1-Deoxy-D-xylulose 5-phosphate reductoisomerase and plastid isoprenoid biosynthesis during tomato fruit ripening

The recently discovered 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway for the biosynthesis of plastid isoprenoids (including carotenoids) is not fully elucidated yet despite its central importance for plant life. It is known, however, that the first reaction completely specific to the pathway is...

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Autores: Rodríguez Concepción, Manuel, Ahumada, Iván, Diez Juez, Ester, Sauret-Güeto, Susanna, Lois Rojas, Luisa María, Gallego, Francisco, Carretero Paulet, Lorenzo, Campos Martínez, Narciso, Boronat i Margosa, Albert
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2001
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/226010
Acceso en línea:https://hdl.handle.net/2445/226010
Access Level:acceso abierto
Palabra clave:Biologia molecular vegetal
Tomàquets
Biotecnologia vegetal
Plant molecular biology
Tomatoes
Plant biotechnology
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spelling 1-Deoxy-D-xylulose 5-phosphate reductoisomerase and plastid isoprenoid biosynthesis during tomato fruit ripeningRodríguez Concepción, ManuelAhumada, IvánDiez Juez, EsterSauret-Güeto, SusannaLois Rojas, Luisa MaríaGallego, FranciscoCarretero Paulet, LorenzoCampos Martínez, NarcisoBoronat i Margosa, AlbertBiologia molecular vegetalTomàquetsBiotecnologia vegetalPlant molecular biologyTomatoesPlant biotechnologyThe recently discovered 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway for the biosynthesis of plastid isoprenoids (including carotenoids) is not fully elucidated yet despite its central importance for plant life. It is known, however, that the first reaction completely specific to the pathway is the conversion of 1-deoxy-D-xylulose 5-phosphate (DXP) into MEP by the enzyme DXP reductoisomerase (DXR). We have identified a tomato cDNA encoding a protein with homology to DXR and in vivo activity, and show that the levels of the corresponding DXR mRNA and encoded protein in fruit tissues are similar before and during the massive accumulation of carotenoids characteristic of fruit ripening. The results are consistent with a non-limiting role of DXR, and support previous work proposing DXP synthase (DXS) as the first regulatory enzyme for plastid isoprenoid biosynthesis in tomato fruit. Inhibition of DXR activity by fosmidomycin showed that plastid isoprenoid biosynthesis is required for tomato fruit carotenogenesis but not for other ripening processes. In addition, dormancy was reduced in seeds from fosmidomycin-treated fruit but not in seeds from the tomato yellow ripe mutant (defective in phytoene synthase-1, PSY1), suggesting that the isoform PSY2 might channel the production of carotenoids for abscisic acid biosynthesis. Furthermore, the complete arrest of tomato seedling development using fosmidomycin confirms a key role of the MEP pathway in plant development.John Wiley & Sons2001info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/226010Articles publicats en revistes (Bioquímica i Biomedicina Molecular)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésVersió postprint del document publicat a: https://doi.org/10.1046/j.1365-313x.2001.01089.xPlant Journal, 2001, vol. 27, num.3, p. 213-222https://doi.org/10.1046/j.1365-313x.2001.01089.x(c) John Wiley & Sons, 2001info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/2260102026-05-27T06:46:51Z
dc.title.none.fl_str_mv 1-Deoxy-D-xylulose 5-phosphate reductoisomerase and plastid isoprenoid biosynthesis during tomato fruit ripening
title 1-Deoxy-D-xylulose 5-phosphate reductoisomerase and plastid isoprenoid biosynthesis during tomato fruit ripening
spellingShingle 1-Deoxy-D-xylulose 5-phosphate reductoisomerase and plastid isoprenoid biosynthesis during tomato fruit ripening
Rodríguez Concepción, Manuel
Biologia molecular vegetal
Tomàquets
Biotecnologia vegetal
Plant molecular biology
Tomatoes
Plant biotechnology
title_short 1-Deoxy-D-xylulose 5-phosphate reductoisomerase and plastid isoprenoid biosynthesis during tomato fruit ripening
title_full 1-Deoxy-D-xylulose 5-phosphate reductoisomerase and plastid isoprenoid biosynthesis during tomato fruit ripening
title_fullStr 1-Deoxy-D-xylulose 5-phosphate reductoisomerase and plastid isoprenoid biosynthesis during tomato fruit ripening
title_full_unstemmed 1-Deoxy-D-xylulose 5-phosphate reductoisomerase and plastid isoprenoid biosynthesis during tomato fruit ripening
title_sort 1-Deoxy-D-xylulose 5-phosphate reductoisomerase and plastid isoprenoid biosynthesis during tomato fruit ripening
dc.creator.none.fl_str_mv Rodríguez Concepción, Manuel
Ahumada, Iván
Diez Juez, Ester
Sauret-Güeto, Susanna
Lois Rojas, Luisa María
Gallego, Francisco
Carretero Paulet, Lorenzo
Campos Martínez, Narciso
Boronat i Margosa, Albert
author Rodríguez Concepción, Manuel
author_facet Rodríguez Concepción, Manuel
Ahumada, Iván
Diez Juez, Ester
Sauret-Güeto, Susanna
Lois Rojas, Luisa María
Gallego, Francisco
Carretero Paulet, Lorenzo
Campos Martínez, Narciso
Boronat i Margosa, Albert
author_role author
author2 Ahumada, Iván
Diez Juez, Ester
Sauret-Güeto, Susanna
Lois Rojas, Luisa María
Gallego, Francisco
Carretero Paulet, Lorenzo
Campos Martínez, Narciso
Boronat i Margosa, Albert
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Biologia molecular vegetal
Tomàquets
Biotecnologia vegetal
Plant molecular biology
Tomatoes
Plant biotechnology
topic Biologia molecular vegetal
Tomàquets
Biotecnologia vegetal
Plant molecular biology
Tomatoes
Plant biotechnology
description The recently discovered 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway for the biosynthesis of plastid isoprenoids (including carotenoids) is not fully elucidated yet despite its central importance for plant life. It is known, however, that the first reaction completely specific to the pathway is the conversion of 1-deoxy-D-xylulose 5-phosphate (DXP) into MEP by the enzyme DXP reductoisomerase (DXR). We have identified a tomato cDNA encoding a protein with homology to DXR and in vivo activity, and show that the levels of the corresponding DXR mRNA and encoded protein in fruit tissues are similar before and during the massive accumulation of carotenoids characteristic of fruit ripening. The results are consistent with a non-limiting role of DXR, and support previous work proposing DXP synthase (DXS) as the first regulatory enzyme for plastid isoprenoid biosynthesis in tomato fruit. Inhibition of DXR activity by fosmidomycin showed that plastid isoprenoid biosynthesis is required for tomato fruit carotenogenesis but not for other ripening processes. In addition, dormancy was reduced in seeds from fosmidomycin-treated fruit but not in seeds from the tomato yellow ripe mutant (defective in phytoene synthase-1, PSY1), suggesting that the isoform PSY2 might channel the production of carotenoids for abscisic acid biosynthesis. Furthermore, the complete arrest of tomato seedling development using fosmidomycin confirms a key role of the MEP pathway in plant development.
publishDate 2001
dc.date.none.fl_str_mv 2001
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/226010
url https://hdl.handle.net/2445/226010
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1046/j.1365-313x.2001.01089.x
Plant Journal, 2001, vol. 27, num.3, p. 213-222
https://doi.org/10.1046/j.1365-313x.2001.01089.x
dc.rights.none.fl_str_mv (c) John Wiley & Sons, 2001
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) John Wiley & Sons, 2001
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Biomedicina Molecular)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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