In vitro evaluation of gentamicin activity against Spanish field isolates of Brachyspira hyodysenteriae
[EN]Background: The treatment of swine dysentery (SD) has become constrained in recent years due to the limited availability of effective drugs combined with a rise in antimicrobial resistance. Gentamicin, an aminoglycoside antibiotic, is authorised for the control of this disease in several Europea...
| Autores: | , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Universidad de León |
| Repositorio: | BULERIA. Repositorio Institucional de la Universidad de León |
| OAI Identifier: | oai:buleria.unileon.es:10612/17882 |
| Acceso en línea: | https://porcinehealthmanagement.biomedcentral.com/articles/10.1186/s40813-022-00291-w#citeas https://hdl.handle.net/10612/17882 |
| Access Level: | acceso abierto |
| Palabra clave: | Sanidad animal Spirochaetes Antibiotic Aminoglycoside Swine dysentery Antimicrobial susceptibility testing Resistance 3109 Ciencias Veterinarias 3104.08 Porcinos |
| id |
ES_9e40aeecf945e0fbe957ea2f448b66da |
|---|---|
| oai_identifier_str |
oai:buleria.unileon.es:10612/17882 |
| network_acronym_str |
ES |
| network_name_str |
España |
| repository_id_str |
|
| spelling |
In vitro evaluation of gentamicin activity against Spanish field isolates of Brachyspira hyodysenteriaeVega, ClaraPérez Pérez, LucíaArgüello Rodríguez, HéctorGómez García, ManuelPuente Fernández, HéctorFernández-Usón, IgnacioRubio Nistal, Pedro MiguelCarvajal Urueña, Ana MaríaSanidad animalSpirochaetesAntibioticAminoglycosideSwine dysenteryAntimicrobial susceptibility testingResistance3109 Ciencias Veterinarias3104.08 Porcinos[EN]Background: The treatment of swine dysentery (SD) has become constrained in recent years due to the limited availability of effective drugs combined with a rise in antimicrobial resistance. Gentamicin, an aminoglycoside antibiotic, is authorised for the control of this disease in several European countries but has not been extensively used so far. In this study, the in vitro susceptibility of 56 Brachyspira hyodysenteriae field isolates was evaluated against gentamicin using a broth microdilution test. The molecular basis of decreased susceptibility to gentamicin was also investigated by sequencing the 16S rRNA gene and phylogenetic relatedness by multiple-locus variable number tandem-repeat analysis (MLVA). Results: Most B. hyodysenteriae isolates presented low minimum inhibitory concentration (MIC) values to gentamicin, with a mode of 2 µg/mL, a median or MIC50 of 4 µg/mL and percentile 90 or MIC90 of 16 µg/mL. The distribution of these values over the period studied (2011–2019) did not show a tendency towards the development of resistance to gentamicin. Differences in susceptibility among isolates could be explained by two point-mutations in the 16S rRNA gene, C990T and A1185G, which were only present in isolates with high MICs. These isolates were typed in three different MLVA clusters. Analyses of co-resistance between gentamicin and antimicrobials commonly used for the treatment of SD revealed that resistance to tiamulin and valnemulin was associated with low MICs for gentamicin. Conclusions: The results provide an accurate characterisation of antimicrobial sensitivity to gentamicin and possible mechanisms of resistance in Spanish B. hyodysenteriae isolates. These findings allow us to propose gentamicin as an alternative in the antibiotic management of SD, particularly in outbreaks caused by pleuromutilin resistant isolates.SIThe authors wish to thank Benjamín Rabanal for his contribution in some parts of the research and Diana Molina for her excellent technical assistance. Clara Vega (workplace ULE-02-B) and Manuel Gómez (LE131-18) are supported by grants from Junta de Castilla y León co-financed by the European Social Fund and Youth Employment Initiative. Héctor Argüello is supported by a grant from the Spanish Ministry of Education under the programme “Beatriz Galindo” (BEAGAL-18-106). Lucía Pérez-Pérez (PRE2020-093762) and Héctor Puente (FPU17/00466) are also supported by the Spanish Ministry of Education. We fnally want to thank the reviewer for the advice and ideas provided which clearly improved the quality of the manuscript.This work was supported by Fatro Ibérica S.L.BioMed CentralSanidad AnimalFacultad de Veterinaria2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://porcinehealthmanagement.biomedcentral.com/articles/10.1186/s40813-022-00291-w#citeashttps://hdl.handle.net/10612/17882reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad de LeónIngléshttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/178822026-06-24T12:43:27Z |
| dc.title.none.fl_str_mv |
In vitro evaluation of gentamicin activity against Spanish field isolates of Brachyspira hyodysenteriae |
| title |
In vitro evaluation of gentamicin activity against Spanish field isolates of Brachyspira hyodysenteriae |
