The Transcriptome Analysis of Strongyloides stercoralis L3i Larvae Reveals Targets for Intervention in a Neglected Disease

Background: Strongyloidiasis is one of the most neglected diseases distributed worldwide with endemic areas in developed countries, where chronic infections are life threatening. Despite its impact, very little is known about the molecular biology of the parasite involved and its interplay with its...

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Autores: Marcilla, Antonio, Garg, Gagan, Bernal, Dolores, Ranganathan, Shoba, Forment, Javier, Ortiz, Javier, Muñoz-Antolí, Carla, Domínguez, M. Victoria, Pedrola, Laia, Martínez-Blanch, Juan F., Sotillo, Javier, Trelis, Maria, Toledo, Rafael, Esteban, J. Guillermo
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2012
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/48616
Acesso em linha:http://hdl.handle.net/10261/48616
Access Level:Acceso aberto
Palavra-chave:Dictyocaulus-viviparus
Haemonchus-contortus
Functional genomics
Helminth-parasites
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spelling The Transcriptome Analysis of Strongyloides stercoralis L3i Larvae Reveals Targets for Intervention in a Neglected DiseaseMarcilla, AntonioGarg, GaganBernal, DoloresRanganathan, ShobaForment, JavierOrtiz, JavierMuñoz-Antolí, CarlaDomínguez, M. VictoriaPedrola, LaiaMartínez-Blanch, Juan F.Sotillo, JavierTrelis, MariaToledo, RafaelEsteban, J. GuillermoDictyocaulus-viviparusHaemonchus-contortusFunctional genomicsHelminth-parasitesBackground: Strongyloidiasis is one of the most neglected diseases distributed worldwide with endemic areas in developed countries, where chronic infections are life threatening. Despite its impact, very little is known about the molecular biology of the parasite involved and its interplay with its hosts. Next generation sequencing technologies now provide unique opportunities to rapidly address these questions. Principal Findings: Here we present the first transcriptome of the third larval stage of S. stercoralis using 454 sequencing coupled with semi-automated bioinformatic analyses. 253,266 raw sequence reads were assembled into 11,250 contiguous sequences, most of which were novel. 8037 putative proteins were characterized based on homology, gene ontology and/ or biochemical pathways. Comparison of the transcriptome of S. strongyloides with those of other nematodes, including S. ratti, revealed similarities in transcription of molecules inferred to have key roles in parasite-host interactions. Enzymatic proteins, like kinases and proteases, were abundant. 1213 putative excretory/secretory proteins were compiled using a new pipeline which included non-classical secretory proteins. Potential drug targets were also identified. Conclusions: Overall, the present dataset should provide a solid foundation for future fundamental genomic, proteomic and metabolomic explorations of S. stercoralis, as well as a basis for applied outcomes, such as the development of novel methods of intervention against this neglected parasite.This work was supported by project PS09/02355 from the Fondo de Investigacio´n Sanitaria (FIS), Spanish Ministry of Science and Innovation (Madrid, Spain) and FEDER and project PROMETEO/2009/081 from Conselleria d’Educacio´ , Generalitat Valenciana (Valencia, Spain). GG would like to acknowledge Macquarie University for an Australian Post-graduate Award scholarship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Peer reviewedPublic Library of Science201220122012info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/48616reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglésinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/486162026-05-22T06:33:51Z
dc.title.none.fl_str_mv The Transcriptome Analysis of Strongyloides stercoralis L3i Larvae Reveals Targets for Intervention in a Neglected Disease
title The Transcriptome Analysis of Strongyloides stercoralis L3i Larvae Reveals Targets for Intervention in a Neglected Disease
spellingShingle The Transcriptome Analysis of Strongyloides stercoralis L3i Larvae Reveals Targets for Intervention in a Neglected Disease
Marcilla, Antonio
Dictyocaulus-viviparus
Haemonchus-contortus
Functional genomics
Helminth-parasites
title_short The Transcriptome Analysis of Strongyloides stercoralis L3i Larvae Reveals Targets for Intervention in a Neglected Disease
title_full The Transcriptome Analysis of Strongyloides stercoralis L3i Larvae Reveals Targets for Intervention in a Neglected Disease
title_fullStr The Transcriptome Analysis of Strongyloides stercoralis L3i Larvae Reveals Targets for Intervention in a Neglected Disease
title_full_unstemmed The Transcriptome Analysis of Strongyloides stercoralis L3i Larvae Reveals Targets for Intervention in a Neglected Disease
title_sort The Transcriptome Analysis of Strongyloides stercoralis L3i Larvae Reveals Targets for Intervention in a Neglected Disease
dc.creator.none.fl_str_mv Marcilla, Antonio
Garg, Gagan
Bernal, Dolores
Ranganathan, Shoba
Forment, Javier
Ortiz, Javier
Muñoz-Antolí, Carla
Domínguez, M. Victoria
Pedrola, Laia
Martínez-Blanch, Juan F.
Sotillo, Javier
Trelis, Maria
Toledo, Rafael
Esteban, J. Guillermo
author Marcilla, Antonio
author_facet Marcilla, Antonio
Garg, Gagan
Bernal, Dolores
Ranganathan, Shoba
Forment, Javier
Ortiz, Javier
Muñoz-Antolí, Carla
Domínguez, M. Victoria
Pedrola, Laia
Martínez-Blanch, Juan F.
Sotillo, Javier
Trelis, Maria
Toledo, Rafael
Esteban, J. Guillermo
author_role author
author2 Garg, Gagan
Bernal, Dolores
Ranganathan, Shoba
Forment, Javier
Ortiz, Javier
Muñoz-Antolí, Carla
Domínguez, M. Victoria
Pedrola, Laia
Martínez-Blanch, Juan F.
Sotillo, Javier
Trelis, Maria
Toledo, Rafael
Esteban, J. Guillermo
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Dictyocaulus-viviparus
Haemonchus-contortus
Functional genomics
Helminth-parasites
topic Dictyocaulus-viviparus
Haemonchus-contortus
Functional genomics
Helminth-parasites
description Background: Strongyloidiasis is one of the most neglected diseases distributed worldwide with endemic areas in developed countries, where chronic infections are life threatening. Despite its impact, very little is known about the molecular biology of the parasite involved and its interplay with its hosts. Next generation sequencing technologies now provide unique opportunities to rapidly address these questions. Principal Findings: Here we present the first transcriptome of the third larval stage of S. stercoralis using 454 sequencing coupled with semi-automated bioinformatic analyses. 253,266 raw sequence reads were assembled into 11,250 contiguous sequences, most of which were novel. 8037 putative proteins were characterized based on homology, gene ontology and/ or biochemical pathways. Comparison of the transcriptome of S. strongyloides with those of other nematodes, including S. ratti, revealed similarities in transcription of molecules inferred to have key roles in parasite-host interactions. Enzymatic proteins, like kinases and proteases, were abundant. 1213 putative excretory/secretory proteins were compiled using a new pipeline which included non-classical secretory proteins. Potential drug targets were also identified. Conclusions: Overall, the present dataset should provide a solid foundation for future fundamental genomic, proteomic and metabolomic explorations of S. stercoralis, as well as a basis for applied outcomes, such as the development of novel methods of intervention against this neglected parasite.
publishDate 2012
dc.date.none.fl_str_mv 2012
2012
2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/48616
url http://hdl.handle.net/10261/48616
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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