Novel tissue engineering strategies based on the combination of the polymeric devices and adult stem cells for cardiac repair

Cardiovascular diseases (CVD) are the leading cause of death worldwide. Among them, myocardial infarction (MI) is the main CVDs, causing 7.4 million deaths each year. The current therapies for MI are palliative rather than regenerative. Since that, the tissue engineering (TE) strategy has grown inve...

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Autor: Díaz-Herráez, P. (Paula)|||/items/b2b37f65-3fe4-464a-942c-bd20de1eb2cc
Tipo de recurso: tesis doctoral
Fecha de publicación:2015
País:España
Institución:Universidad de Navarra
Repositorio:Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:inglés
OAI Identifier:oai:dadun.unav.edu:10171/58712
Acceso en línea:https://hdl.handle.net/10171/58712
Access Level:acceso abierto
Palabra clave:Evaluación de fármacos
Materias Investigacion::Farmacia::Farmacia y farmacología
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spelling Novel tissue engineering strategies based on the combination of the polymeric devices and adult stem cells for cardiac repairDíaz-Herráez, P. (Paula)|||/items/b2b37f65-3fe4-464a-942c-bd20de1eb2ccEvaluación de fármacosMaterias Investigacion::Farmacia::Farmacia y farmacologíaCardiovascular diseases (CVD) are the leading cause of death worldwide. Among them, myocardial infarction (MI) is the main CVDs, causing 7.4 million deaths each year. The current therapies for MI are palliative rather than regenerative. Since that, the tissue engineering (TE) strategy has grown investigators attention. With the aim of improving heart repair after MI, we have developed two TE strategies. The first strategy was the use of poly(lactic co-glycolic acid) (PLGA) microparticles (MPs) containing neuregulin (NRG), as support for attaching adipose-derived stem cells (ADSC): ADSC-NRG-MP. The second strategy was the combination of two different polymeric devices, MPs and hydrogels. The MPs loaded with NRG were embedded together with the ADSCs in hydrogels composed of different ratios of dextran (Dex) and hyaluronic acid (HA). Our hypothesis was that the developed systems would increase cell survival and would activate different pathways to favor heart regeneration. The ADSC-NRG-MP induced a more pronounced regeneration of the infarcted heart in a rat MI model (reduction in infarct size, higher left ventricle thickness and vasculogenesis). Also the employment of MPs as support for the ADSC, favored a long-term survival of the cells once in the tissue, being detectable three months after their administration. Moreover, among the hydrogels developed, the 50:50 Dex:HA hydrogel embedding 1 mg of NRG-MP and 500,000 ADSC showed to present the best characteristics (adequate stiffness for heart administration, prolonged gelation time to allow its injectability and degradation rate to allow cell survival). To conclude, the two strategies developed have shown to be promising candidates for heart repair after a MI.Blanco-Prieto, M.J. (María José)Garbayo, E. (Elisa)Dadun. Depósito Académico Digital Universidad de Navarra20202020-01-3020202020-01-3020202020-01-3020152015-10-09doctoral thesishttp://purl.org/coar/resource_type/c_db06info:eu-repo/semantics/doctoralThesisapplication/pdfhttps://hdl.handle.net/10171/58712reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/587122026-06-21T12:47:57Z
dc.title.none.fl_str_mv Novel tissue engineering strategies based on the combination of the polymeric devices and adult stem cells for cardiac repair
title Novel tissue engineering strategies based on the combination of the polymeric devices and adult stem cells for cardiac repair
spellingShingle Novel tissue engineering strategies based on the combination of the polymeric devices and adult stem cells for cardiac repair
Díaz-Herráez, P. (Paula)|||/items/b2b37f65-3fe4-464a-942c-bd20de1eb2cc
Evaluación de fármacos
Materias Investigacion::Farmacia::Farmacia y farmacología
title_short Novel tissue engineering strategies based on the combination of the polymeric devices and adult stem cells for cardiac repair
title_full Novel tissue engineering strategies based on the combination of the polymeric devices and adult stem cells for cardiac repair
title_fullStr Novel tissue engineering strategies based on the combination of the polymeric devices and adult stem cells for cardiac repair
title_full_unstemmed Novel tissue engineering strategies based on the combination of the polymeric devices and adult stem cells for cardiac repair
title_sort Novel tissue engineering strategies based on the combination of the polymeric devices and adult stem cells for cardiac repair
dc.creator.none.fl_str_mv Díaz-Herráez, P. (Paula)|||/items/b2b37f65-3fe4-464a-942c-bd20de1eb2cc
author Díaz-Herráez, P. (Paula)|||/items/b2b37f65-3fe4-464a-942c-bd20de1eb2cc
author_facet Díaz-Herráez, P. (Paula)|||/items/b2b37f65-3fe4-464a-942c-bd20de1eb2cc
author_role author
dc.contributor.none.fl_str_mv Blanco-Prieto, M.J. (María José)
Garbayo, E. (Elisa)
Dadun. Depósito Académico Digital Universidad de Navarra
dc.subject.none.fl_str_mv Evaluación de fármacos
Materias Investigacion::Farmacia::Farmacia y farmacología
topic Evaluación de fármacos
Materias Investigacion::Farmacia::Farmacia y farmacología
description Cardiovascular diseases (CVD) are the leading cause of death worldwide. Among them, myocardial infarction (MI) is the main CVDs, causing 7.4 million deaths each year. The current therapies for MI are palliative rather than regenerative. Since that, the tissue engineering (TE) strategy has grown investigators attention. With the aim of improving heart repair after MI, we have developed two TE strategies. The first strategy was the use of poly(lactic co-glycolic acid) (PLGA) microparticles (MPs) containing neuregulin (NRG), as support for attaching adipose-derived stem cells (ADSC): ADSC-NRG-MP. The second strategy was the combination of two different polymeric devices, MPs and hydrogels. The MPs loaded with NRG were embedded together with the ADSCs in hydrogels composed of different ratios of dextran (Dex) and hyaluronic acid (HA). Our hypothesis was that the developed systems would increase cell survival and would activate different pathways to favor heart regeneration. The ADSC-NRG-MP induced a more pronounced regeneration of the infarcted heart in a rat MI model (reduction in infarct size, higher left ventricle thickness and vasculogenesis). Also the employment of MPs as support for the ADSC, favored a long-term survival of the cells once in the tissue, being detectable three months after their administration. Moreover, among the hydrogels developed, the 50:50 Dex:HA hydrogel embedding 1 mg of NRG-MP and 500,000 ADSC showed to present the best characteristics (adequate stiffness for heart administration, prolonged gelation time to allow its injectability and degradation rate to allow cell survival). To conclude, the two strategies developed have shown to be promising candidates for heart repair after a MI.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-10-09
2020
2020-01-30
2020
2020-01-30
2020
2020-01-30
dc.type.none.fl_str_mv doctoral thesis
http://purl.org/coar/resource_type/c_db06
dc.type.openaire.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
dc.identifier.none.fl_str_mv https://hdl.handle.net/10171/58712
url https://hdl.handle.net/10171/58712
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra
instname:Universidad de Navarra
instname_str Universidad de Navarra
reponame_str Dadun. Depósito Académico Digital de la Universidad de Navarra
collection Dadun. Depósito Académico Digital de la Universidad de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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