| spellingShingle |
In vitro evaluation of gentamicin activity against Spanish field isolates of Brachyspira hyodysenteriae Vega, Clara Sanidad animal Spirochaetes Antibiotic Aminoglycoside Swine dysentery Antimicrobial susceptibility testing Resistance 3109 Ciencias Veterinarias 3104.08 Porcinos |
| title_short |
In vitro evaluation of gentamicin activity against Spanish field isolates of Brachyspira hyodysenteriae |
| title_full |
In vitro evaluation of gentamicin activity against Spanish field isolates of Brachyspira hyodysenteriae |
| title_fullStr |
In vitro evaluation of gentamicin activity against Spanish field isolates of Brachyspira hyodysenteriae |
| title_full_unstemmed |
In vitro evaluation of gentamicin activity against Spanish field isolates of Brachyspira hyodysenteriae |
| title_sort |
In vitro evaluation of gentamicin activity against Spanish field isolates of Brachyspira hyodysenteriae |
| dc.creator.none.fl_str_mv |
Vega, Clara Pérez Pérez, Lucía Argüello Rodríguez, Héctor Gómez García, Manuel Puente Fernández, Héctor Fernández-Usón, Ignacio Rubio Nistal, Pedro Miguel Carvajal Urueña, Ana María |
| author |
Vega, Clara |
| author_facet |
Vega, Clara Pérez Pérez, Lucía Argüello Rodríguez, Héctor Gómez García, Manuel Puente Fernández, Héctor Fernández-Usón, Ignacio Rubio Nistal, Pedro Miguel Carvajal Urueña, Ana María |
| author_role |
author |
| author2 |
Pérez Pérez, Lucía Argüello Rodríguez, Héctor Gómez García, Manuel Puente Fernández, Héctor Fernández-Usón, Ignacio Rubio Nistal, Pedro Miguel Carvajal Urueña, Ana María |
| author2_role |
author author author author author author author |
| dc.contributor.none.fl_str_mv |
Sanidad Animal Facultad de Veterinaria |
| dc.subject.none.fl_str_mv |
Sanidad animal Spirochaetes Antibiotic Aminoglycoside Swine dysentery Antimicrobial susceptibility testing Resistance 3109 Ciencias Veterinarias 3104.08 Porcinos |
| topic |
Sanidad animal Spirochaetes Antibiotic Aminoglycoside Swine dysentery Antimicrobial susceptibility testing Resistance 3109 Ciencias Veterinarias 3104.08 Porcinos |
| description |
[EN]Background: The treatment of swine dysentery (SD) has become constrained in recent years due to the limited availability of effective drugs combined with a rise in antimicrobial resistance. Gentamicin, an aminoglycoside antibiotic, is authorised for the control of this disease in several European countries but has not been extensively used so far. In this study, the in vitro susceptibility of 56 Brachyspira hyodysenteriae field isolates was evaluated against gentamicin using a broth microdilution test. The molecular basis of decreased susceptibility to gentamicin was also investigated by sequencing the 16S rRNA gene and phylogenetic relatedness by multiple-locus variable number tandem-repeat analysis (MLVA). Results: Most B. hyodysenteriae isolates presented low minimum inhibitory concentration (MIC) values to gentamicin, with a mode of 2 µg/mL, a median or MIC50 of 4 µg/mL and percentile 90 or MIC90 of 16 µg/mL. The distribution of these values over the period studied (2011–2019) did not show a tendency towards the development of resistance to gentamicin. Differences in susceptibility among isolates could be explained by two point-mutations in the 16S rRNA gene, C990T and A1185G, which were only present in isolates with high MICs. These isolates were typed in three different MLVA clusters. Analyses of co-resistance between gentamicin and antimicrobials commonly used for the treatment of SD revealed that resistance to tiamulin and valnemulin was associated with low MICs for gentamicin. Conclusions: The results provide an accurate characterisation of antimicrobial sensitivity to gentamicin and possible mechanisms of resistance in Spanish B. hyodysenteriae isolates. These findings allow us to propose gentamicin as an alternative in the antibiotic management of SD, particularly in outbreaks caused by pleuromutilin resistant isolates. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://porcinehealthmanagement.biomedcentral.com/articles/10.1186/s40813-022-00291-w#citeas https://hdl.handle.net/10612/17882 |
| url |
https://porcinehealthmanagement.biomedcentral.com/articles/10.1186/s40813-022-00291-w#citeas https://hdl.handle.net/10612/17882 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.rights.none.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
BioMed Central |
| publisher.none.fl_str_mv |
BioMed Central |
| dc.source.none.fl_str_mv |
reponame:BULERIA. Repositorio Institucional de la Universidad de León instname:Universidad de León |
| instname_str |
Universidad de León |
| reponame_str |
BULERIA. Repositorio Institucional de la Universidad de León |
| collection |
BULERIA. Repositorio Institucional de la Universidad de León |
| repository.name.fl_str_mv |
|
| repository.mail.fl_str_mv |
|
| _version_ |
1869414811208515584 |
| score |
15.300719 |