GWP) were not affected. The aim of...

Descripción completa

Detalles Bibliográficos
Autores: Cardona Cabrera, Teresa, González Azcona, Carmen, Eguizábal, Paula, Alexandrou, Olga, Catsadorakis, Giorgos, Azmanis, Panagiotis, Lozano, Carmen, Höfle Hansen, Úrsula, Torres, Carmen
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Fundación Dialnet. Universidad de La Rioja
Repositorio:RUIdeRA. Repositorio Institucional de la UCLM
OAI Identifier:oai:ruidera.uclm.es:10578/47756
Acceso en línea:https://doi.org/10.1016/j.micpath.2025.108009
https://hdl.handle.net/10578/47756
Access Level:acceso abierto
Palabra clave:Dalmatian pelican
Great white pelican
Staphylococcus aureus
Bacterial diversity
Antimicrobial resistance
‘One health’
Greece
id ES_9e5c4102fcae5bcaaadaef7c414c235e
oai_identifier_str oai:ruidera.uclm.es:10578/47756
network_acronym_str ES
network_name_str España
repository_id_str
spelling Bacterial diversity screening in endangered pelicans reveals high prevalence of methicillin-susceptible Staphylococcus aureusCardona Cabrera, TeresaGonzález Azcona, CarmenEguizábal, PaulaAlexandrou, OlgaCatsadorakis, GiorgosAzmanis, PanagiotisLozano, CarmenHöfle Hansen, ÚrsulaTorres, CarmenDalmatian pelicanGreat white pelicanStaphylococcus aureusBacterial diversityAntimicrobial resistance‘One health’GreeceIn 2022, an avian influenza outbreak caused a massive mortality of Dalmatian pelicans (Pelecanus crispus, hereafter DP) in their world's largest breeding colony located at Mikri Prespa Lake (Greece), while great white pelicans (Pelecanus onocrotalus, hereafter GWP) were not affected. The aim of this study was to describe bacterial diversity and antimicrobial resistance carriage from choanal clefts of DPs and GWPs, to elucidate differences between the two species of pelicans and age groups. Fifty-two choanal swab samples were collected from 31 nestlings (N) (20 DP/11 GWP) late in the 2022 breeding season, and 21 adults (A) DPs (DP-A) in early 2023. Samples were seeded in general and chromogenic selective media. Isolates were identified by MALDI-TOF mass spectrometry and antimicrobial susceptibility was evaluated by disk diffusion method for staphylococci/mammaliicocci, enterococci and Enterobacterales. A total of 248 non-repetitive isolates (46A/202N) were recovered, representing 39 bacterial species from 18 genera. A very high prevalence of S. aureus (80.8 % of samples tested) was detected in both age groups (85.7 %A/77.4 %N), with nestlings also showing greater staphylococcal/mammaliicoccal diversity. Most isolates recovered were susceptible to all the antibiotics tested, and none S. aureus was methicillin-resistant. Nevertheless, one multi-drug resistant (MDR) CTX-M-15-producing E. coli-ST69 isolate was found in one DP-A. This study revealed a high rate of antimicrobial susceptibility and staphylococcal/mammaliicoccal diversity in choanal samples of pelicans, with the occasional detection of MDR isolates of concern, like the extended-spectrum beta-lactamase-producing E. coli strain.Elsevier202620262025info:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.1016/j.micpath.2025.108009https://hdl.handle.net/10578/47756reponame:RUIdeRA. Repositorio Institucional de la UCLMinstname:Fundación Dialnet. Universidad de La RiojaInglés20230896PID2022-1395910B-100info:eu-repo/semantics/openAccessoai:ruidera.uclm.es:10578/477562026-05-27T07:36:41Z
dc.title.none.fl_str_mv Bacterial diversity screening in endangered pelicans reveals high prevalence of methicillin-susceptible Staphylococcus aureus
title Bacterial diversity screening in endangered pelicans reveals high prevalence of methicillin-susceptible Staphylococcus aureus
spellingShingle Bacterial diversity screening in endangered pelicans reveals high prevalence of methicillin-susceptible Staphylococcus aureus
Cardona Cabrera, Teresa
Dalmatian pelican
Great white pelican
Staphylococcus aureus
Bacterial diversity
Antimicrobial resistance
‘One health’
Greece
title_short Bacterial diversity screening in endangered pelicans reveals high prevalence of methicillin-susceptible Staphylococcus aureus
title_full Bacterial diversity screening in endangered pelicans reveals high prevalence of methicillin-susceptible Staphylococcus aureus
title_fullStr Bacterial diversity screening in endangered pelicans reveals high prevalence of methicillin-susceptible Staphylococcus aureus
title_full_unstemmed Bacterial diversity screening in endangered pelicans reveals high prevalence of methicillin-susceptible Staphylococcus aureus
title_sort Bacterial diversity screening in endangered pelicans reveals high prevalence of methicillin-susceptible Staphylococcus aureus
dc.creator.none.fl_str_mv Cardona Cabrera, Teresa
González Azcona, Carmen
Eguizábal, Paula
Alexandrou, Olga
Catsadorakis, Giorgos
Azmanis, Panagiotis
Lozano, Carmen
Höfle Hansen, Úrsula
Torres, Carmen
author Cardona Cabrera, Teresa
author_facet Cardona Cabrera, Teresa
González Azcona, Carmen
Eguizábal, Paula
Alexandrou, Olga
Catsadorakis, Giorgos
Azmanis, Panagiotis
Lozano, Carmen
Höfle Hansen, Úrsula
Torres, Carmen
author_role author
author2 González Azcona, Carmen
Eguizábal, Paula
Alexandrou, Olga
Catsadorakis, Giorgos
Azmanis, Panagiotis
Lozano, Carmen
Höfle Hansen, Úrsula
Torres, Carmen
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Dalmatian pelican
Great white pelican
Staphylococcus aureus
Bacterial diversity
Antimicrobial resistance
‘One health’
Greece
topic Dalmatian pelican
Great white pelican
Staphylococcus aureus
Bacterial diversity
Antimicrobial resistance
‘One health’
Greece
description In 2022, an avian influenza outbreak caused a massive mortality of Dalmatian pelicans (Pelecanus crispus, hereafter DP) in their world's largest breeding colony located at Mikri Prespa Lake (Greece), while great white pelicans (Pelecanus onocrotalus, hereafter GWP) were not affected. The aim of this study was to describe bacterial diversity and antimicrobial resistance carriage from choanal clefts of DPs and GWPs, to elucidate differences between the two species of pelicans and age groups. Fifty-two choanal swab samples were collected from 31 nestlings (N) (20 DP/11 GWP) late in the 2022 breeding season, and 21 adults (A) DPs (DP-A) in early 2023. Samples were seeded in general and chromogenic selective media. Isolates were identified by MALDI-TOF mass spectrometry and antimicrobial susceptibility was evaluated by disk diffusion method for staphylococci/mammaliicocci, enterococci and Enterobacterales. A total of 248 non-repetitive isolates (46A/202N) were recovered, representing 39 bacterial species from 18 genera. A very high prevalence of S. aureus (80.8 % of samples tested) was detected in both age groups (85.7 %A/77.4 %N), with nestlings also showing greater staphylococcal/mammaliicoccal diversity. Most isolates recovered were susceptible to all the antibiotics tested, and none S. aureus was methicillin-resistant. Nevertheless, one multi-drug resistant (MDR) CTX-M-15-producing E. coli-ST69 isolate was found in one DP-A. This study revealed a high rate of antimicrobial susceptibility and staphylococcal/mammaliicoccal diversity in choanal samples of pelicans, with the occasional detection of MDR isolates of concern, like the extended-spectrum beta-lactamase-producing E. coli strain.
publishDate 2025
dc.date.none.fl_str_mv 2025
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://doi.org/10.1016/j.micpath.2025.108009
https://hdl.handle.net/10578/47756
url https://doi.org/10.1016/j.micpath.2025.108009
https://hdl.handle.net/10578/47756
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv 20230896
PID2022-1395910B-100
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:RUIdeRA. Repositorio Institucional de la UCLM
instname:Fundación Dialnet. Universidad de La Rioja
instname_str Fundación Dialnet. Universidad de La Rioja
reponame_str RUIdeRA. Repositorio Institucional de la UCLM
collection RUIdeRA. Repositorio Institucional de la UCLM
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869414820255629312
score 15,811543

Similares en LA Referencia